期刊
INSECT SCIENCE
卷 26, 期 2, 页码 229-239出版社
WILEY
DOI: 10.1111/1744-7917.12542
关键词
Bactrocera dorsalis; BdE75; Bdo-Let-7; ecdysone signaling pathway
类别
资金
- earmarked fund for the China Agricultural Research System [CARS-26]
- International Atomic Energy Agency's Coordinated Research Project [D42016]
- National Natural Science Foundation of China [31572008]
- Fundamental Research Funds for the Central Universities [2014PY005, 2662015PY129]
MicroRNAs (miRNAs) regulate various biological processes during insect development; however, their role in larval-pupal development in oriental fruit fly, Bactrocera dorsalis (Hendel) remains unknown. In the current study, we address the biological function of a conserved miRNA, Bdo-Let-7 in the regulation of BdE75 gene, which belongs to the ecdysone signaling pathway and participates in the larval-pupal development in B. dorsalis. Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3 ' untranslated region of BdE75 gene and suppresses its expression. The Bdo-Let-7 and BdE75 are also co-expressed in the larval-pupal stages and in different tissues of B. dorsalis. In in vivo experiments, the injection of Bdo-Let-7 agomir and antagomir in third instar larvae down- and up-regulated the expression of BdE75, respectively. The 20-hydroxyecdysone (20E) injection assay shows that 20E up-regulated the expression of Bdo-Let-7 on the 5th day of the larvae. Moreover, abnormal pupation and eclosion were observed after larval Bdo-Let-7 antagomir injection. Based on these results, we show that Bdo-Let-7 regulates the ecdysone signaling pathway through the exact dose of BdE75 gene, and is indispensable for normal larval-pupal development in B. dorsalis.
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