Article
Agronomy
Danielly A. M. Rios, Alexandre Specht, Vania F. Roque-Specht, Daniel R. Sosa-Gomez, Julia Fochezato, Juaci Malaquias, Gislene L. Goncalves, Gilson R. P. Moreira
Summary: Hybridization between Helicoverpa armigera and H. zea was investigated, with heterosis observed during immature development. However, reproductive incompatibilities were also found between parental species and subsequent hybrids. Interspecific gene flow and introgression events were confirmed, suggesting the need to monitor hybridization and resistance to pesticides. Management strategies should be implemented to prevent interspecific crosses and minimize the selection of resistant individuals.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Mengbo Guo, Xueting Ren, Yang Liu, Guirong Wang
Summary: This study provides insights into the olfactory perception of Helicoverpa armigera, identifying the role of HarmOR30 in tuning to indole, a key nitrogen-containing compound mediating tritrophic interactions. The findings suggest a potential function for HarmOR30 in foraging and mediating interactions with the environment.
Article
Biology
Xiao-Bin Fan, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Xin-Lin Gong, Chen-Zhu Wang
Summary: The results of this study indicate that odorant receptors (ORs) play an essential role in pheromone communication, oviposition selection, and larval chemotaxis of Helicoverpa armigera. Inhibiting or silencing the expression of the odorant receptor co-receptor (Orco) could disrupt mate searching and host-seeking behaviors of moth pests.
Article
Biochemistry & Molecular Biology
Hao Guo, Ping-Ping Guo, Ya-Lan Sun, Ling-Qiao Huang, Chen-Zhu Wang
Summary: The study shows that both HarmPBP1 and HarmPBP2 are involved in the detection of Z11-16:Ald in the male antennae of Helicoverpa armigera. HarmPBP1 and HarmPBP2 were found to be localized in cells surrounding the olfactory receptor neurons expressing HarmOR13. However, they have little effect on the selectivity and response kinetics of HarmOR13.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Entomology
Dong Ai, Chenxi Dong, Bin Yang, Caihong Yu, Guirong Wang
Summary: The study analyzed the expression patterns of gustatory receptors in Helicoverpa armigera, focusing on the highly expressed fructose receptor HarmGR9 in the foregut and abdomen. Using the CRISPR/Cas9 system, the function of HarmGR9 was identified, revealing that knockout of the gene shortened larval development and increased food consumption in both larvae and adults. This research sheds light on the tissue distribution and feeding regulation of sugar-sense-related receptors in H. armigera.
Article
Agriculture, Multidisciplinary
Jiaoxin Xie, Tinghui Liu, Chaoqun Yi, Xiaoxu Liu, Haoyu Tang, Yang Sun, Wangpeng Shi, Adel Khashaveh, Yongjun Zhang
Summary: The study identified 37 odorant receptors (ORs) in the antennae of ladybird Hippodamia variegata, with HvarOR25 being specifically enriched. HvarOR25 was found to have a narrow tuning to six chemical ligands, and it showed attractive responses to four of these ligands in H. variegata. Molecular docking results indicated a high binding affinity between HvarOR25 and all ligands.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Guangyan Wu, Qingya Li, Xiao Liu, Hongmei Li-Byarlay, Bingjun He
Summary: The study investigated the electrophysiological properties of voltage-gated sodium channels in sensitive and pyrethroid resistant cotton bollworm, Helicoverpa armigera. The results showed significant differences in the fundamental electrophysiological properties of sodium channels between resistant and sensitive strains, as well as different responses to pyrethroid modification. Both deltamethrin and tefluthrin had effects on the closed state of sensitive sodium channels, with deltamethrin having higher affinity to the open state.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Physiology
Yipeng Liu, Sai Zhang, Yang Liu, Guirong Wang
Summary: This study analyzed the molecular mechanism of P. xylostella exposed to plant volatiles. The odorant receptor PxylOR11 was found to be expressed in the antennae, with higher expression levels in female moths. Functional analyses showed that PxylOR11 responded to three aromatic compounds. Electroantennogram analyses and dual-choice bioassays confirmed the repellent effect of these compounds on P. xylostella. The results suggest that PxylOR11 could be a potential target for designing olfactory regulators to control P. xylostella.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joy Das, Rakesh Kumar, Vivek Shah, K. P. Raghavendra, Ashwani Kumar Sharma
Summary: This study presents the first report on the cloning, recombinant expression, and functional characterization of the NAGK gene from Helicoverpa armigera. The gene plays a crucial role in the growth and development of H. armigera and could be considered as a compelling gene of interest for formulating novel pest management strategies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Tatsuya Suzuki, Seiji Yamato
Summary: Oxazosulfyl is a novel insecticide that acts by blocking voltage-gated sodium channels in insects, resulting in paralysis. This discovery sheds light on the mode of action of oxazosulfyl as a potential pest control agent in rice fields.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Rene Y. Y. Lawong, Fabian May, Etang C. C. Etang, Philipp Vorrat, Jonas George, Julia Weder, Dagmar Kockler, Matthias Preller, Mike Althaus
Summary: The epithelial sodium channel (ENaC) is crucial for maintaining sodium homeostasis and blood pressure control. It is regulated by extracellular sodium ions through a mechanism called sodium self-inhibition (SSI). In this study, we evaluated the effectiveness of an automated two-electrode voltage-clamp system in detecting ENaC activity and SSI in Xenopus oocytes. Despite some limitations, the system was able to accurately detect the SSI characteristics of different ENaC orthologs and variants associated with hypertension.
Article
Biochemical Research Methods
Richard Agren, Hugo Zeberg
Summary: This study demonstrates that using an Ag-AgBr electrode with saturated KBr solution can reduce micropipette resistance, thereby decreasing the capacitive transient in two-electrode voltage clamp recordings. Additionally, it shows that the exchange of chloride against bromide ions does not seem to affect oocyte physiology and ion channel kinetics.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Agriculture, Multidisciplinary
Huang Tian-yu, Zhang Rui-bin, Yang Lu-lu, Cao Song, Frederic Francis, Wang Bing, Wang Gui-rong
Summary: The study reveals the important role of the conserved Or23 clade in host plant detection in most aphid species, and shows that pea aphids mainly rely on their odorant receptors to discriminate among different leguminous plant hosts.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Physiology
Francesca Vacca, Ana S. Gomes, Marco De Gennaro, Ivar Ronnestad, Elena Bossi, Tiziano Verri
Summary: The transport capability of charged dipeptides in teleosts is related to the position of the charged amino acid. In Atlantic salmon, the transport currents of PepT1a and PepT1b are similar, while in zebrafish, PepT1a is highly dependent on the position of Asp and PepT1b has low transport currents.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Qi Wang, Yong Xiao, Xing-Kui An, Shuang Shan, Adel Khashaveh, Shao-Hua Gu, Yun-Hui Zhang, Yong-Jun Zhang
Summary: Sex pheromones are believed to be important in sexual communication of most insects. In this study, a candidate sex pheromone receptor AlinOR33 in Adelphocoris lineolatus was functionally characterized, showing its essential role in sexual behavior. AlinOR33 was mainly expressed in sensilla trichoid on the antennae of A. lineolatus and specifically tuned to four sex pheromone components.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Entomology
Ke Yang, Chen-Zhu Wang
Summary: This article focuses on functional characterization of pheromone receptors (PRs) in heliothine species, summarizing recent progress in identification of receptors tuned to principal sex pheromone components. The efficiency and comparison of three frequently used heterologous expression systems are discussed, along with research strategies and future research topics on PRs in heliothine species.
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
(2021)
Article
Biochemistry & Molecular Biology
Hao Guo, Ping-Ping Guo, Ya-Lan Sun, Ling-Qiao Huang, Chen-Zhu Wang
Summary: The study shows that both HarmPBP1 and HarmPBP2 are involved in the detection of Z11-16:Ald in the male antennae of Helicoverpa armigera. HarmPBP1 and HarmPBP2 were found to be localized in cells surrounding the olfactory receptor neurons expressing HarmOR13. However, they have little effect on the selectivity and response kinetics of HarmOR13.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Entomology
Nan-Ji Jiang, Bao-Tong Mo, Hao Guo, Jun Yang, Rui Tang, Chen-Zhu Wang
Summary: The study identified the sex pheromone components of the fall armyworm using gas chromatography and other methods, and found that Z9-14:Ac and Z7-12:Ac are the principal sex pheromone components. This research provides important insights for the control and management of the fall armyworm.
Article
Biochemistry & Molecular Biology
Hao Guo, Ling-Qiao Huang, Xin-Lin Gong, Chen-Zhu Wang
Summary: Researchers compared the function of pheromone receptors (PRs) in Helicoverpa armigera and H. assulta by expressing their PR repertoires in Drosophila T1 neurons. All PRs of the two species, except for OR11, have been deorphanized using a Drosophila expression system and a large panel of pheromone compounds. This study provides a valuable reference for understanding the peripheral coding of pheromones.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Hao Guo, Xin-Lin Gong, Guo-Cheng Li, Bao-Tong Mo, Nan-Ji Jiang, Ling-Qiao Huang, Chen-Zhu Wang
Summary: In this study, six PRs were identified through transcriptome sequencing in the fall armyworm, showing varying responses to major and minor sex pheromone components. It was confirmed that S. frugiperda uses both SfruOR56 and SfruOR62 to detect the minor sex pheromone component Z7-12:Ac. Additionally, four sensilla types responsible for detecting pheromone compounds were characterized using single sensillum recording.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biology
Xiao-Bin Fan, Bao-Tong Mo, Guo-Cheng Li, Ling-Qiao Huang, Hao Guo, Xin-Lin Gong, Chen-Zhu Wang
Summary: The results of this study indicate that odorant receptors (ORs) play an essential role in pheromone communication, oviposition selection, and larval chemotaxis of Helicoverpa armigera. Inhibiting or silencing the expression of the odorant receptor co-receptor (Orco) could disrupt mate searching and host-seeking behaviors of moth pests.
Article
Biochemistry & Molecular Biology
Ping-Ping Guo, Guo-Cheng Li, Jun-Feng Dong, Xin-Lin Gong, Lingyu Wang, Ke Yang, Jun Yang, Ling-Qiao Huang, Chen-Zhu Wang
Summary: Closely related species Helicoverpa armigera and Helicoverpa assulta have differences in host plant ranges and sex pheromones. The study investigates the genetic basis for gene expression divergence in the antennae of the two species, and explores the connection between gene expression and phenotypic differences. The research findings provide insights into the rapid evolutionary changes in important olfactory traits in moths.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Yan Chen, Pei-Chao Wang, Shuai-Shuai Zhang, Jun Yang, Guo-Cheng Li, Ling-Qiao Huang, Chen-Zhu Wang
Summary: This study investigates the function of the gustatory receptor Gr180 in Helicoverpa armigera caterpillars. The results show that Gr180 responds to coumarin and is also involved in sensing sinigrin and strychnine. These findings enhance our understanding of the gustatory coding mechanisms of herbivorous insects to deterrents.
Article
Biochemistry & Molecular Biology
Min Liu, Xue-Ying Zhang, Chen-Zhu Wang, De-Hua Wang
Summary: This study investigated whether muscle could become an important site of NST when BAT function is conditionally minimized in Brandt's voles. The results showed that vole were able to maintain the same levels of NST and body temperature during cold acclimation even after surgical removal of interscapular BAT. This indicates that skeletal muscle-based thermogenesis may compensate for BAT impairment in small mammals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Hao Guo, Bao-Tong Mo, Guo-Cheng Li, Zi-Lin Li, Ling-Qiao Huang, Ya-Lan Sun, Jun-Feng Dong, Dean P. Smith, Chen-Zhu Wang
Summary: This study identifies the sex pheromone and elucidates the olfactory basis of sex pheromone communication in Campoletis chlorideae, a parasitoid wasp. It demonstrates the importance of specific receptors in male attraction and mating behavior, and also shows the synergistic effects of the sex pheromone and a female attractant in increasing the parasitic efficiency of the wasp.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Bing Wang, Xue-Ying Zhang, Shuai Yuan, He-Ping Fu, Chen-Zhu Wang, De-Hua Wang
Summary: The sensitivity to heat differs between Mongolian gerbils and mid-day gerbils, and this difference is associated with mutations in the TRPV1 gene. Through analyzing the mRNA expression and protein sequences of TRPV1 in different organs of these two gerbil species, we identified the mutations and confirmed the haplotype diversity of TRPV1. These findings contribute to the understanding of the evolutionary mechanisms of heat sensitivity in small mammals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Xi-Zhong Yan, Zhi-Yu Wang, Li Ma, Cheng-Fei Song, Jin-Yu Zhao, Chen-Zhu Wang, Chi Hao
Summary: This study investigated the repellent mechanism of Plutella xylostella towards volatiles from damaged Mentha spicata using electroantennogram (EAG) and behavioral responses. Four compounds, namely 2,4-dimethylheptane, 4-methyloctane, 2-methylnonane, and myrcene, were found to induce EAG responses in female antennae and significantly repel P. xylostella adults. These compounds may serve as behavioral regulators for this important pest.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Agronomy
Jian Shi, Lei He, Juan Du, Chen-Zhu Wang, Zhangwu Zhao
Summary: This study identified a gustatory receptor, Gr43a, in Ostrinia furnacalis larvae, which plays a role in discriminating between nutritious and non-nutritious substances. The Gr43a is negatively regulated by the neuropeptide F system and offers insight into feeding strategies for animals to meet nutritional needs and gratification.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Xi-Zhong Yan, Li Ma, Xiao-Fei Li, Le Chang, Qing-Zhao Liu, Cheng-Fei Song, Jin-Yu Zhao, Xing-Tao Qie, Cai-Ping Deng, Chen-Zhu Wang, Chi Hao
Summary: This study identified green leaf volatiles and isothiocyanates as key olfactory cues for host selection of the diamondback moth. Trans-2-hexen-1-ol and isopropyl isothiocyanate were identified as attractive compounds that can be used in the biological control of the pest.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Bao-Tong Mo, Hao Guo, Guo-Cheng Li, Lin-Lin Cao, Xin-Lin Gong, Ling-Qiao Huang, Chen-Zhu Wang
Summary: This study investigates the olfactory coding mechanisms of host plant selection by comparing two closely related moth species, Helicoverpa assulta and Helicoverpa armigera. The results demonstrate species-specific response to certain compounds and identify potent oviposition attractants for managing pest populations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
