Article
Pharmacology & Pharmacy
Hui Li, Qixiu You, Xiaoyan Feng, Conglong Zheng, Xianxiang Zeng, Hengyi Xu
Summary: Wound healing from bacterial infections is a challenge, especially with the emergence of antibiotic-resistant bacteria. This study explored the antibacterial effects of silver nanoparticles and Ag+ on Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. The results showed that both silver nanoparticles and Ag+ had excellent antibacterial effects in vitro, but in vivo, silver nanoparticles showed better antibacterial effects.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Qianhe Xu, Junmin Zhang
Summary: The thioredoxin system is crucial for cell redox homeostasis and regulating cell signaling pathways, making it an attractive target for cancer treatment. The development of anticancer drugs targeting this system is hindered by limited pharmacophore of inhibitors and slow progress in clinical research, requiring novel strategies to accelerate the system for cancer therapy, with synergistic targeting as a promising approach for chemotherapy resistance and sensitization immunotherapy.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Dongzhu Duan, Yanru Wang, Dabo Pan, Le Wang, Jian Xiao, Peng Song, Xiaojie Jin, Xiaoling Wang
Summary: DET targets TrxR and inhibits its activity, leading to cancer cell apoptosis through oxidative stress. Down-regulation of TrxR1 by shRNA increases cell sensitivity to DET. Targeting of TrxR1 by DET reveals a novel mechanism of action and enhances the potential of DET as a chemotherapeutic agent for cancers.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Editorial Material
Chemistry, Multidisciplinary
Lukas Zeisel
Summary: Lukas completed his undergraduate studies at LMU Munich, Germany, and conducted additional research internships at the University of California, Berkeley, and Bayer Pharmaceuticals. In his PhD research with Oliver Thorn-Seshold (LMU), he is focusing on developing chemical tools using rational reactivity-based design to visualize, modulate, and exploit cellular oxidoreductase activity in vivo. Besides his academic pursuits, Lukas is passionate about mountaineering, football, basketball, as well as vectorial graphics and Pokemon.
Article
Biology
E. Sandra Chocron, Kennedy Mdaki, Nisi Jiang, Jodie Cropper, Andrew M. Pickering
Summary: In this study, we found that overexpression of TrxR2 enhances metabolic function and improves resistance to metabolic dysfunction induced by high-fat diet in mice. This effect is achieved through enhanced mitochondrial function, partly through improvements in the tricarboxylic acid cycle and electron transport chain. These findings demonstrate the important role of TrxR2 and mitochondrial thioredoxin in metabolic regulation.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jing Liu, Youqiao Xu, Haojie Lu, Rong Wang, Zhuolu Xia, Changshun Zhao, Dechun Huang, Feng Jiang, Wei Chen
Summary: Three self-assembled nanoaggregates were constructed using different molecules, and their properties were characterized through various experiments. The results showed that nanoaggregates with disulfide groups exhibited better stability and improved cytotoxicity against cancer cells. Additionally, the nanoaggregates showed targeting ability to specific cellular components, leading to enhanced antitumor efficacy.
Article
Biotechnology & Applied Microbiology
Rong Huang, Guang-Qing Cai, Jian Li, Xi-Sheng Li, Hai-Ting Liu, Xue-Ling Shang, Jian-Dang Zhou, Xin-Min Nie, Rong Gui
Summary: A novel nano-drug delivery system, PLT@Ag-MOF-Vanc, was constructed for the treatment of MRSA infections, showing good antibacterial activity, biocompatibility, and targeting capabilities. It killed MRSA through multiple approaches and demonstrated superior anti-infective effects in a mouse MRSA pneumonia model compared to free vancomycin.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Immunology
Haoran Chen, Ning Yang, Liang Yu, Jiajia Li, Hui Zhang, Yahong Zheng, Mengran Xu, Yanyan Liu, Yi Yang, Jiabin Li
Summary: The combination of auranofin and phenethyl isothiocyanate (PEITC) has been found to enhance the antibacterial activity against Staphylococcus aureus. This combination treatment can prevent the emergence of auranofin resistance by increasing reactive oxygen species generation, disrupting bacterial cell structure, and inhibiting biofilm formation. In addition, this combination treatment effectively restores the susceptibility to auranofin and eliminates S. aureus pathogens, including methicillin-resistant S. aureus (MRSA).
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yijia Yang, Shibo Sun, Weiping Xu, Yue Zhang, Rui Yang, Kun Ma, Jie Zhang, Jianqiang Xu
Summary: This study investigated the inhibitory effect of piperlongumine on TXNRD1 and its molecular mechanism, and revealed the potential possibility of inhibiting TXNRD1 to enhance cancer cell death.
Article
Biochemistry & Molecular Biology
Junmin Zhang, Yaxiong Chen, Jianguo Fang
Summary: The inhibition of thioredoxin reductase (TrxR) is vital for the development of antineoplastic drugs and radiosensitizers. In this study, it is found that micheliolide (MCL), derived from Michelia, not only induces oxidative stress-mediated apoptosis in HeLa cells but also sensitizes ionizing radiation (IR) treatment by targeting TrxR. Mechanistic studies reveal that MCL covalently binds to TrxR at position 498 to inhibit its biological function. Knockdown of TrxR enhances the cytotoxicity and radiation response induced by MCL, supporting the potential of MCL as a prospective radiosensitizer and cancer chemotherapeutic agent.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Sicong Wang, Giovanna Di Trapani, Kathryn F. Tonissen
Summary: This review discusses the upregulation of Nrf-2 regulated antioxidant systems in lymphomas and their association with multiple signaling pathways involved in lymphoma development and progression. Targeting antioxidant systems, especially TrxR, may represent a new therapeutic approach for lymphoma.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Oncology
Dana M. Zaher, Wafaa S. Ramadan, Raafat El-Awady, Hany A. Omar, Fatema Hersi, Vunnam Srinivasulu, Ibrahim Y. Hachim, Farah I. Al-Marzooq, Cijo G. Vazhappilly, Salim Merali, Carmen Merali, Nelson C. Soares, Paul Schilf, Saleh M. Ibrahim, Taleb H. Al-Tel
Summary: SIMR1281 displayed a multi-target anticancer effect by dual inhibition of glutathione and thioredoxin reductases, inhibiting cell proliferation and inducing apoptosis by inducing DNA damage, perturbing the cell cycle, and pathway inactivation.
Review
Biochemistry & Molecular Biology
Denis Swolana, Robert D. Wojtyczka
Summary: The article introduces the effects of Staphylococcus epidermidis and Staphylococcus aureus on human health, as well as silver nanoparticles as a potential new substance for neutralizing bacterial cells. By reviewing the data on the activity of silver nanoparticles in inhibiting bacterial growth and biofilm formation, it provides insights for future research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Karina Mayumi Sakita, Franciele Abigail Vilugron Rodrigues-Vendramini, Terezinha Inez Estivalet Svidzinski, Maria Sueli Felipe, Bernard Maigret, Erika Seki Kioshima
Summary: The development of multi-targeting inhibitors aims to enhance the antifungal effect by inhibiting two different targets in a single chemical entity. This study describes the discovery and selection of dual-inhibitors targeting thioredoxin reductase and glutathione reductase proteins of Aspergillus fumigatus. In vitro testing showed that Octenidine (OCT) exhibited promising antifungal activity against multiple fungi. OCT is believed to disrupt the cell membrane and inhibit both thioredoxin reductase and glutathione reductase, leading to increased ROS and fungal cell death.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Microbiology
Xinran Ren, Xuerui Guo, Chang Liu, Shisong Jing, Tiedong Wang, Li Wang, Jiyu Guan, Wu Song, Yicheng Zhao, Yan Shi
Summary: Hispidulin effectively inhibits the expression of the virulence factor alpha-hemolysin in Staphylococcus aureus without affecting bacterial growth.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Chemistry, Analytical
Zhu Jun Zhong, Zhong-Ping Yao, Zi-Qi Shi, Yang-Dan Liu, Li-Fang Liu, Gui-Zhong Xin
Summary: Nitric oxide (NO) plays an important physiological role, and a quantitative mass spectrometry probe approach using Amlodipine (AML) has been developed to measure intracellular NO levels with high sensitivity and low matrix interference. The AML/NO reaction is rapid and selective, making it a promising tool for NO detection in cells. This method enables specific measurement of endogenous and exogenous NO, offering a new approach for intracellular NO analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Liwen Huang, Pui-Kin So, Yu Wai Chen, Yun-Chung Leung, Zhong-Ping Yao
Summary: The study revealed that enhancing the inhibition of beta-lactamase inhibitory protein to class A beta-lactamases could be achieved by altering the flexibility of the interdomain linker and the rigidity of the interfacial loop.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Yan Huang, Haidi Yin, Baiying Li, Qian Wu, Yang Liu, Kristina Poljak, Julija Maldutyte, Xiao Tang, Mo Wang, Zhixiao Wu, Elizabeth A. Miller, Liwen Jiang, Zhong-Ping Yao, Yusong Guo
Summary: The study utilized an in vitro assay and quantitative mass spectrometry analysis to reveal cargo proteins enriched in transport vesicles through specific sorting machinery. It identified novel factors regulating vesicular trafficking and specific cargo clients of cellular factors, demonstrating the robustness and power of this approach for uncovering molecular mechanisms in protein transport.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Cheuk Chi A. Ng, Wai Man Tam, Haidi Yin, Qian Wu, Pui-Kin So, Melody Yee-Man Wong, Francis C. M. Lau, Zhong-Ping Yao
Summary: The study demonstrates the use of peptide sequences for durable and high-density data storage. By optimizing analytical protocols and developing software, the researchers successfully stored and retrieved text and music files using 18-mer peptides. This innovative method could potentially revolutionize data storage and stimulate advancements in related fields.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Jian-Ying Wang, Ying-Hao Yin, Jia-Yi Zheng, Li-Fang Liu, Zhong-Ping Yao, Gui-Zhong Xin
Summary: A LASSO-based prediction method was developed to predict CCS values of lipids by screening and optimizing molecular descriptors, significantly improving the accuracy and precision with the use of standard CCS values. The method could reduce false-positive identifications in IM-MS-based lipidomics.
Review
Chemistry, Analytical
Bin Hu, Zhong-Ping Yao
Summary: This article reviews the technical aspects and important applications of wooden-tip ESI-MS in various fields. Wooden-tip ESI-MS has shown significant achievements in the past 10 years and has the potential for further development and application.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biophysics
Jiang Liu, Wei Lu, Lu Zhang, Jiao Yang, Zhong-Ping Yao, Yongcheng He, Yingchun Li
Summary: This paper presented a tip-like electrochemical sensor prototype that integrated with a commercial pipettor, allowing semi-automatic assay procedure for sampling, detection, and rinsing. It introduced a novel multi-component sensing strategy for detection of UA, urea, and pH in urine samples using one single working electrode. This development may open new opportunities for electrochemical technology in point-of-care testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Zhennan Zhao, Xuan Tao, Yanxuan Xie, Qi Lai, Wenkai Lin, Kai Lu, Jinhui Wang, Wei Xia, Zong-Wan Mao
Summary: This study reports an anti-HER2 affibody-ruthenium catalyst hybrid that selectively catalyzes the activation of gemcitabine prodrug on tumor cells, resulting in suppression of tumor growth and reduced side effects.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Yanxuan Xie, Jingxin Chen, Bo Wang, Ai-Yun Peng, Zong-Wan Mao, Wei Xia
Summary: Quorum sensing is a cell-to-cell communication process that controls bacterial collective behaviors. A phosphate ester derivative of chrysin has been identified as a potent quorum sensing inhibitor of the human pathogen Pseudomonas aeruginosa, with higher anti-virulence activity compared to the parent molecule.
Article
Chemistry, Analytical
Jishou Piao, Lu Liu, Long Cai, Hyok Chol Ri, Xiangzi Jin, Huaze Sun, Xiangfan Piao, Hai-bo Shang, Xuejun Jin, Qiaosheng Pu, Yong Cai, Zhongping Yao, Donatella Nardiello, Maurizio Quinto, Donghao Li
Summary: This paper introduces a highly accurate size-based microparticle separation technique, in which magnetic nanoparticles are manipulated by an external magnetic field to collide with microparticles for high-accuracy separation. The method has achieved good results in the analysis of cancer cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Zihan Yang, Li Zhou, Tongxu Si, Siyuan Chen, Chengxi Liu, Kelvin Kaki Ng, Zesheng Wang, Zhiji Chen, Chan Qiu, Guopan Liu, Qingliang Wang, Xiaoyu Zhou, Liang Zhang, Zhongping Yao, Song He, Mengsu Yang, Zhihang Zhou
Summary: In this study, LH1 was found to promote the confined migration and metastasis of cancer cells by stabilizing SEPT2 and facilitating actin polymerization. These findings provide new insights into the migration and metastasis mechanisms of tumor cells.
Review
Chemistry, Analytical
Tsz-Fung Wong, Pui-Kin So, Zhong-Ping Yao
Summary: COVID-19 has been affecting the world for more than two years. The reverse transcription polymerase chain reaction (RT-PCR) method, although widely used, has limitations in terms of time and accuracy. Mass spectrometry (MS) offers high sensitivity, specificity, and speed, providing qualitative and quantitative information that RT-PCR cannot achieve. Various mass spectrometric approaches have been developed for rapid detection of SARS-CoV-2, including LC-MS/MS and MALDI-MS methods, as well as efforts for on-site detection with portable devices. This review summarizes these approaches, discusses their advantages, disadvantages, and future development.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xuewei Lin, Hao Wu, Gefei Huang, Qian Wu, Zhong-Ping Yao
Summary: This study developed a simple, rapid, and high-throughput approach for the authentication of red wine by combining matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and direct analysis in real time mass spectrometry (DART-MS). The approach, coupled with orthogonal partial least squares discrimination analysis (OPLS-DA), successfully classified 535 wines from 8 countries with high accuracy rates. This study demonstrated the feasibility of combining multiple mass spectrometric techniques for wine analysis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Jishou Piao, Lu Liu, Long Cai, Hyok Chol Ri, Xiangzi Jin, Huaze Sun, Xiangfan Piao, Hai-bo Shang, Xuejun Jin, Qiaosheng Pu, Yong Cai, Zhongping Yao, Donatella Nardiello, Maurizio Quinto, Donghao Li
Summary: The development of new technologies for the separation, selection, and isolation of microparticles has become increasingly important in recent years. This paper proposes a highly accurate size-based microparticle separation technique called rotating magnetic chromatography. By utilizing magnetic nanoparticles in a microfluidic separation channel, microparticles are separated based on their dimensions with high accuracy and reproducibility. This breakthrough technique has unmatched sub-micrometer cell separation capabilities and can unlock new perspectives in scientific fields such as medical oncology.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Rongshuang Xu, Sze In Madeleine Ng, Wing Sze Chow, Yee Ka Wong, Yuchen Wang, Donger Lai, Zhongping Yao, Pui-Kin So, Jian Zhen Yu, Man Nin Chan
Summary: This study investigated the chemical transformation of an alpha-pinene-derived organosulfate through heterogeneous OH oxidation and found that the oxidation can yield more oxygenated organosulfates with minimal formation of fragmentation products. The results suggest that functionalization reactions are likely the dominant processes during the oxidation, leading to the formation of products with hydroxyl and carbonyl functional groups. Overall, these observations provide insights into the sources, transformation, and fate of atmospheric organosulfates.
ATMOSPHERIC CHEMISTRY AND PHYSICS
(2022)