Bio-synthesis of Silver Nanoparticles Using Water Extract of Satureja hortensis L and Evaluation of the Antibacterial Properties

Background: Chemical methods for reducing the silver ions may have incompatible effects in medical applications, so the synthesis of nanoparticles using microorganisms and plants extract may help to solve this problem. Therefore biological approaches for synthesis of nanoparticles are better than chemical and physical procedures because of low energy and time expenditure, non toxic solvents and non dangerous material for environment. Methods: Bio-synthesis of silver nanoparticles (Ag NPs) using water extract of Satureja hortensis L is carried out successfully at room temperature. Results: TEM study showed that mean diameter and standard deviation for the formation of silver nanoparticles were 15 +/- 7.402 nm. The antibacterial effects of silver nanoparticles on Gram positive and Gram negative bacteria were also performed. Conclusion: It can be concluded that silver nanoparticles were synthesized with an average size of 15 +/- 7.402 nm and spherical in shape, using the water extract of Satureja hortensis L at room temperature. silver nanoparticles by UV-Visible, SEM, TEM and XRD were characterized. Synthesis of silver nanoparticles using plant like Satureja hortensis L is a better alternative to chemical synthesis. The green synthesis of nanoparticles is an eco-friendly method because of the usage of natural products. Also study found that the Ag/Satureja hortensis L shows antibacterial activity on both Gram positive and Gram negative bacteria.