Review
Cell & Tissue Engineering
Zijun Cai, Di Liu, Yuntao Yang, Wenqing Xie, Miao He, Dengjie Yu, Yuxiang Wu, Xiuhua Wang, Wenfeng Xiao, Yusheng Li
Summary: This article reviews the relationship between sarcopenia and stem cells, discussing the roles of satellite cells (SCs) and muscle-derived stem cells (MDSCs) in sarcopenia. Studies have shown that changes in the number and function of stem cells can trigger sarcopenia and have adverse effects on stem cells. A better understanding of the role of stem cells, especially SCs and MDSCs, in muscle, is important for developing novel therapeutic approaches to combat sarcopenia.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Mohamed Elashry, Mebrie Kinde, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: In this study, it was found that exposure to hypoxia enhanced the proliferation and motility of skeletal muscle-derived stem cells, promoted the differentiation of muscle and adipocytes, and improved the efficiency of myoblast differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Geriatrics & Gerontology
Rebecca E. Schmitt, Aneesha Dasgupta, Paige C. Arneson-Wissink, Srijani Datta, Alexandra M. Ducharme, Jason D. Doles
Summary: Up to half of sepsis survivors suffer from chronic critical illness (CCI), which includes multiple physical and mental disabilities that hinder their return to regular activities. A study has shown that muscle stem cells (MuSCs) and muscle regeneration play crucial roles in post-sepsis muscle recovery and that sepsis triggers morphological, functional, and transcriptional changes in MuSCs.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Review
Biochemistry & Molecular Biology
Korin Sahinyan, Felicia Lazure, Darren M. Blackburn, Vahab D. Soleimani
Summary: This review discusses the factors and effects of declining numbers and regenerative potential of muscle stem cells (MuSCs) during aging, and emphasizes the importance of understanding these changes for developing effective therapies for age-related muscle defects.
Article
Biochemistry & Molecular Biology
Yundong Peng, Jingjing Du, Stefan Gunther, Xinyue Guo, Shengpeng Wang, Andre Schneider, Li Zhu, Thomas Braun
Summary: Skeletal muscle stem cells, also known as satellite cells, play a crucial role in postnatal muscle growth and regeneration. This study reveals that Piezo1, a mechanosensitive ion channel, is essential for maintaining the quiescence and preventing senescence of muscle stem cells. Absence of Piezo1 leads to premature activation of these cells, impairs their proliferation and differentiation, and significantly hinders skeletal muscle regeneration. The inactivation of Piezo1 results in compensatory up-regulation of T-type voltage-gated Ca2+ channels, which increases the influx of Ca2+ and induces NOX4 expression through cPKC. The elevated expression of NOX4 in Piezo1-deficient cells leads to increased levels of reactive oxygen species (ROS) and DNA damage, causing P53-dependent cellular senescence and cell death. The study also highlights the importance of the P53/P21-axis in mediating the cellular defects caused by the absence of Piezo1. This research sheds light on the critical role of Piezo1-mediated mechano-signaling in maintaining the quiescence and preventing senescence of muscle stem cells, and suggests that reduced mechano-signaling during aging may contribute to the accumulation of senescent cells and the decline of muscle stem cell numbers in geriatric individuals.
Review
Biochemistry & Molecular Biology
Monika Zmojdzian, Krzysztof Jagla
Summary: The neuromuscular system, consisting of various components, requires interactions between them during development and regeneration to ensure muscle function. The interaction between muscle stem cells and motor neurons is crucial for building functional muscle.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell Biology
Hadi Boukhatmi
Summary: Experiments in Drosophila have been essential in understanding the molecular and cellular mechanisms regulating MuSCs, with a rich genetic toolbox available. The study revealed how AMPs in fruit flies are positioned, acquire identity, and are controlled by environmental signals. Recent identification of adult MuSCs in fruit flies with similar characteristics to vertebrates opens up new possibilities for muscle stem cell biology research.
Article
Geriatrics & Gerontology
Lei Liu, Xianlin Yue, Zewei Sun, William S. Hambright, Jianming Wei, Ying Li, Polina Matre, Yan Cui, Zhihui Wang, George Rodney, Johnny Huard, Paul D. Robbins, Xiaodong Mu
Summary: This study found that using senolytics in the muscles of patients with progeria can effectively remove senescent cells and improve the function of muscle progenitor/stem cells.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Cell & Tissue Engineering
Aiping Lu, Chieh Tseng, Ping Guo, Zhanguo Gao, Kaitlyn E. Whitney, Mikhail G. Kolonin, Johnny Huard
Summary: This study reveals that during aging, PDGFR beta lineage cells in skeletal muscle undergo fate-switching, participating in adipose and fibrotic tissue infiltration instead of functioning as muscle progenitor cells. The muscle regenerative process is also impaired in aged mice.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Camila F. Almeida, Marc Bitoun, Mariz Vainzof
Summary: Research on the KI-Dnm2(R465W/+) mouse model for AD-CNM showed impaired muscle regeneration with reduced number of Pax7-positive SCs and slower, less efficient myofiber formation, leading to failure to recover normal muscle mass 15 days after injury.
Article
Cell Biology
Pei Zhu, Noah X. Hamlish, Abhishek Vijay Thakkar, Adam W. T. Steffeck, Emily J. Rendleman, Nabiha H. Khan, Nathan J. Waldeck, Andrew W. DeVilbiss, Misty S. Martin-Sandoval, Thomas P. Mathews, Navdeep S. Chandel, Clara B. Peek
Summary: In this study, Zhu et al. discovered that the clock within adult muscle stem cells (MuSCs) plays a role in controlling muscle regeneration following acute ischemic injury. They observed that muscle repair capacity was higher during the active/wake period compared to the inactive/rest period in mice. Loss of the Bmal1 gene in MuSCs resulted in impaired muscle regeneration. The researchers demonstrated that Bmal1 loss led to reduced activation of MuSCs, impaired cell proliferation and myofiber differentiation, and premature activation of genes involved in muscle differentiation and epigenetic remodeling. Furthermore, they found that these effects could be restored by increasing cytosolic NAD(+). Overall, the MuSC clock is an important regulator of oxygen-dependent muscle cell fate and muscle repair.
GENES & DEVELOPMENT
(2022)
Review
Biology
Xin Fu, Qiang He, Yu Tao, Mengdi Wang, Wei Wang, Yalong Wang, Qing Cissy Yu, Fang Zhang, Xiaoyu Zhang, Ye-Guang Chen, Dong Gao, Ping Hu, Lijian Hui, Xiaoqun Wang, Yi Arial Zeng
Summary: Stem cells are crucial undifferentiated cells with the ability of self-renewal and differentiation, playing a significant role in organ and tissue development and repair. Tissue-specific stem cells hold powerful potential for regeneration and are promising for future research in tissue stem cell biology.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Yoshitaka Iso, Sayaka Usui, Hiroshi Suzuki
Summary: The aim of this study was to investigate the pro-angiogenic potential of skeletal muscle mesenchymal stem/stromal cells (mMSCs). The results showed that PDGFR-alpha positive mMSCs secreted VEGF and hepatocyte growth factor, inducing endothelial tube formation and promoting capillary growth. Epo stimulation enhanced phosphorylation and cellular proliferation in mMSCs, leading to improved perfusion recovery and capillary growth in the ischemic limbs.
Article
Biotechnology & Applied Microbiology
Rowoon Park, Jung Won Yoon, Jin-Ho Lee, Suck Won Hong, Jae Ho Kim
Summary: Researchers have developed an ultrathin cell-culture platform with potential-responsive nanoscale biointerfaces for monitoring mesenchymal stem cells (MSCs). The platform allows for highly ordered organization and spontaneous differentiation of MSCs, providing a robust strategy for directing stem cells and differentiation.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wenbin Liu, Tihui Wang, Wei Wang, Xingzuan Lin, Kailuo Xie
Summary: Salvia miltiorrhiza Bunge, a traditional Chinese medicine, has been widely used to treat muscle disorders. This study found that Tanshinone IIA (Tan IIA), the main active compound in Salvia miltiorrhiza Bunge, can promote the proliferation and differentiation ability of muscle stem cells (MuSCs) by activating MAPK and Akt signaling pathways. The results also showed that Tan IIA treatment improved muscle regeneration and muscle function recovery.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Editorial Material
Cell & Tissue Engineering
Alicia A. Cutler, Bradley B. Olwin
Article
Multidisciplinary Sciences
Katherine E. Gadek, Hong Wang, Monica N. Hall, Mitchell Sungello, Andrew Libby, Drew MacLaskey, Robert H. Eckel, Bradley B. Olwin
Article
Multidisciplinary Sciences
Bradley Pawlikowski, Nicole Dalla Betta, Tiffany Elston, Darian A. Williams, Bradley B. Olwin
SCIENTIFIC REPORTS
(2018)
Article
Clinical Neurology
Glen H. Nuckolls, Kathi Kinnett, Sudarshan Dayanidhi, Andrea A. Domenighetti, Tina Duong, Yetrib Hathout, Michael W. Lawlor, Sabrina S. M. Lee, S. Peter Magnusson, Craig M. McDonald, Elizabeth M. McNally, Natalie F. Miller, Bradley B. Olwin, Preeti Raghavan, Thomas J. Roberts, Seward B. Rutkove, John F. Sarwark, Claudia R. Senesac, Leslie F. Vogel, Glenn A. Walter, Rebecca J. Willcocks, William Z. Rymer, Richard L. Lieber
Review
Biochemistry & Molecular Biology
Kaitlin Weskamp, Bradley B. Olwin, Roy Parker
Summary: This article discusses the overlapping post-transcriptional regulatory pathways that mediate muscle development, the disruption of these pathways in neuromuscular disorders, and the potential of RNA-mediated therapies in treating these diseases.
TRENDS IN MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Jason S. Silver, K. Arda Gunay, Alicia A. Cutler, Thomas O. Vogler, Tobin E. Brown, Bradley T. Pawlikowski, Olivia J. Bednarski, Kendra L. Bannister, Cameron J. Rogowski, Austin G. Mckay, Frank W. DelRio, Bradley B. Olwin, Kristi S. Anseth
Summary: Elevated muscle stiffness enhances migration and proliferation of MuSCs by localizing YAP and TAZ to the nucleus. Ablating YAP and TAZ induces MuSC quiescence after injury and prevents myofiber hypertrophy.
Review
Nutrition & Dietetics
Josiane Joseph, Jason D. Doles
Summary: Chronic disease patients may suffer a loss of lean muscle mass due to disruptions in satellite cell metabolism. This can impair muscle regeneration and exacerbate muscle wasting. Understanding how metabolic disruptions in satellite cells contribute to wasting is important for potential therapeutic interventions.
NUTRITION & METABOLISM
(2021)
Article
Engineering, Biomedical
K. Arda Gunay, Jason S. Silver, Tze-Ling Chang, Olivia J. Bednarski, Kendra L. Bannister, Cameron J. Rogowski, Bradley B. Olwin, Kristi S. Anseth
Summary: The study demonstrates that the chaperone protein BAG3 is crucial in mediating dynamic stiffness changes in skeletal muscle tissue, connecting mechanical alterations to intracellular signals and influencing muscle progenitor cell morphology and differentiation.
Article
Oncology
Paige C. Arneson-Wissink, Jason D. Doles
Summary: Skeletal muscle wasting is a key driver of negative clinical outcomes and is associated with a range of pathologies including cancer. Cancer cachexia is a multi-factorial syndrome that includes skeletal muscle wasting, but remains understudied. Research suggests that amino acid metabolic disruption is linked to myoblast defects induced by cancer cell conditioned media.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alicia A. Cutler, Bradley Pawlikowski, Joshua R. Wheeler, Nicole Dalla Betta, Tiffany Elston, Rebecca O'Rourke, Kenneth Jones, Bradley B. Olwin
Summary: Skeletal muscle stem cells, or satellite cells (SCs), play a crucial role in muscle regeneration and maintenance. Recent research has shown that most SCs reacquire quiescence 5-10 days after muscle injury, following differentiation and fusion processes. Single-cell sequencing has identified SCs reacquiring quiescence and revealed the influence of noncell autonomous signaling networks on SC fate decisions during regeneration. SC transplantation experiments have confirmed the impact of the regenerative environment on SC fate.
Article
Biology
Joshua R. Wheeler, Oscar N. Whitney, Thomas O. Vogler, Eric D. Nguyen, Bradley Pawlikowski, Evan Lester, Alicia Cutler, Tiffany Elston, Nicole Dalla Betta, Kevin R. Parker, Kathryn E. Yost, Hannes Vogel, Thomas A. Rando, Howard Y. Chang, Aaron M. Johnson, Roy Parker, Bradley B. Olwin
Summary: This research reveals that the timing of RNA-binding protein (RBPs) expression can specify the fate transitions of muscle stem cells, playing a crucial role in skeletal muscle regeneration.
Article
Cell & Tissue Engineering
Rebecca E. Schmitt, Douglas Y. Smith, Dong Seong Cho, Lindsey A. Kirkeby, Zachary T. Resch, Teerin Liewluck, Zhiyv Niu, Margherita Milone, Jason D. Doles
Summary: This study investigated the underlying mechanisms of GNE myopathy using a patient-derived induced pluripotent stem cell (iPSC) model. The findings revealed defects in myogenic progression and alterations in stress and autophagy signaling in GNE myopathy patient-derived iPSCs. Treatment with an autophagy activator improved myogenic differentiation in the patient-derived iPSCs.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Geriatrics & Gerontology
Rebecca E. Schmitt, Aneesha Dasgupta, Paige C. Arneson-Wissink, Srijani Datta, Alexandra M. Ducharme, Jason D. Doles
Summary: Up to half of sepsis survivors suffer from chronic critical illness (CCI), which includes multiple physical and mental disabilities that hinder their return to regular activities. A study has shown that muscle stem cells (MuSCs) and muscle regeneration play crucial roles in post-sepsis muscle recovery and that sepsis triggers morphological, functional, and transcriptional changes in MuSCs.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Biology
Dong Seong Cho, Bolim Lee, Jason D. Doles
LIFE SCIENCE ALLIANCE
(2019)
Article
Neurosciences
Alicia A. Cutler, Theodore Eugene Ewachiw, Giulia A. Corbet, Roy Parker, Brad B. Olwin
JOURNAL OF EXPERIMENTAL NEUROSCIENCE
(2019)
