Article
Hematology
Litao Guo, Yu Liu, HaiRong Zheng, Qindong Shi, Gang Wang
Summary: This study analyzed the anticoagulation effect of different local infusion methods of citrate in critically ill patients undergoing CRRT. The modified group had longer treatment time, lower transmembrane pressure, fewer citrate bag replacements, and more stable ionized calcium levels compared to the conventional group. The incidences of citrate accumulation and tubing coagulation were marginally lower in the modified group.
THERAPEUTIC APHERESIS AND DIALYSIS
(2023)
Review
Pediatrics
Carlos Carmona, Marianne E. E. Nellis, Oliver Karam
Summary: The purpose of this review is to discuss the hemostatic changes induced by ECMO, the balance between bleeding and clotting risks, and the risks associated with anticoagulation and hemostatic transfusions. Recent findings suggest that anti-Xa-guided strategies may decrease bleeding complications without increasing clotting events. However, further research is needed to confirm these results in randomized controlled trials.
CURRENT OPINION IN PEDIATRICS
(2023)
Article
Urology & Nephrology
Harmony H. M. To, Arthur M. C. Kwan, Natalie Y. Y. Leung, W. M. Chan, C. W. Ngai, Alfred S. K. Wong, Polly N. W. Tsai, Tammy S. K. Ma, Irene Yam, Pauline Yeung Ng, Desmond Y. H. Yap
Summary: This prospective study examined the diagnostic and predictive value of serum citrate levels for citrate accumulation (CA) in patients receiving regional citrate anticoagulation (RCA) continuous renal replacement therapy (CRRT). The study found that serum citrate levels showed good performance in diagnosing and predicting the development of CA.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Pediatrics
Yuelin Sun, Dong Li, Ke Bai, Feng Xu, Chengjun Liu, Hongxing Dang
Summary: This study compared two blood product transfusion protocols in children undergoing continuous renal replacement therapy (CRRT) to prevent clotting and citrate accumulation. The partial replacement of citrate transfusion protocol (PRCTP) was found to be superior to the direct transfusion protocol (DTP) as it did not increase the risks of citrate accumulation and hypocalcemia.
FRONTIERS IN PEDIATRICS
(2023)
Article
Materials Science, Biomaterials
Meili Zhang, Chris H. H. Chan, Jo P. Pauls, Clayton Semenzin, Carmen Ainola, Hui Peng, Changkui Fu, Andrew K. Whittaker, Silver Heinsar, John F. Fraser
Summary: This study investigated the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO. The hydrogels were able to achieve sustained release of heparin by adjusting the crosslinker content and water content. The coatings showed reduced platelet adhesion and prolonged plasma recalcification time, indicating good haemocompatibility.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Engineering, Biomedical
Karin Strobl, Stephan Harm, Ute Fichtinger, Claudia Schildbock, Jens Hartmann
Summary: The study demonstrates that anion exchange resins can adsorb citrate, leading to an increase in extracorporeal Ca(2+) concentration and an increased risk of clotting. This highlights the importance of considering increasing citrate infusion during the first hour of treatment to compensate for citrate adsorption.
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
(2021)
Article
Pediatrics
Haixia Huang, Xing Deng, Ke Bai, Chengjun Liu, Feng Xu, Hongxing Dang
Summary: Retrospective observational study showed that the use of regional citrate anticoagulant (RCA) in continuous renal replacement therapy (CRRT) is safe and effective for neonates. Adjusting the treatment parameters appropriately can minimize the incidence of citrate accumulation, which is not significantly correlated with age or corrected gestational age in neonates.
FRONTIERS IN PEDIATRICS
(2023)
Article
Pediatrics
Jun Hu, Chunxiao Wang, Ke Bai, Chengjun Liu
Summary: This study retrospectively analyzed the clinical application of RCA-mTPE in children with liver failure. The results showed that with proper protocol adjustment, RCA-mTPE can be used safely and effectively in these patients.
FRONTIERS IN PEDIATRICS
(2023)
Article
Immunology
Benjamin S. Storm, Judith K. Ludviksen, Dorte Christiansen, Hilde Fure, Kristin Pettersen, Anne Landsem, Bent Aksel Nilsen, Knut Dybwik, Tonje Braaten, Erik W. Nielsen, Tom E. Mollnes
Summary: This study aimed to investigate the effects of air embolism on thromboinflammation in pigs. The results showed that air embolism caused an increase in white blood cell count, activation of C3, and alterations in hemostatic parameters. Additionally, it triggered the release of cytokines in lung tissue. The study suggests that C3 inhibition may represent a therapeutic approach to attenuate the response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Livia Whiting, Nathan Bianchi, Mohamed Faouzi, Antoine Schneider
Summary: This study aimed to evaluate the dynamics and factors associated with decreased membrane performance in continuous kidney replacement therapy (CKRT). Mixed-effects logistic regression model analyses showed that time significantly affected these values and identified independent predictors of decreased membrane performance. Additionally, COVID-19 infection was not associated with an increased risk of decreased membrane performance.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoyan Tang, Dezheng Chen, Ling Zhang, Ping Fu, Yanxia Chen, Zhou Xiao, Xiangcheng Xiao, Weisheng Peng, Li Cheng, Yanmin Zhang, Hongbo Li, Kehui Li, Bizhen Gou, Xin Wu, Qian Yu, Lijun Jian, Zaizhi Zhu, Yu Wen, Cheng Liu, Hen Xue, Hongyu Zhang, Xin He, Bin Yan, Liping Zhong, Bin Huang, Mingying Mao
Summary: The two-stage regional citrate anticoagulation combined with sequential anticoagulation and standard calcium-containing dialysate can effectively reduce clotting events during hemodialysis, prolong dialysis time, with no significant difference in urea clearance compared to saline flushing.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2022)
Article
Pediatrics
Yasemin Ezgi Kostekci, Tanil Kendirli, Emrah Gun, Hacer Ucmak, Ferhan Demirtas, Merve Havan, Engin Kose, Emel Okulu, Fatma Tuba Eminoglu, Omer Erdeve, Begum Atasay, Saadet Arsan
Summary: The use of continuous kidney replacement therapy (CKRT) has increased in neonates, but finding a suitable anticoagulation method is challenging. This study aimed to demonstrate the efficacy and safety of regional citrate anticoagulation (RCA) in neonates. Retrospective analysis of 11 neonates treated with RCA-CKRT showed that RCA is an effective and safe anticoagulation method for neonates.
EUROPEAN JOURNAL OF PEDIATRICS
(2023)
Article
Dermatology
Thilo Gambichler, Maria Chatzipantazi, Rene Stranzenbach, Alicia Feldkamp, Laura Susok
Summary: This study aimed to assess blood parameter alterations during extracorporeal photopheresis (ECP) treatment. The results showed a significant decrease in erythrocytes, hemoglobin, and leukocytes after one ECP procedure, which persisted over time. Pathological decline in hemoglobin was observed in a higher proportion of patients after 6 and 12 months of ECP. Red blood cell volume and hematocrit were also decreased, and lymphocyte count further declined after 9 and 12 months of ECP. Coagulation parameters did not change significantly during ECP treatment.
DERMATOLOGIC THERAPY
(2022)
Article
Critical Care Medicine
Nina Buchtele, Michael Schwameis, Peter Schellongowski, Peter Quehenberger, Paul Knoebl, Ludwig Traby, Monika Schmid, Christian Schoergenhofer, Harald Herkner, Bernd Jilma, Thomas Staudinger
Summary: A high prevalence of reduced coagulation factor XII activity was observed in adult patients on extracorporeal membrane oxygenation, with lower activity associated with fewer thromboembolic complications. Coagulation factor XII activity may confound activated partial thromboplastin time measurements and potentially serve as a target for anticoagulation in ECMO.
CRITICAL CARE MEDICINE
(2021)
Article
Cell Biology
Andreas Margraf, Chang Liu, Mira Kullmar, Melanie Meersch, Jan Rossaint, Alexander Zarbock
Summary: Acute kidney injury significantly affects leukocyte function during CVVH treatment, impairing selectin-mediated slow rolling and chemokine-induced arrest. Systemic heparin and local citrate anticoagulants have no differential effect on leukocyte recruitment steps.
Article
Hematology
Stephan Harm, Claudia Schildboeck, Jens Hartmann
BLOOD PURIFICATION
(2020)
Article
Chemistry, Analytical
Victor U. Weiss, Katja Balantic, Ernst Pittenauer, Carla Tripisciano, Gernot Friedbacher, Viktoria Weber, Martina Marchetti-Deschmann, Guenter Allmaier
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2020)
Article
Multidisciplinary Sciences
Andrej M. Kielbassa, Marlene R. Leimer, Jens Hartmann, Stephan Harm, Markus Pasztorek, Ina B. Ulrich
Article
Cell Biology
Carla Tripisciano, Rene Weiss, Sobha Karuthedom George, Michael B. Fischer, Viktoria Weber
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Engineering, Biomedical
Karin Strobl, Stephan Harm, Ute Fichtinger, Claudia Schildbock, Jens Hartmann
Summary: The study demonstrates that anion exchange resins can adsorb citrate, leading to an increase in extracorporeal Ca(2+) concentration and an increased risk of clotting. This highlights the importance of considering increasing citrate infusion during the first hour of treatment to compensate for citrate adsorption.
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
(2021)
Article
Multidisciplinary Sciences
Stephan Harm, Claudia Schildboeck, Karin Strobl, Jens Hartmann
Summary: This study investigated the challenges in detecting endotoxins in human blood, and identified factors that influence endotoxin recovery, such as the effects of heparin and citrate. Endotoxin activity decreases over time, with maximum neutralization reached after 4-6 hours. High LAL activity of endotoxins in plasma correlates with strong monocyte activation. Further research is needed to identify the specific endotoxin neutralizing plasma components.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Sobha Karuthedom George, Lucia Laukova, Rene Weiss, Vladislav Semak, Birgit Fendl, Victor U. Weiss, Stephanie Steinberger, Guenter Allmaier, Carla Tripisciano, Viktoria Weber
Summary: This study demonstrates the differences in counting and characterizing platelet-derived EVs using different instruments and techniques. Flow cytometry and nanoparticle tracking analysis can accurately quantify the number of EVs, and reveal potential discrepancies in counts between different instruments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Silke Huber, Miriam A. Knoll, Michael Berktold, Reinhard Wuerzner, Anita Brindlmayer, Viktoria Weber, Andreas E. Posch, Katharina Mrazek, Sarah Lepuschitz, Michael Ante, Stephan Beisken, Dorothea Orth-Hoeller, Johannes Weinberger
Summary: Whole genome sequencing is a useful tool for monitoring resistance mechanisms in bacteria. This retrospective study found that the major resistance mechanisms in linezolid-resistant Staphylococcus epidermidis (LRSE) were mutations in 23S rRNA and the presence of the cfr gene, with most LRSE isolates showing high resistance levels to linezolid. Linezolid usage was identified as the main factor contributing to the development of resistance, and monitoring of the cfr gene in LRSE and restriction of linezolid usage are potential key steps to reduce resistance and transmission.
Article
Biochemistry & Molecular Biology
Marie Ebeyer-Masotta, Tanja Eichhorn, Rene Weiss, Vladislav Semak, Lucia Laukova, Michael B. Fischer, Viktoria Weber
Summary: Inflammation and thrombosis are closely related in various diseases, including ischemic events, sepsis, and COVID-19. Activated neutrophils, platelets, and other factors drive immunothrombosis. Research has shown that the use of heparin-functionalized adsorbents can effectively remove central effectors of immunothrombosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Infectious Diseases
Matthias Pilecky, Stephan Harm, Christoph Bauer, Jennifer Zottl, Robert Emprechtinger, Tanja Eichhorn, Claudia Schildboeck, Michael Ecker, Martin Willheim, Viktoria Weber, Jens Hartmann
JOURNAL OF INFECTION
(2022)
Review
Chemistry, Analytical
Erich Kny, Ciril Reiner-Rozman, Jakub Dostalek, Achim-Walter Hassel, Christa Noehammer, Florian Pfaffeneder-Mantai, Sabine Szunerits, Viktoria Weber, Wolfgang Knoll, Christoph Kleber
Summary: Healthcare is undergoing major transformations, necessitating the use of new technologies to support personalized medicine and disease prevention. Biomarkers in bodily fluids such as blood, urine, sweat, or saliva can be used for early diagnosis, disease monitoring, and predicting response to treatment. The development of non-invasive analysis methods has been driven by the need for patients to use and apply diagnostic approaches themselves. This review focuses on novel sensing systems for detecting various endogenous biomarkers associated with human diseases in bodily fluids and exhaled breath.
Article
Biochemistry & Molecular Biology
Stephanie Steinberger, Sobha Karuthedom George, Lucia Laukova, Rene Weiss, Carla Tripisciano, Martina Marchetti-Deschmann, Viktoria Weber, Gunter Allmaier, Victor U. Weiss
Summary: Extracellular vesicles (EVs) have potential applications in the medical field, and their stability during isolation and purification is crucial. This study investigated EV stability and particle numbers using nES GEMMA and NTA techniques, and found that nES GEMMA can provide important information on EV stability.
Article
Engineering, Biomedical
Vladislav Semak, Tanja Eichhorn, Rene Weiss, Viktoria Weber
Summary: In this study, blood-compatible matrices were developed by surface modification with polyzwitterionic polysulfobetainic and polycarboxybetainic coatings. The coating enhanced the blood compatibility of hydrophobic adsorbents, while had little effect on hydrophilic adsorbents. The coating maintained the adsorption capacity and reduced the activation of blood cells.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Medicine, General & Internal
Tanja Eichhorn, Silke Huber, Rene Weiss, Marie Ebeyer-Masotta, Lucia Laukova, Robert Emprechtinger, Rosa Bellmann-Weiler, Ingo Lorenz, Judith Martini, Markus Pirklbauer, Dorothea Orth-Hoeller, Reinhard Wuerzner, Viktoria Weber
Summary: Immunothrombosis is a central pathomechanism in sepsis and COVID-19, characterized by excessive inflammatory response and overactivation of the coagulation system, leading to multiple organ failure and death.
