期刊
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
卷 174, 期 3-4, 页码 121-132出版社
KARGER
DOI: 10.1159/000481984
关键词
Fructo-oligosaccharides; Food allergy; Antigen-specific CD4(+) T cells; Mesenteric lymph nodes; Peyer's patches; T helper cells
资金
- Japan Society for the Promotion of Science [24580200, 15K07443]
- Grants-in-Aid for Scientific Research [17K15277, 17K07801, 24580200, 15K07443] Funding Source: KAKEN
Background: Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4(+) T cell responses in food allergy, using OVA23-3 mice. Methods: OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation. Single-cell suspensions were prepared from intestinal and systemic lymphoid tissues for cellular analysis. Cytokine production was measured by ELISA. Activation markers and intracellular cytokines in CD4+ T cells were analyzed by flow cytometry. Activated CD4+ T cells were purified to examine cytokine production. Results: Dietary intake of FOS provided moderate protection from the intestinal inflammation induced by the OVA-containing diet. FOS significantly reduced food allergy-induced Th2 cytokine responses in intestinal tissues but not in systemic tissues. FOS decreased OVA diet-induced IFN-.gamma+IL-4(+) double-positive CD4(+) T cells and early-activated CD45RB(high)CD69(+)CD4(+)T cells in the mesenteric lymph nodes. Furthermore, we confirmed that these CD45RB(high)CD69(+)CD4(+)T cells are able to produce high levels of IFN-gamma and moderate level of IL-4, IL-10, and IL-13. Conclusions: Dietary intake of FOS during the development of food allergy attenuates the induction of intestinal Th2 cytokine responses by regulating early activation of naive CD4(+) T cells, which produce both Th1 and Th2 cytokines. Our results suggest FOS might be a potential food agent for the prevention of food allergy by modulating oral sensitization to food antigens. (c) 2017 S. Karger AG, Basel
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