MiR-155 enhances phagocytic activity of β-thalassemia/HbE monocytes via targeting of BACH1

Abnormal red blood cell (RBC) clearance in beta-thalassemia is triggered by activated monocytes. Recent reports indicate that miRNA (miR-) plays a role in monocyte activation. To study phagocytic function, we co-cultured monocytes of normal, non-splenectomized and splenectomized beta-thalassemia/HbE individuals with RBCs obtained from normal, non-splenectomized and splenectomized beta-thalassemia/HbE individuals. The phagocytic activity of beta-thalassemia/HbE monocytes co-cultured with beta-thalassemia/HbE RBCs was significantly higher than that of normal monocytes co-cultured with normal RBCs. Upregulation of monocyte miR-155 was observed in beta-thalassemia/HbE patients. Increased miR-155 was associated with reductions in BTB and CNC Homology1 (BACH1) target gene expression and increased phagocytic activity of beta-thalassemia/HbE monocytes. Taken together, these findings suggested that increased miR-155 expression in activated monocytes leads to enhanced phagocytic activity via BACH-1 regulation in beta-thalassemia/HbE. This provides novel insights into the phagocytic clearance of abnormal RBCs in beta-thalassemia/HbE.