期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 44, 期 -, 页码 87-96出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2017.01.007
关键词
Procyanidins; Gallate group; T cells; IFN-gamma; T-bet
资金
- BRAIN: Integration research for agriculture and interdisciplinary fields
Procyanidins are widely found in plants and have anti-inflammatory activities. Procyanidin B2 (PCB2) inhibits production of proinflammatory cytokines in macrophages. However, there has been no study that has attempted to determine the effects of PCB2 gallates on T cell functions. In the present study, murine splenocytes were isolated and treated with PCB2 or PCB2 gallates in the presence of an anti-CD3 monoclonal antibody (mAb). PCB2 gallates, but not PCB2, inhibited proliferation and T cell activation of splenocytes after stimulation with the anti-CD3 mAb. In addition, PCB2 gallates, particularly 3,3-di-O-gallate (3,3-di-OG), significantly inhibited production of interferon (IFN)-y, interleukin (IL)-12p40, and IL-17 in splenocytes, but did not suppress IL-10 production. Intracellular staining revealed that the expression of IFN-gamma and IL-17 in both CD4(+) and CD8(+) T cells was obviously decreased by PCB2 3,3-di-OG compared with PCB2, PCB2 3-OG, and PCB2 3-OG. Treatment of isolated CD4+ and CD8+ T cells with procyanidins in the presence of the anti-CD3 mAb led to significant inhibition of IFNy production by PCB2 3,3-di-OG in both cell types. Furthermore, PCB2 3,3-di-OG suppressed the expression of transcription factors T-bet and ROR gamma t that regulate IFN-gamma and IL-17, respectively. In conclusion, we revealed a new mechanism through which PCB2 gallates inhibit IFN-gamma and IL-17 production in T cells by down-regulation of T-bet and ROR gamma t expression. Our results suggest that PCB2 gallates have a potential role in Thl/Thl 7-mediated diseases such as inflammatory and autoimmune diseases. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据