Article
Clinical Neurology
Rui Ryan Yang, Kay Ka-Wai Li, Zhen-Yu Zhang, Aden Ka-Yin Chan, Wei-Wei Wang, Danny Tat-Ming Chan, Wen-Cai Li, Xian-Zhi Liu, Fang-Cheng Li, Hong Chen, Ho-Keung Ng, Ying Mao, Zhi-Feng Shi
Summary: The diagnostic role of Isocitrate Dehydrogenase (IDH) mutation status in adult lower grade astrocytomas is significant, potentially further stratified by MMR genes. Our study suggests the potential clinical relevance of MMR genes in IDH-mutant lower grade astrocytomas without certain genetic alterations like PDGFRA, CDK4, and CDKN2A.
CLINICAL NEUROLOGY AND NEUROSURGERY
(2021)
Article
Oncology
Ibrahim Halil Sahin, Subir Goyal, Yoanna Pumpalova, Mohamad B. Sonbol, Satya Das, Sigurdis Haraldsdottir, Daniel Ahn, Kristen K. Ciombor, Zhengjia Chen, Amber Draper, Jordan Berlin, Tanios Bekaii-Saab, Gregory B. Lesinski, Bassel F. El-Rayes, Christina Wu
Summary: Liver metastasis, MLH1/PMS2 loss, BRAF V600E mutation, and age over 65 may predict the duration of response to immune checkpoint inhibitors in patients with MMR-D CRC.
Article
Oncology
Ellis L. Eikenboom, Helena C. van Doorn, Winand N. M. Dinjens, Hendrikus J. Dubbink, Willemina R. R. Geurts-Giele, Manon C. W. Spaander, Carli M. J. Tops, Anja Wagner, Anne Goverde
Summary: In this study, outcomes of gynecological surveillance and risk-reducing surgery in 164 Lynch syndrome carriers were assessed, revealing variability in surveillance timing and questioning the benefits of surveillance for endometrial cancers found in early stage.
Article
Chemistry, Analytical
Damaris-Cristina Gheorghe, Raluca-Ioana Stefan-van Staden, Florina Pogacean, Stela Pruneanu
Summary: Two stochastic sensors modified with maltodextrin-doped graphene were successfully developed for the molecular identification and quantification of MLH1, MSH2, MSH6, PMS2, and KRAS in biological samples. These sensors exhibit extremely low limits of determination and wide linear concentration ranges, allowing for the accurate detection and quantification of target molecules in healthy individuals and cancer patients. The recoveries of the target molecules in different biological samples exceeded 98% with high precision.
Article
Biology
Adi Amar-Schwartz, Vered Ben Hur, Amina Jbara, Yuval Cohen, Georgina D. Barnabas, Eliran Arbib, Zahava Siegfried, Bayan Mashahreh, Fouad Hassouna, Asaf Shilo, Mohammad Abu-Odeh, Michael Berger, Reuven Wiener, Rami Aqeilan, Tamar Geiger, Rotem Karni
Summary: In this study, we discovered a novel role for S6K1 in regulating DNA repair through the coordinated regulation of the cell cycle, homologous recombination (HR) DNA repair (HRR) and mismatch DNA repair (MMR) mechanisms. Moreover, abnormal function of S6K1 is associated with drug resistance and poor survival in breast cancer patients.
Article
Biochemistry & Molecular Biology
Katja Kratz, Mariela Artola-Boran, Saho Kobayashi-Era, Gene Koh, Goncalo Oliveira, Shunsuke Kobayashi, Andreia Oliveira, Xueqing Zou, Julia Richter, Masataka Tsuda, Hiroyuki Sasanuma, Shunichi Takeda, Joanna Loizou, Alessandro A. Sartori, Serena Nik-Zainal, Josef Jiricny
Summary: A study revealed a new exonuclease, FAN1, which can efficiently substitute for EXO1 in the mismatch repair process, with its functional complementation modulated by its interaction with MLH1. Loss of FAN1 exacerbates the mutational profile of EXO1-deficient cells, suggesting redundant action of these two nucleases in the same antimutagenic pathway.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Oncology
Ha Kyun Chang, Young Hoon Park, Jung-A Choi, Jeong Won Kim, Jisup Kim, Hyo Sun Kim, Hae Nam Lee, Hanbyoul Cho, Joon-Yong Chung, Jae-Hoon Kim
Summary: The expression of Nectin-4 is significantly increased in endometrial cancer and can be used as a diagnostic marker. High Nectin-4 expression is associated with DNA mismatch repair protein deficiency and may predict short progression-free survival. This study provides clues for selecting patients for adjuvant therapy.
Article
Oncology
Yoland Antill, Daniel D. Buchanan, Clare L. Scott
Summary: Endometrial cancer patients with Lynch gene or POLE/POLD1 gene mutations are more likely to respond well to immune therapy, while those with silencing of the MLH1 gene may not be as responsive. Combination therapies may be important to consider for these patients.
Article
Oncology
Soyoun Rachel Kim, Alicia Tone, Raymond H. Kim, Matthew Cesari, Blaise A. Clarke, Lua Eiriksson, Tae Hart, Melyssa Aronson, Spring Holter, Alice Lytwyn, Katherine Lajkosz, Leslie Oldfield, Steven Gallinger, Marcus Q. Bernardini, Amit M. Oza, Bojana Djordjevic, Jordan Lerner-Ellis, Emily Van de Laar, Danielle Vicus, Trevor Pugh, Aaron Pollett, Sarah E. Ferguson
Summary: The study compared the clinical characteristics, survival, and recurrence patterns between MMRd and MMRi EEC. The results showed that MMRd tumors have more aggressive features, lower recurrence-free survival, and are more likely to recur in retroperitoneal lymph nodes. MLH1 methylated tumors had the worst prognostic features and survival outcomes.
GYNECOLOGIC ONCOLOGY
(2021)
Article
Genetics & Heredity
Matthew L. DuPrie, Tatiana Palacio, Felipe A. Calil, Richard D. Kolodner, Christopher D. Putnam
Summary: In eukaryotic DNA mismatch repair, Mlh1 is recruited to the repair site through interaction with the subunits of Msh2-Msh6, rather than with the subunits of Pms1.
Article
Oncology
Zachary P. Schwartz, Andrew J. Li, Christine S. Walsh, B. J. Rimel, Monica M. Alvarado, Scott E. Lentz, Ilana Cass
Summary: This study aimed to investigate the practice patterns and frequency of occult gynecologic cancers in women with pathogenic variants of non-BRCA and Lynch syndrome-associated ovarian cancer susceptibility genes who underwent risk-reducing bilateral salpingo-oophorectomy (RRSO). The results showed no cases of occult ovarian or fallopian tube cancer in both groups, but 2 patients (3%) in the Lynch group had occult endometrial cancer. The use of hormone replacement therapy (HRT) was rare despite reported menopausal symptoms.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Obstetrics & Gynecology
Brett Kurpiel, Martha S. Thomas, Mansoor Mubeen, Kari L. Ring, Susan C. Modesitt, Christopher A. Moskaluk, Anne M. Mills
Summary: MLH1 hypermethylation is the most common cause of mismatch repair deficiency in endometrial carcinoma, but somatic and germline mutations in MLH1 can also occur. Some patients with hypermethylated tumors may also have germline variants of unknown significance in other mismatch repair genes and genes involved in hereditary cancer syndromes, but their clinical relevance is unclear.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
(2022)
Article
Obstetrics & Gynecology
Brett Kurpiel, Martha S. Thomas, Mansoor Mubeen, Kari L. Ring, Susan C. Modesitt, Christopher A. Moskaluk, Anne M. Mills
Summary: Epigenetic hypermethylation of the MLH1 promoter is the most common cause of mismatch repair deficiency in endometrial carcinoma. MLH1 hypermethylation accounts for the vast majority of MLH1/PMS2-deficient cancers, but somatic and germline mutations can also occur.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
(2022)
Article
Genetics & Heredity
Ying L. Liu, Karen A. Cadoo, Anna Maio, Zalak Patel, Yelena Kemel, Erin Salo-Mullen, Amanda Catchings, Megha Ranganathan, Sarah Kane, Robert Soslow, Ozge Ceyhan-Birsoy, Diana Mandelker, Maria I. Carlo, Michael F. Walsh, Jinru Shia, Arnold J. Markowitz, Kenneth Of, Alicia Latham
Summary: This study aimed to characterize MSH6/PMS2-associated mismatch repair-deficient (MMR-D)/microsatellite instability-high (MSI-H) tumors. It found that patients with germline MSH6/PMS2 pathogenic/likely pathogenic variants are at risk for a broad spectrum of cancers, with CRC and EC being the most common. The study also highlighted the occurrence of MMR-D/MSI-H CRC and EC at younger ages.
GENETICS IN MEDICINE
(2022)
Article
Oncology
Thiago Pereira Diniz, Jacqueline Nunes Menezes, Bruna Tirapelli Goncalves, Carlos Chaves Faloppa, Henrique Mantoan, Lillian Yuri Kumagai, Levon Badiglian-Filho, Graziele Bovolim, Andrea Paiva Gadelha Guimaraes, Louise De Brot, Glauco Baiocchi
Summary: This study evaluated the relationship between mismatch repair (MMR) status and the risk of lymph node metastasis in endometrial cancer. MMR deficiency was found to be correlated with higher grade and lymphovascular space invasion, and it was an independent risk factor for lymph node metastasis. Furthermore, MMR status may help in selecting patients who can avoid systematic lymphadenectomy.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Obstetrics & Gynecology
Linyuan Wang, Anna Piskorz, Tjalling Bosse, Mercedes Jimenez-Linan, Brian Rous, C. Blake Gilks, James D. Brenton, Naveena Singh, Martin Kobel
Summary: PTEN plays a central role in the pathogenesis of endometrial carcinoma. PTEN IHC shows moderate accuracy in predicting loss of function PTEN mutations, and can be used for diagnostic purposes.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
(2022)
Article
Oncology
Julie M. Cunningham, Stacey J. Winham, Chen Wang, Britta Weiglt, Zhuxuan Fu, Sebastian M. Armasu, Bryan M. McCauley, Alison H. Brand, Yoke-Eng Chiew, Esther Elishaev, Charlie Gourley, Catherine J. Kennedy, Angela Laslavic, Jenny Lester, Anna Piskorz, Magdalena Sekowska, James D. Brenton, Michael Churchman, Anna DeFazio, Ronny Drapkin, Kevin M. Elias, David G. Huntsman, Beth Y. Karlan, Martin Kobel, Jason Konner, Kate Lawrenson, Elli Papaemmanuil, Kelly L. Bolton, Francesmary Modugno, Ellen L. Goode
Summary: This study investigated the role of DNA methylation in ovarian clear cell carcinoma (OCCC). Two clusters that correlated with clinical features were identified. Tumors in Cluster 1 showed TP53 mutation and abnormal p53 expression, while tumors in Cluster 2 showed aneuploidy and ARID1A/PIK3CA mutation. Differentially expressed genes were enriched for immune-related pathways.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2022)
Article
Oncology
Sarah Nicole Hamilton, Anna Tinker, Janice Kwon, Peter Lim, Iwa Kong, Sona Sihra, Martin Koebel, Cheng Han Lee
Summary: This study evaluates the disease characteristics, treatment, and survival outcomes of patients with undifferentiated and dedifferentiated endometrial carcinoma treated at BC Cancer. The results show that patients with stage I/II have good survival outcomes, and adjuvant chemotherapy and radiotherapy can improve disease-free survival. The incidence of abnormal profiles is high in patients who undergo MMR testing.
JOURNAL OF GYNECOLOGIC ONCOLOGY
(2022)
Article
Oncology
Karolin Heinze, Tayyebeh M. Nazeran, Sandra Lee, Pauline Kramer, Evan S. Cairns, Derek S. Chiu, Samuel C. Y. Leung, Eun Young Kang, Nicola S. Meagher, Catherine J. Kennedy, Jessica Boros, Friedrich Kommoss, Hans-Walter Vollert, Florian Heitze, Andreas du Bois, Philipp Harter, Marcel Grube, Bernhard Kraemer, Annette Staebler, Felix K. F. Kommoss, Sabine Heublein, Hans-Peter Sinn, Naveena Singh, Angela Laslavic, Esther Elishaev, Alex Olawaiye, Kirsten Moysich, Francesmary Modugno, Raghwa Sharma, Alison H. Brand, Paul R. Harnett, Anna DeFazio, Renee T. Fortner, Jan Lubinski, Marcin Lener, Aleksandra Toloczko-Grabarek, Cezary Cybulski, Helena Gronwald, Jacek Gronwald, Penny Coulson, Mona A. El-Bahrawy, Michael E. Jones, Minouk J. Schoemaker, Anthony J. Swerdlow, Kylie L. Gorringe, Ian Campbell, Linda Cook, Simon A. Gayther, Michael E. Carney, Yurii B. Shvetsov, Brenda Y. Hernandez, Lynne R. Wilkens, Marc T. Goodman, Constantina Mateoiu, Anna Linder, Karin Sundfeldt, Linda E. Kelemen, Aleksandra Gentry-Maharaj, Martin Widschwendter, Usha Menon, Kelly L. Bolton, Jennifer Alsop, Mitul Shah, Mercedes Jimenez-Linan, Paul D. P. Pharoah, James D. Brenton, Kara L. Cushing-Haugen, Holly R. Harris, Jennifer A. Doherty, Blake Gilks, Prafull Ghatage, David G. Huntsman, Gregg S. Nelson, Anna Tinker, Cheng-Han Lee, Ellen L. Goode, Brad H. Nelson, Susan J. Ramus, Stefan Kommoss, Aline Talhouk, Martin Kobel, Michael S. Anglesio
Summary: ARID1A (BAF250a) is an important component of the SWI/SNF chromatin modifying complex and plays a suppressor role in ovarian cancer. This study investigated the relationship between ARID1A loss/mutation and clinical features, outcomes, immune response, and DNA mismatch repair deficiency in ovarian carcinomas. The findings showed that ARID1A loss was more prevalent in clear cell ovarian carcinomas, and in endometrioid ovarian carcinomas, it was associated with younger age, DNA mismatch repair deficiency, and the presence of CD8(+) tumor-infiltrating lymphocytes. The study provides important insights into the prevalence and prognostic significance of ARID1A loss-of-function mutations in endometriosis-associated ovarian cancers.
JOURNAL OF PATHOLOGY
(2022)
Article
Pathology
Eun Young Kang, Joshua Millstein, Gordana Popovic, Nicola S. Meagher, Adelyn Bolithon, Aline Talhouk, Derek S. Chiu, Michael S. Anglesio, Betty Leung, Katrina Tang, Neil Lambie, Marina Pavanello, Annalyn Da-Anoy, Diether Lambrechts, Liselore Loverix, Siel Olbrecht, Christiani Bisinotto, Jesus Garcia-Donas, Sergio Ruiz-Llorente, Monica Yague-Fernandez, Robert P. Edwards, Esther Elishaev, Alexander Olawaiye, Sarah Taylor, Beyhan Ataseven, Andreas du Bois, Philipp Harter, Jenny Lester, Claus K. Hogdall, Sebastian M. Armasu, Yajue Huang, Robert A. Vierkant, Chen Wang, Stacey J. Winham, Sabine Heublein, Felix K. F. Kommoss, Daniel W. Cramer, Naoko Sasamoto, Lilian Van-Wagensveld, Maria Lycke, Constantina Mateoiu, Janine Joseph, Malcolm C. Pike, Kunle Odunsi, Chiu-Chen Tseng, Celeste L. Pearce, Sanela Bilic, Thomas P. Conrads, Arndt Hartmann, Alexander Hein, Michael E. Jones, Yee Leung, Matthias W. Beckmann, Matthias Ruebner, Minouk J. Schoemaker, Kathryn L. Terry, Mona A. El-Bahrawy, Penny Coulson, John L. Etter, Katherine LaVigne-Mager, Juergen Andress, Marcel Grube, Anna Fischer, Nina Neudeck, Greg Robertson, Rhonda Farrell, Ellen Barlow, Carmel Quinn, Anusha Hettiaratchi, Yovanni Casablanca, Ramona Erber, Colin J. R. Stewart, Adeline Tan, Yu Yu, Jessica Boros, Alison H. Brand, Paul R. Harnett, Catherine J. Kennedy, Nikilyn Nevins, Terry Morgan, Peter A. Fasching, Ignace Vergote, Anthony J. Swerdlow, Francisco J. Candido Dos Reis, G. Larry Maxwell, Susan L. Neuhausen, Arantzazu Barquin-Garcia, Francesmary Modugno, Kirsten B. Moysich, Philip J. Crowe, Akira Hirasawa, Florian Heitz, Beth Y. Karlan, Ellen L. Goode, Peter Sinn, Hugo M. Horlings, Estrid Hogdall, Karin Sundfeldt, Stefan Kommoss, Annette Staebler, Anna H. Wu, Paul A. Cohen, Anna DeFazio, Cheng-Han Lee, Helen Steed, Nhu D. Le, Simon A. Gayther, Kate Lawrenson, Paul D. P. Pharoah, Gottfried Konecny, Linda S. Cook, Susan J. Ramus, Linda E. Kelemen, Martin Kobel
Summary: MCM3 mRNA and protein expression levels are associated with survival in patients with tubo-ovarian high-grade serous carcinomas (HGSC), showing a correlation between high MCM3 expression and longer overall survival.
Review
Oncology
Martin Kobel, Eun Young Kang
Summary: Research has shown that ovarian carcinomas consist of multiple distinct histologic types and molecular subtypes, representing different diseases. By grouping tumors based on molecular similarities, research and future patient management can be facilitated. Utilizing immunohistochemical markers and histotype-specific oncogenic alterations can confirm morphological diagnoses for different histotypes.
Article
Pathology
Eun Young Kang, Nicholas J. P. Wiebe, Christa Aubrey, Cheng-Han Lee, Michael S. Anglesio, Derek Tilley, Prafull Ghatage, Gregg S. Nelson, Sandra Lee, Martin Kobel
Summary: The study aimed to test the sensitivity of tumor morphology in capturing p53 abnormal cases and model the impact of p53 abnormality on ESGO risk stratification. The results showed that predicting p53 abnormality using nuclear features is accurate, and in a small percentage of cases, p53 abnormality can lead to changes in ESGO risk group classification.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Pathology
Felix K. F. Kommoss, Sarah Chiang, Martin Kobel, Christian Koelsche, Kenneth Tou-En Chang, Julie A. Irving, Brendan Dickson, Sakinah Thiryayi, Marjan Rouzbahman, Golnar Rasty, Andreas von Deimling, Cheng-Han Lee, Gulisa Turashvili
Summary: The study aims to clarify the classification of endometrial stromal sarcomas (ESS) with variant alterations involving BCOR or BCORL1 by describing their clinicopathologic and molecular features. The results indicate that ESS with BCOR internal tandem duplication and variant BCOR and BCORL1 rearrangements clinically and molecularly resemble conventional high-grade endometrial stromal sarcomas.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2022)
Article
Cell Biology
Basile Tessier-Cloutier, Eun-Young Kang, Deepu Alex, Colin J. R. Stewart, W. Glenn McCluggage, Martin Kobel, Cheng-Han Lee
Summary: This study found that neuroendocrine marker expression in SWI/SNF-deficient DDECs/UDECs is typically focal, while NECs typically express two or more neuroendocrine markers. Immunohistochemistry can be used to differentiate between DDEC/UDEC and NEC.
Article
Oncology
Felix K. F. Kommoss, Basile Tessier-Cloutier, Leora Witkowski, Erna Forgo, Christian Koelsche, Martin Kobel, William D. Foulkes, Cheng-Han Lee, David L. Kolin, Andreas von Deimling, Brooke E. Howitt
Summary: SWI/SNF complex deficiency is common in various cancers, especially in the gynecologic tract. Despite their similar morphology, UDEC, SDUS, and SCCOHT have distinct DNA methylation signatures. Our study highlights the importance of cellular context in determining the epigenetic landscape and suggests that methylation profiling may be a useful diagnostic tool in undifferentiated, SWI/SNF-deficient cancers.
JOURNAL OF PATHOLOGY
(2022)
Article
Oncology
Kelly L. Bolton, Denise Chen, Rosario Corona de la Fuente, Zhuxuan Fu, Rajmohan Murali, Martin Kobel, Yanis Tazi, Julie M. Cunningham, Irenaeus C. C. Chan, Brian J. Wiley, Lea A. Moukarzel, Stacey J. Winham, Sebastian M. Armasu, Jenny Lester, Esther Elishaev, Angela Laslavic, Catherine J. Kennedy, Anna Piskorz, Magdalena Sekowska, Alison H. Brand, Yoke-Eng Chiew, Paul Pharoah, Kevin M. Elias, Ronny Drapkin, Michael Churchman, Charlie Gourley, Ann DeFazio, Beth Karlan, James D. Brenton, Britta Weigelt, Michael S. Anglesio, David Huntsman, Simon Gayther, Jason Konner, Francesmary Modugno, Kate Lawrenson, Ellen L. Goode, Elli Papaemmanuil
Summary: This study identifies two distinct molecular subclasses of clear cell ovarian carcinoma (CCOC) with different clinical presentations and outcomes. Tumors with ARID1A mutations are associated with platinum resistance, while tumors with TP53 mutations are associated with extracellular matrix organization and mesenchymal differentiation. TP53-mutated tumors are more likely to be advanced-stage, without a history of endometriosis, and have poorer survival.
CLINICAL CANCER RESEARCH
(2022)
Article
Obstetrics & Gynecology
Teresa H. Praetorius, Anna Leonova, Vivian Lac, Janine Senz, Basile Tessier-Cloutier, Tayyebeh M. Nazeran, Martin Kobel, Marcel Grube, Bernhard Kraemer, Paul J. Yong, Stefan Kommoss, Michael S. Anglesio
Summary: This study investigated the heterogeneity of somatic cancer-driver mutations within patients and across different types of endometriosis. The results showed that identical mutations were found across distinct lesions, indicating clonality in endometriosis. Additionally, endometriomas exhibited higher mutational complexity, with functionally redundant driver mutations within the same gene. The study suggests that the current anatomically defined classification of endometriosis does not fully recognize the etiology of the disease, and a novel classification considering genomic and other molecular features should be considered.
FERTILITY AND STERILITY
(2022)
Article
Oncology
Dane Cheasley, Marta Llaurado Fernandez, Martin Kobel, Hannah Kim, Amy Dawson, Joshua Hoenisch, Madison Bittner, Derek S. Chiu, Aline Talhouk, C. Blake Gilks, Madawa W. Jayawardana, Kathleen Pishas, Anne-Marie Mes-Masson, Diane Provencher, Abhimanyu Nigam, Neville F. Hacker, Kylie L. Gorringe, Ian G. Campbell, Mark S. Carey
Summary: This study investigates the genomic profiles associated with estrogen receptor (ER) and progesterone receptor (PR) expression patterns in low-grade serous ovarian carcinoma (LGSOC). The results show that PR-low tumors have a higher fraction of the genome altered by copy number changes compared to PR-high tumors. Higher expression of ER and PR is associated with improved overall survival.
NPJ PRECISION ONCOLOGY
(2022)
Article
Oncology
Jennifer X. Ji, Dawn R. Cochrane, Gian Luca Negri, Shane Colborne, Sandra E. Spencer Miko, Lynn N. Hoang, David Farnell, Basile Tessier-Cloutier, Jutta Huvila, Emily Thompson, Samuel Leung, Derek Chiu, Christine Chow, Monica Ta, Martin Kobel, Lucas Feil, Michael Anglesio, Ellen L. Goode, Kelly Bolton, Gregg B. Morin, David G. Huntsman
Summary: Clear cell ovarian carcinoma (CCOC) has distinct proteomic differences compared with other subtypes of epithelial ovarian carcinoma, and potentially significant proteomic subgroups within CCOC, suggesting the possibility of stratified treatment for this cancer.
JOURNAL OF PATHOLOGY
(2022)
Article
Anatomy & Morphology
Maire A. Duggan, Qiuli Duan, Ruth M. Pfeiffer, Mary Anne Brett, Sandra Lee, Mustapha Abubakar, Martin Kobel, Monica Rodriguez, Aylin Sar
Summary: This study aimed to compare the diagnoses of the International Endocervical adenocarcinoma Criteria and Classification (IECC) algorithm with H&E-based IECC histotyping and validated the use of random forest (RF) classification for more accurate discrimination. The results showed that the IECC algorithm performed poorly in histotyping, while the RF algorithm showed favorable results in distinguishing between HPVA and NHPV.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2022)