期刊
INSECT SCIENCE
卷 25, 期 6, 页码 946-958出版社
WILEY
DOI: 10.1111/1744-7917.12487
关键词
evolutionary arms race; glucosinolate detoxification; glucosinolate sulfatase; phylogenetic analysis; transcriptome analysis
类别
资金
- National Natural Science Foundation of China [31320103922, 31230061, 31301677]
- National Key Project of Fundamental Scientific Research (973 Programs) in China [2011CB100404]
- Natural Science Foundation of Fujian Province, China [2014 J01086]
- Outstanding Youth Fellowships for WYH at FAFU [xjq201403]
- Jinshan Scholar Fellowships at FAFU
The diamondback moth, Plutella xylostella (L.), uses sulfatases (SULF) to counteract the glucosinolate-myrosinase defensive system that cruciferous plants have evolved to deter insect feeding. Sulfatase activity is regulated by post-translational modification of a cysteine residue by sulfatase modifying factor 1 (SUMF1). We identified 12 SULF genes (PxylSulfs) and two SUMF1 genes (PxylSumf1s) in the P. xylostella genome. Phylogenetic analysis of SULFs and SUMFs from P. xylostella, Bombyx mori, Manduca sexta, Heliconius melpomene, Danaus plexippus, Drosophila melanogaster, Tetranychus urticae and Homo sapiens showed that the SULFs were clustered into five groups, and the SUMFs could be divided into two groups. Profiling of the expression of PxylSulfs and PxylSumfs by RNA-seq and by quantitative real-time polymerase chain reaction showed that two glucosinolate sulfatase genes (GSS), PxylSulf2 and PxylSulf3, were primarily expressed in the midgut of 3rd- and 4th-instar larvae. Moreover, expression of sulfatases PxylSulf2, PxylSulf3 and PxylSulf4 were correlated with expression of the sulfatases modifying factor PxylSumf1a. The findings from this study provide new insights into the structure and expression of SUMF1 and PxylSulf genes that are considered to be key factors for the evolutionary success of P. xylostella as a specialist herbivore of cruciferous plants.
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