4.7 Article

Deciphering the interaction of bovine heart cystatin with ZnO nanoparticles: Spectroscopic and thermodynamic approach

期刊

出版社

ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.10.095

关键词

ZnO-nanoparticles; Buffalo heart cystatin; Molten globule; XRD; TEM; SEM

资金

  1. University Grants Commission (UGC), Government of India, MAN-SRF

向作者/读者索取更多资源

ZnO-NPs have been widely used in biomedical fields such as therapeutics, cellular imaging, and drug delivery. However, the risk of exposure of nanoparticles to the biological system is not well understood. Nanoparticle-protein interaction is pivotal to understand their biological behavior and predict nanoparticle toxicity that is crucial for its safer applications. In the present study zinc oxide nanopartides (ZnO-NPs) were synthesized and subjected to interact with buffalo heart cystatin (BHC), purified from buffalo heart, to assess the effect(s) of ZnO-NPs on the structure and function of BHC. In vitro toxicity assessments revealed that BHC, upon interaction with ZnO-NPs, led to the altered protein conformation and perturbed function. A decrease in the anti-papain activity of BHC was observed. Spectroscopic studies demonstrated that formation of BHC-ZnO-NPs complex accompanied by structural changes in BHC along with a significant decrease in its a-helical content. ITC determined the thermodynamic parameters of binding between ZnO-NPs and BHC quantitatively. Increased surface hydrophobicity (change in the tertiary structure) was observed by ANS fluorescence that demonstrated the formation of molten globular intermediates that were found to be stable without any signs of aggregation as depicted by ThT fluorescence. TEM images gave the physical evidence of the formation of ZnO-NPs-BHC corona. (C) 2016 Published by Elsevier B.V.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

Review Biochemistry & Molecular Biology

Mammalian cystatin and protagonists in brain diseases

Fakhra Amin, Mohd Shahnawaz Khan, Bilqees Bano

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2020)

Article Biochemistry & Molecular Biology

Deciphering the Nature of Caffeic Acid to Inhibit the HSA Aggregation Induced by Glyoxal

Waseem Feeroze Bhat, Azaj Ahmed, Shabeena Abbass, Mohammad Afsar, Bilqees Bano, Akbar Masood

PROTEIN AND PEPTIDE LETTERS (2020)

Article Biochemistry & Molecular Biology

Amyloid aggregation and secondary structure changes of liver cystatin: Acidic denaturation and TFE induced studies

Faisal Mustafa Mir, Bilqees Bano

Summary: This study demonstrates that the conformation, activity, and fibril formation of the cysteine protease inhibitor from buffalo liver are affected by the presence of TFE under low pH conditions. TFE stabilizes the acid-induced intermediates and accelerates fibril formation, highlighting the significant impact of solvent conditions on the tendency of liver cystatin to produce fibrils and aggregation.

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2022)

Article Materials Science, Ceramics

Enhanced thermoelectric properties of tungsten oxide-reduced graphene oxide nanocomposites

Amish Kumar Gautam, Mohd Faraz, Neeraj Khare

Summary: This article synthesized tungsten oxide nanoparticles and tungsten oxide-reduced graphene oxide nanocomposites, and studied their thermoelectric properties. The results showed that the thermoelectric material figure of merit of WO3-rGO nanocomposites increased by 8.8 times at 313K, with significantly enhanced electrical conductivity and reduced thermal conductivity compared to WO3 nanoparticles.

CERAMICS INTERNATIONAL (2021)

Article Chemistry, Physical

A progressive strategy for harvesting mechanical energy using flexible PVDF-rGO-MoS2 nanocomposites film-based piezoelectric nanogenerator

Mohd Faraz, Huidrom Hemojit Singh, Neeraj Khare

Summary: Flexible thin films of PVDF, PVDF-MoS2, and PVDF-rGO-MoS2 were prepared by polymer solution casting method, and their piezoelectric properties were explored. These films exhibited significantly improved generated voltage when subjected to vibration, providing a potential alternative energy solution for low powered electronic devices.

JOURNAL OF ALLOYS AND COMPOUNDS (2022)

Article Biochemistry & Molecular Biology

Glyoxal induced glycative insult suffered by immunoglobulin G and fibrinogen proteins: A comparative physicochemical characterization to reveal structural perturbations

Sultan Alouffi, Uzma Shahab, Saif Khan, Mahvish Khan, Afreen Khanam, Rihab Akasha, S. D. Shahanawaz, Hussain Arif, Iram Khan Tahir, Shahnawaz Rehman, Saheem Ahmad

Summary: Glycation of proteins can lead to structural alteration, functional deprivation, and generation of advanced glycation end products (AGEs). Reactive oxygen species (ROS) generated during glucose autoxidation induce glycoxidation of glycated proteins, resulting in the formation of aldehyde and/or ketone groups containing dicarbonyls or reactive carbonyl species (RCS). RCS further react non-enzymatically, exacerbating oxidative, carbonyl, and glycative stress in the physiological system. This study focuses on the glycoxidative damage suffered by immunoglobulin G (IgG) and fibrinogen proteins induced by glyoxal (GO) and explores the resulting structural alterations and quantifies various biochemical markers. The results suggest that AGEs formation during GO-mediated glycation of IgG and fibrinogen could disrupt normal physiology and play a significant role in the pathogenesis of diabetes-associated secondary complications.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Article Chemistry, Inorganic & Nuclear

Visible light activated V2O5/rGO nanocomposite for enhanced photodegradation of methylene blue dye and photoelectrochemical water splitting

Deepanshu Sharma, Mohd Faraz, Dheeraj Kumar, Devender Takhar, Balaji Birajdar, Neeraj Khare

Summary: In this study, V2O5/rGO nanocomposite was synthesized using a simple hydrothermal method, and its potential for photocatalysis and photoelectrochemical water splitting was validated. Compared to bare V2O5, V2O5/rGO nanocomposite exhibited improved photocatalytic activity and enhanced photoelectrochemical performance.

INORGANIC CHEMISTRY COMMUNICATIONS (2022)

Article Physics, Applied

Magnetically tunable enhanced performance of CoFe2O4-PVDF nanocomposite film-based piezoelectric nanogenerator

Arun Mondal, Mohd Faraz, Neeraj Khare

Summary: In this study, a flexible piezoelectric nanogenerator with tunable output power using a PVDF-CFO nanocomposite film was prepared. By incorporating ferromagnetic CFO nanoparticles into the PVDF matrix, the ferroelectric beta phase fraction of PVDF was enhanced, providing magnetic tunability to the PENG device. The output power of the PENG increased with the application of an external magnetic field, attributed to the decrease in resistance of the nanocomposite film.

APPLIED PHYSICS LETTERS (2022)

Article Physics, Multidisciplinary

Investigation of analog resistive switching in ZrO2 nanostructured film

Aman Sharma, Mohd Faraz, Neeraj Khare

Summary: In this article, the synthesis of ZrO2 nanostructure and the fabrication of resistive switching device [FTO/ZrO2/Ag] using the hydrothermal and spray coating methods are reported. The results show that the fabricated device exhibits gradual set and reset at 0.8V and -0.7V, respectively, confirming the presence of analog switching. The conduction mechanism in high resistive state and low resistive state has been explored using an I-V fitting model.

EUROPEAN PHYSICAL JOURNAL PLUS (2022)

Article Chemistry, Physical

Enhanced photoelectrochemical water splitting and photocatalytic degradation performance of visible light active ZnIn2S4/PANI nanocomposite

Mohit Khosya, Dheeraj Kumar, Mohd Faraz, Neeraj Khare

Summary: In this study, zinc indium sulfide coupled polyaniline (ZnIn2S4/PANI) nanocomposites were synthesized using a chemisorption method. The nanocomposites exhibited significant enhancement in photo-catalytic and photoelectrochemical (PEC) water splitting activity under visible light illumination. The improvement in photodegradation and PEC water splitting activity is attributed to extended visible light absorption, synergistic effect of PANI, and the formation of a type-II heterojunction, which facilitates charge separation and faster transfer efficiency of photogenerated charge carriers.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY (2023)

Article Physics, Multidisciplinary

Thermoelectric performance of (±) Camphor-10-sulfonic acid doped polyaniline/graphitic carbon nitride composite films

Manoj Singh, Amish Kumar Gautam, Mohd Faraz, Neeraj Khare

Summary: Flexible composite films of PANI-CSA doped with g-C3N4 were synthesized and their thermoelectric properties were studied. The results showed that at 363K, the power factor of the sample doped with 20wt.% g-C3N4 was about 3 times higher than the pristine sample, which was attributed to the enhanced carrier energy filtering effect at the interfaces of PANI-CSA and g-C3N4.

EUROPEAN PHYSICAL JOURNAL PLUS (2022)

Article Engineering, Chemical

Visible light active ZnIn2S4/g-C3N4 heterostructure nanocomposite photoelectrode for efficient photoelectrochemical water splitting activity

Mohit Khosya, Dheeraj Kumar, Mohd Faraz, Neeraj Khare

Summary: Hexagonal zinc indium sulfide coupled g-C3N4 (H-ZnIn2S4/g-C3N4) nanocomposites were synthesized via chemisorption method, and their photoelectrochemical water splitting activity was investigated. The H-ZnIn2S4/g-C3N4 nanocomposites exhibited a 1.9 times higher photoelectrochemical performance than H-ZnIn2S4. The improved performance can be attributed to the formation of a type-II heterojunction, which enhances the separation and transfer of photogenerated charge carriers.

ADVANCED POWDER TECHNOLOGY (2023)

Article Chemistry, Multidisciplinary

Enhanced photocatalytic reduction of hexavalent chromium by using piezo-photo active calcium bismuth oxide ferroelectric nanoflakes

Mohit Khosya, Mohd Faraz, Neeraj Khare

Summary: Photocatalysis using solar energy is a promising solution for environmental pollution. This study focuses on the synthesis of ferroelectric calcium bismuth oxide (CBO) nanoflakes and investigates the impact of piezo-photo effect on the photoreduction of hexavalent chromium (Cr(VI)) under visible light. The CBO nanoflakes showed significant improvement in photoreduction efficiency, and a possible mechanism was proposed.

NEW JOURNAL OF CHEMISTRY (2022)

Article Biochemistry & Molecular Biology

Effects of aging and diabetes on the deformation mechanisms and molecular structural characteristics of collagen fibrils under daily activity

Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang

Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Tea polyphenols protect against Flavobacterium columnare-induced gill injury via suppression of oxidative stress, inflammation, and apoptosis in grass carp

Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng

Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Neddylation-dependent LSD1 destabilization inhibits the stemness and chemoresistance of gastric cancer

Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng

Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Review Biochemistry & Molecular Biology

Cellular and physiological roles of sigma factors in Vibrio spp.: A comprehensive review

Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim

Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

An eco-friendly composite hydrogel based on covalently crosslinked cellulose/poly (glycerol citrate) for thallium (І) removal from aqueous solutions

Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati

Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Enzyme-triggered approach to reduce water bodies' contamination using peroxidase-immobilized ZnO/SnO2/alginate nanocomposite

Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal

Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Bacterial cellulose microfiber reinforced hollow chitosan beads decorated with cross-linked melamine plates for the removal of the Congo red

Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri

Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Necrotic activity of ExhC from Mammaliicoccus sciuri is mediated by specific amino acid residues

Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti

Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Silk-hydrogel functionalized with human decellularized Wharton's jelly extracellular matrix as a minimally invasive injectable hydrogel system for potential nucleus pulposus tissue replacement therapy

Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal

Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

A uniform-unsaturated crosslinking strategy to construct injectable alginate hydrogel

Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma

Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

α-Tocopherol-loaded multi-layer nanoemulsion using chitosan, and dextran sulfate: Cellular uptake, antioxidant activity, and in vitro bioaccessibility

Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee

Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Stabilization of oil-in-water high internal phase emulsions with octenyl succinic acid starch and beeswax oleogel

Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao

Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Review Biochemistry & Molecular Biology

A review for the correlation between optic atrophy 1-dependent mitochondrial fusion and cardiovascular disorders

Bi-Feng Yao, Xiu-Ju Luo, Jun Peng

Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Review Biochemistry & Molecular Biology

Nanomaterials for detection of biomolecules and delivering therapeutic agents in theragnosis: A review

Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park

Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)

Article Biochemistry & Molecular Biology

Preparation and characterization of hemicellulose films reinforced with amino polyhedral oligomeric silsesquioxane for biodegradable packaging

Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan

Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2024)