Hypoxia-induced activation of specific members of the NF-kB family and its relevance to pulmonary vascular remodeling

Background and objective: Pulmonary Hypertension (pH) is a chronic progressive disease. Endothelial cells (EC) play a central and critical role in the initiation and progression of pH. The NF-kappa B family (NF-kappa B1 (p50/p105), NF-kappa B2 (p52/p100), RelA (p65), RelB, and C-Rel) regulates a wide array of genes involved in inflammatory responses, cell proliferation, and survival. The involvement of specific NF-kappa B family members in the pathogenesis of hypoxia-induced pH remains to be determined. The objective of this study was to assess the specific role of individual NF-kappa B family members in mediating endothelial cell responses to hypoxia and its downstream effect on smooth muscle cell proliferation. Methods and results: NF-kappa B family members' expression were selectively reduced by siRNA in human pulmonary microvascular endothelial cells. Cells were then exposed to hypoxia (1%) for 24 h. Endothelin1, ICAM1 gene expression and Stat1 and Stat3 phosphorylation were assessed. Smooth muscle cells (SMC) proliferation was assessed by culturing them with EC conditioned media. Reduction of either NF-kappa B2 or RelA in EC, led to a significant decrease in Endothelinl and ICAM1 gene expression. C-Rel knockdown resulted in a significant increase in phosphorylated STAT1; both C-Rel and RelA knockdown significantly decreased phosphorylated STAT3 in EC. There was a significant reduction in SMC proliferation, and AKT/ERK phosphorylation in SMC, when cultured in ReIA knockdown, EC conditioned media. Conclusion: RelA in EC plays crucial role in hypoxia induced vascular remodeling and development of pH. Targeting ReIA in EC alleviates SMC proliferation as well as inflammation related processes.