Purification, structural characterization and antiproliferative properties of chondroitin sulfate/dermatan sulfate from tunisian fish skins

Chondroitin sulfate/dermatan sulfate GAGs were extracted and purified from the skins of grey triggerfish (GTSG) and smooth hound (SHSG). The disaccharide composition produced by chondroitinase ABC treatment showed the presence of nonsulfated disaccharide, monosulfated disaccharides Delta Di6S and Delta D14S, and disulfated disaccharides in different percentages. In particular, the nonsulfated disaccharide Delta DI0S of GTSG and SHSG were 3.5% and 5.5%, respectively, while monosulfated disaccharides Delta Di6Sand Delta Di4S were evaluated to be 18.2%, 59% and 14.6%, 47.0%, respectively. Capillary elecrophoresis analysis of GTSG and SHSG contained 99.2% and 95.4% of chondroitin sulfate/dermatan sulfate, respectively. PAGE analysis showed a GTSG and SHSG having molecular masses with average values of 41.72 KDa and 23.8 KDa, respectively. HCT116 cell proliferation was inhibited (p <0.05) by 70.6% and 72.65% at 200 mu g/mL of GTSG and SHSG respectively. Both GTSG and SHSG demonstrated promising antiproliferative potential, which may be used as a novel, effective agent. (C) 2016 Elsevier B.V. All rights