Article
Chemistry, Applied
Yujun Wan, Zhenxia Hu, Qinghua Liu, Liang Wang, Mitchell A. Sullivan, Robert G. Gilbert
Summary: This study compared the molecular structure of liver glycogen in both liver fibrosis and healthy rats, revealing that liver fibrosis results in an increase in beta particles and a decrease in long chains in liver glycogen. The activity of glycogen branching enzyme and glycogen phosphorylase also decreased in the liver fibrosis group. Transcriptomics and proteomics analysis showed a deficiency in mitochondrial function in the liver fibrosis group, which may contribute to the changes in glycogen structure.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Ziyi Wang, Qinghua Liu, Liang Wang, Robert G. Gilbert, Mitchell A. Sullivan
Summary: Liver glycogen, a branched glucose polymer in animals, plays a role as a blood-sugar buffer. This study aims to optimize sucrose density gradient centrifugation for preserving glycogen's molecular structure by varying sucrose solution density and testing a boiling denaturation step. Lower sucrose concentrations and the boiling step were found beneficial in obtaining a more structurally representative and preserved glycogen sample.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Ruoyun Wu, Lina Zhou, Yan Chen, Xiaoqin Ding, Yan Liu, Bei Tong, Han Lv, Xiuhua Meng, Jing Li, Tunyu Jian, Jian Chen
Summary: Loquat leaf contains sesquiterpene glycosides that can prevent type 2 diabetes by relieving insulin resistance and inflammation, as well as reshaping the gut microbiota.
Article
Endocrinology & Metabolism
Iliana Lopez-Soldado, Joan J. Guinovart, Jordi Duran
Summary: Increased liver glycogen content can ameliorate the phenotype of obesity and diabetes, but has no effect on lipid metabolism.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Integrative & Complementary Medicine
You Wu, Boju Sun, Xiaoyuan Guo, Lili Wu, Yaomu Hu, Lingling Qin, Tao Yang, Mei Li, Tianyu Qin, Miao Jiang, Tonghua Liu
Summary: ZSP treatment significantly improved glucose metabolism and weight gain in db/db mice by enhancing the PI3K/AKT signaling pathway, increasing the expression of genes related to glycogen synthesis and glycolysis, and reducing gluconeogenesis.
Article
Biochemistry & Molecular Biology
Saadia Zahid, Alcir L. L. Dafre, Antonio Currais, Jingting Yu, David Schubert, Pamela Maher
Summary: CMS121 has shown potential as a therapeutic option for metabolic disorders by protecting db/db mice from metabolic defects, reducing liver inflammation, and improving kidney damage markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Jie Gao, Xue Gu, Manqian Zhang, Xingwang Zu, Fukui Shen, Xiaotao Hou, Erwei Hao, Gang Bai
Summary: Ferulic acid (FA) improves lipid metabolism in diabetes by targeting long-chain acyl-CoA synthase 1 (ACSL1) and regulating mitochondrial membrane distribution, resulting in decreased triglyceride and cholesterol synthesis.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Mehmet Direnc Mungan, Kai Blin, Nadine Ziemert
Summary: Due to the continuous evolution of drug resistant bacteria, new antibiotics are urgently needed. Genome mining algorithms have uncovered millions of uncharacterized BGCs, but compound identification and characterization remain challenges. Researchers prioritized promising BGCs using self-resistance based strategies to predict the biological activities of potentially novel antibiotics.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Nilofer Shaikh, R. K. Linthoi, K. Swamy, Muthukumarasamy Karthikeyan, Renu Vyas
Summary: This study uses molecular docking, virtual screening, and dynamics simulations to identify potential therapeutic agents for various cancer types. The researchers found that certain drugs showed potential against different cancer targets. They also built a library of novel molecules and utilized virtual screening tools to repurpose drugs and identify lead compounds.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Hui-Ju Tseng, Wen-Chu Chen, Tien-Fen Kuo, Greta Yang, Ching-Shan Feng, Hui-Ming Chen, Tzung-Yan Chen, Tsung-Han Lee, Wen-Chin Yang, Keng-Chang Tsai, Wei-Jan Huang
Summary: In this study, the Pdia4 inhibitor PS1 was found to improve beta-cell function and reverse diabetes through the reduction of ROS production. PS1 also decreased cell death and dysfunction in the pancreatic beta-islets by inhibiting the enzymatic activity of Pdia4 and mitigating the interaction between Pdia4 and Ndufs3 or p22. This research is of great significance for understanding the pathogenesis of diabetes and identifying potential therapeutic targets.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Iliana Lopez-Soldado, Joan J. Guinovart, Jordi Duran
Summary: Liver glycogen plays a crucial role in regulating endurance capacity in mice by maintaining blood glucose levels, leading to improved exercise performance.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Cell Biology
Zui Tan, Hongbao Sun, Taixiong Xue, Cailing Gan, Hongyao Liu, Yuting Xie, Yuqin Yao, Tinghong Ye
Summary: Liver fibrosis is a result of abnormal wound repair response caused by chronic liver injuries, potentially leading to severe diseases. The detailed mechanism of reversing liver fibrosis is still unclear, posing a challenge in the development of anti-fibrosis drugs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Li Wang, Michael A. Crackower, Hao Wu
Summary: Inflammasome proteins are important drug targets for many diseases, but their drug discovery has been difficult due to challenges in solving high-resolution structures. Recent advances in the structural biology of NLRP3 and NLRP1 offer hope for structure-based drug design.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Abhinav Kanwal, Navjot Kanwar, Sanjay Bharati, Prateek Srivastava, Shailendra P. Singh, Salomon Amar
Summary: There are significant shortcomings in the drugs currently available for treating type 2 diabetes mellitus, and the global diabetic crisis remains severe. The development of new drugs is still faced with various challenges, including issues with drug purity and toxicity, as well as the discovery of new targets. The recent discovery of a carcinogenic contaminant in a commonly used first-line drug highlights the difficulty of drug development.
Article
Biochemistry & Molecular Biology
Yu Zhang, Xiumei Wu, Mengyun Xu, Tong Yue, Ping Ling, Tingyu Fang, Sihui Luo, Suowen Xu, Jianping Weng
Summary: This study conducted hepatic and serum proteomic analysis in a diabetic mouse model to identify differentially expressed proteins (DEPs). The analysis revealed the involvement of these DEPs in various pathways and biological processes. Validation of mRNA expression of related genes further confirmed the results obtained from proteomic screening. These findings potentially provide sensitive non-invasive serum biomarkers for diabetes and NAFLD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Yujun Wan, Zhenxia Hu, Qinghua Liu, Liang Wang, Mitchell A. Sullivan, Robert G. Gilbert
Summary: This study compared the molecular structure of liver glycogen in both liver fibrosis and healthy rats, revealing that liver fibrosis results in an increase in beta particles and a decrease in long chains in liver glycogen. The activity of glycogen branching enzyme and glycogen phosphorylase also decreased in the liver fibrosis group. Transcriptomics and proteomics analysis showed a deficiency in mitochondrial function in the liver fibrosis group, which may contribute to the changes in glycogen structure.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Applied
Xiaoyan Tan, Changfeng Li, Yeming Bai, Robert G. Gilbert
Summary: This study investigates the effects of protein fractions with different digestion properties on starch digestibility in chickpea seed. The results show that the major insoluble proteins affecting starch hydrolysis are the glutelin-like and globulin-like fractions. The content of the globulin-like fraction is negatively correlated with starch digestibility.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Keyu Tao, Xin Liu, Wenwen Yu, Galex K. S. Neoh, Robert G. Gilbert
Summary: By studying the starch structure, it was found that chalky grains have different molecular structural characteristics compared to translucent grains. Chalky parts have a larger ratio of large to small branched molecules and more short amylopectin chains, but fewer longer chains, which affect the higher-level structure of starch.
Article
Chemistry, Applied
Shuaibo Shao, Enpeng Li, Shiyao Yu, Xueer Yi, Xiaowei Zhang, Chuantian Yang, Robert G. Gilbert, Cheng Li
Summary: This study investigates the relationship between starch chain-length distribution, starch digestibility, and texture of Chinese steamed bread (CSB) made from different wheat flour varieties. The results show that the starch digestibility and texture of CSBs are closely related to the starch chain structures.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Xiaoyan Tan, Shaobo Zhang, Alpeshkumar K. Malde, Xinle Tan, Robert G. Gilbert
Summary: This study investigates the effects of endogenous proteins on starch digestion kinetics in chickpeas. It identifies potential proteinaceous alpha-amylase inhibitors belonging to albumin, globulin, and glutelin and explores their binding mechanisms with porcine pancreatic alpha-amylase (PPA) using docking simulation. The results show that small peptides (<3 kDa) from the hydrolysis of endogenous proteins exhibit strong inhibitory activity on PPA. It is found that the different types of alpha-AIs inhibit PPA activity by interacting with specific regions of the enzyme, hindering the formation of enzyme-starch complexes and reducing starch digestibility.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Changfeng Li, Yi Ji, Enpeng Li, Robert G. Gilbert
Summary: This study examined the starch structure and protein distribution that affect the stickiness of cooked rice. It found that extra-long amylose chains are leached out during cooking and can be entangled with proteins, influencing the stickiness of cooked rice.
FOOD HYDROCOLLOIDS
(2023)
Review
Nutrition & Dietetics
Yeming Bai, Robert G. Gilbert
Summary: Obesity and type II diabetes are closely related to rapid starch digestion. Pectins, as plant polysaccharides, have diverse effects on starch digestion and can influence it through multiple mechanisms simultaneously, such as increasing digesta viscosity, inhibiting amylase activity, and regulating in vivo physiological responses.
Review
Cell Biology
Chenping Du, Rani O. Whiddett, Irina Buckle, Chen Chen, Josephine M. Forbes, Amelia K. Fotheringham
Summary: Type 1 diabetes (T1D) is an autoimmune disease caused by a combination of genetic and environmental factors. The consumption of advanced glycation end products (AGEs) found in Western diets is believed to be a major environmental risk factor for T1D, as it interacts with the receptor for advanced glycation end products (RAGE). The progression of T1D varies in individuals, providing an opportunity for prevention strategies. Targeting the AGE-RAGE axis may be a potential therapeutic strategy for T1D prevention.
Review
Food Science & Technology
Cheng Li, Wenwen Yu, Robert G. Gilbert
Summary: Whole white rice is an important staple food, and its starch digestion rate and location in the gastrointestinal tract play a crucial role in human health. The structure of starch undergoes order-disorder transitions during cooking, which determines its digestibility. The length distributions of amylose and amylopectin chains influence the gelatinization properties of rice starch. The chain-length and molecular-size distributions of starch affect nucleation, crystal growth rates, and interactions during retrogradation. Various kinetics models have been developed to study starch digestibility, providing new insights into the structural basis of cooked whole rice. Different digestible fractions of starch with distinct digestion patterns have been discovered, and their kinetics are largely determined by fine molecular structures. This review summarizes current insights and future directions for understanding starch digestibility in whole cooked rice, with the aim of developing a healthier rice with slower starch digestibility.
Article
Food Science & Technology
Songnan Li, Duo Feng, Enpeng Li, Robert G. Gilbert
Summary: This study successfully incorporated zeaxanthin into corn starch granules as a carrier, improving its stability and bioavailability. The composites showed rapid release during in vitro intestinal digestion, indicating their potential use in living systems. These findings could be applied in designing effective starch-based carriers for enhanced storage stability and controlled release delivery.
Review
Food Science & Technology
Jihui Zhu, Yeming Bai, Robert G. Gilbert
Summary: Starch provides a significant portion of human food energy and its structural features impact human health. The chain length distribution (CLD) is a crucial structural feature that affects properties like starch digestibility. Digestion rate of starch-containing foods is closely linked to the prevalence and treatment of diseases such as diabetes, cardiovascular disease, and obesity. This review focuses on measuring CLDs, modeling the biosynthesis process, and understanding the relationship between CLD properties and health implications, with the potential to improve food properties through plant breeding.
Review
Food Science & Technology
Changfeng Li, Alex Wu, Robert G. Gilbert
Summary: This paper discusses the impact of extra-long chains (ELCs) on the functional properties and biosynthesis mechanism of starch. However, the evidence for the existence of ELCs is currently uncertain. The paper reviews the techniques for separating amylose/amylopectin and characterizing ELCs, and discusses the related studies. A model based on conventional biosynthesis enzymes is used to provide a rigorous definition of ELCs. The lack of a method to quantitatively separate all amylopectin without residual amylose hinders current investigations. Two-dimensional (2D) characterization can provide unambiguous evidence for the existence of ELCs, but the resolution of current methods needs improvement for more accurate conclusions.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2023)
Article
Chemistry, Applied
Jihui Zhu, Keyu Tao, Sangeeta Prakash, Changquan Zhang, Robert G. Gilbert, Qiaoquan Liu
Summary: The molecular structure of rice starch affects both its digestibility and palatability. By optimizing certain structural parameters, it is possible to achieve a balance between slower digestibility and acceptable palatability, providing guidance for rice breeding.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
Xueer Yi, Shuaibo Shao, Xiaowei Zhang, Wenwen Yu, Bin Zhang, Hongsheng Liu, Robert G. Gilbert, Cheng Li
Summary: By investigating the micromorphology, crystallinity polymorphisms, molecular interaction patterns, and in vitro starch digestibility of 3 rice varieties stored under 5 different temperature programs, it was found that storage temperature affects the inter- and intramolecular interactions of starch in cooked white rice and the digestion rate. Storage at 4°C was most effective in reducing starch digestibility. The digestion rate constant was determined by short-range amylopectin intermolecular interactions, while the digestion extent was determined by both inter- and intramolecular interactions among starch molecules as well as the long-range order of retrograded double helices.
Article
Biochemistry & Molecular Biology
Shaobo Zhang, Xiaolei Fan, Alpeshkumar K. Malde, Robert G. Gilbert
Summary: This study used molecular dynamics simulations and free energy calculations to predict the binding of different GBSSI mutants to amylose fragments and to predict the effects of mutations on enzyme activity. It was found that mutants with negative binding energy are more likely to have higher enzyme activity and amylose content. This research is helpful in the development of grains with improved functional properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)