Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway

Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-alpha in serum. mu assay showed that BCAP-1 had no cytotoxicity against 5-180 tumor cells. BCAP-1 enhanced the secretion of TNF-alpha and NO, and the transcripts of TNF-alpha. and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of I kappa B in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-kappa B signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features. (C) 2016 Elsevier B.V. All rights reserved.