期刊
INTERNATIONAL JOURNAL FOR PARASITOLOGY
卷 47, 期 9, 页码 569-583出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2017.03.007
关键词
Cattle tick; Low-Cot enrichment; MicroRNAs; Tick DNA repeats; PacBio error correction; Complex genome
类别
资金
- U. S. Department of Agriculture, Agricultural Research Service (USDA-ARS) [6205-32000-024-00D, 6205-32000-026-00D, 6205-32000-031-00D, 3094-32000-036-00D]
- Organization for Economic Co-operation and Development's Co-operative Research Programme: Biological Resource Management for Sustainable Agriculture Systems, Washington, USA
- USDA-ARS
The genome of the cattle tick Rhipicephalus microplus, an ectoparasite with global distribution, is estimated to be 7.1 Gbp in length and consists of approximately 70% repetitive DNA. We report the draft assembly of a tick genome that utilized a hybrid sequencing and assembly approach to capture the repetitive fractions of the genome. Our hybrid approach produced an assembly consisting of 2.0 Gbp represented in 195,170 scaffolds with a N50 of 60,284 bp. The Rmi v2.0 assembly is 51.46% repetitive with a large fraction of unclassified repeats, short interspersed elements, long interspersed elements and long terminal repeats. We identified 38,827 putative R. microplus gene loci, of which 24,758 were protein coding genes (>= 100 amino acids). OrthoMCL comparative analysis against 11 selected species including insects and vertebrates identified 10,835 and 3,423 protein coding gene loci that are unique to R. micro plus or common to both R. microplus and Ixodes scapularis ticks, respectively. We identified 191 microRNA loci, of which 168 have similarity to known miRNAs and 23 represent novel miRNA families. We identified the genomic loci of several highly divergent R. microplus esterases with sequence similarity to acetyl cholinesterase. Additionally we report the finding of a novel cytochrome P450 CYP41 homolog that shows similar protein folding structures to known CYP41 proteins known to be involved in acaricide resistance. (C) 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据