期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 103, 期 -, 页码 286-293出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.05.060
关键词
Mannogalactofucans; Antiviral activity; Toll-like receptor 2
资金
- Gyeonggi-do GRRC Program of the Catholic University of Korea [2016-B01]
- Research Fund of The Catholic University of Korea
Low-molecular-weight mannogalactofucans (LMMGFs, <4000 g/mol) were prepared by the enzymatic degradation of Undaria pinnatifida sporophyll galactofucan (MF) and evaluated or their antiviral activities and underlying action mechanisms against herpes simplex virus type 1 (HSV-1). The 50% inhibitory concentrations (IC50) of LMMGFs and MF were 2.64 and 2.42 mu g/mL, respectively. LMMGFs inhibited the viral entry on the host cell surface and also exhibited inhibitory activity directly against viral particles, as observed in a virucidal assay. LMMGFs dose-dependently enhanced the mRNA expression of Toll-like receptor 2 (TLR2) and stimulated the phosphorylation of Akt and JNK in Vero cells. These results clearly demonstrated that LMMGFs use TLR2 as their receptor, preventing HSV-1 infection on the host cell surface and antagonizing viral adsorption via TLR2 pathway activation in Vero cells. (C) 2017 Elsevier B.V. All rights reserved.
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