Article
Biophysics
Eric A. Vitriol, Melissa A. Quintanilla, Joseph J. Tidei, Lee D. Troughton, Abigail Cody, Bruno A. Cisterna, Makenzie L. Jane, Patrick W. Oakes, Jordan R. Beach
Summary: The processive movement of nonmuscle myosin 2 (NM2) has been observed in CAD cells derived from the central nervous system, particularly in protrusions that terminate at the leading edge. The processive velocities of NM2 in vivo are consistent with in vitro measurements, and it moves against the retrograde flow of lamellipodia. NM2 can still exhibit anterograde movement even in the absence of actin dynamics.
BIOPHYSICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Peter Nietmann, Kevin Kaub, Andrejus Suchenko, Susanne Stenz, Claas Warnecke, Mohan K. Balasubramanian, Andreas Janshoff
Summary: This study investigates the implications of different actins expressed in epithelial cells for network mechanics and dynamics. The authors find that gamma-actin forms stiffer networks compared to beta-actin, attributed to selective interactions with Mg2+-ions. They also observe that beta-actin networks show more small contraction foci, while gamma-actin networks have fewer but larger foci, indicative of a stronger interaction with myosin motors.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Alexandre Bergeron, Andra Brezai, Rami Shukr, Louis Villeneuve, Bruce G. Allen, Wasay M. S. Qureshi, Kathryn E. Hentges, Angelino Calderone
Summary: The study found that the appearance of nestin in neonatal rat ventricular cardiomyocytes was associated with a migratory phenotype, and the cotreatment with p38 mitogen-activated protein kinase inhibitor promoted the synthesis of nestin. Additionally, the reorganization of nonmuscle myosin IIB and its physical interaction with nestin may act together to facilitate the migratory phenotype in cardiomyocytes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
You-jin Jung, Jun Shik Choi, Jung-yeon Ryu, Zhihao Zhang, Yong-beom Lim
Summary: By designing self-adjusting supramolecular building blocks, we have developed a nanotube-shaped mRNA delivery platform with high thermodynamic stability. The nanotube can coat the flexible mRNA chain through cooperative interactions, leading to high uptake efficiency and controllable gene expression behavior in mammalian cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Acoustics
Ge Han, Yuexin Li, Qian Liu, Qian Chen, Haotian Liu, Baohua Kong
Summary: This study investigates the effect of high-intensity ultrasound combined with nonenzymatic glycation on the solubility and structural characteristics of myofibrillar proteins. The results show that this combination method can effectively improve the water solubility of myofibrillar proteins by interfering with their assembly process. This could potentially lead to new applications in the food industry.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Cell Biology
Florian Rouaud, Wenmao Huang, Arielle Flinois, Kunalika Jain, Ekaterina Vasileva, Thomas Di Mattia, Marine Mauperin, David A. D. Parry, Vera Dugina, Christine Chaponnier, Isabelle Mean, Sylvie Montessuit, Annick Mutero-Maeda, Jie Yan, Sandra Citi
Summary: Rouaud et al. demonstrate that cingulin and paracingulin interact with specific isoforms of myosin-2 through coiled-coil rod-mediated interactions and tether them to cell junctions. This interaction plays a crucial role in regulating junctional architecture and membrane properties. These findings provide insights into the molecular mechanisms that govern the localization and function of nonmuscle myosins at cell junctions.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Environmental Sciences
Yaoxing Liu, Xiaoyu Wu, Liping Dai, Xiaoyun Wu, Jianguo Ding, Riyao Chen, Rui Ding, Jianxi Liu, Bart Van der Bruggen
Summary: In this study, a bipolar membrane electrodialysis system was used to recover Ni in the form of Ni(OH)2 from plating wastewater without adding chemical reagents. The stable structure of Ni-EDTA can be destroyed to obtain free Ni2+ and combine with OH to form Ni(OH)2. The system showed high removal and recovery efficiency for Ni-EDTA in both synthetic and real wastewater samples.
Article
Optics
Wenhui Yu, Xiang Li, Bing Wang, Yanping Li, Binglin Shen, Junle Qu, Liwei Liu
Summary: Polarization-sensitive second-harmonic-generation microscopy (pSHGM) allows for the determination of molecular orientation. A wide-field pSHGM measurement scheme has been proposed to measure the second-order nonlinear polarization tensor, eliminating the assumptions of sample symmetry. The ability to record second harmonic signals simultaneously enables higher imaging frame rates compared to traditional point scanning methods.
Article
Virology
Jian Chen, Jian Liu, Zhilu Chen, Daobin Feng, Cuisong Zhu, Jun Fan, Shuye Zhang, Xiaoyan Zhang, Jianqing Xu
Summary: This study reveals the crucial role of nonmuscle myosin IIA (MYH9) in regulating influenza A virus (IAV) infection and replication. MYH9 can interact with the viral nucleoprotein (NP) and interrupt the formation of viral ribonucleoprotein (vRNP) complexes, leading to a suppression of viral RNA transcription and replication. These findings shed new light on the complicated interactions between IAV and its host, making MYH9 a potential target for antiviral drug development.
Article
Multidisciplinary Sciences
Sabrya C. Carim, Gilles R. X. Hickson
Summary: Animal cell cytokinesis requires the activation of the GTPase RhoA, which assembles a contractile ring at the plasma membrane. This process involves the scaffold protein Anillin, which recruits septins to the contractile ring. The N-terminus of Anillin scaffolds actomyosin, while the C-terminus binds Rho1-GTP and the presence of the Anillin PH domain is necessary for the recruitment of septins. This study demonstrates that the closure of the contractile ring involves the coordination of actomyosin and anillo-septin networks.
Article
Psychology, Biological
Shahram Bahrami, Alexey Shadrin, Oleksandr Frei, Kevin S. O'Connell, Francesco Bettella, Florian Krull, Chun C. Fan, Jan Rossberg, Guy Hindley, Torill Ueland, Anders M. Dale, Srdjan Djurovic, Nils Eiel Steen, Olav B. Smeland, Ole A. Andreassen
Summary: The study found that despite the lack of significant genetic correlation between major depression and general intelligence, there is a large shared genomic structure, with 92 shared genetic loci identified between the two phenotypes, indicating a certain genetic connection between them.
NATURE HUMAN BEHAVIOUR
(2021)
Article
Cell Biology
Shiri Avivi Kela, Kriti Sethi, Pei Yi Tan, Danesha Suresh, Hui Ting Ong, Perla G. Castaneda, Mustafi R. Amin, Tal Laviv, Erin J. Cram, Jan Faix, Ronen Zaidel-Bar
Summary: This study identified a novel mechanotransduction pathway in the C. elegans spermatheca, which involves tension-dependent localization of a RhoGEF and a positive feedback loop for RHO-1-MyosinII activation. It provides insights into how epithelial tubes can sense and adjust their contractility in response to internal pressure.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Physiology
Andrielle E. Capote, Ashley Batra, Chad M. Warren, Shamim A. K. Chowdhury, Beata M. Wolska, R. John Solaro, Paola C. Rosas
Summary: Experiments in this study looked at the role of beta-arrestin-2 in preserving cardiac function during aging, finding that beta-arrestin-2 signaling may play a significant role in protecting cardiac function in aging.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Psychiatry
Sandeep Grover, Himani Adarsh
Summary: This study aimed to evaluate the prevalence of mixed specifier in patients with unipolar and bipolar depression in India. The results showed that about one-fifth of patients with both types of depression exhibited mixed features during the acute phase of the illness, with no significant difference in the prevalence between the two groups.
ASIAN JOURNAL OF PSYCHIATRY
(2023)
Article
Physics, Multidisciplinary
A. Kozan, M. Ertas
Summary: The magnetic properties of a kinetic Ising system on a sandwiched trilayer with square lattices are studied using mean-field theory based on Glauber-type stochastic dynamics. The system exhibits both first and second-order phase transitions, and possesses rich dynamic phase diagrams dependent on Hamiltonian parameters. The time-dependence behavior of order parameters and thermal behavior of dynamic sublattice magnetizations are investigated.
EUROPEAN PHYSICAL JOURNAL PLUS
(2023)
Article
Hematology
Kasturi Pal, Roberta Nowak, Neil Billington, Rong Liu, Arit Ghosh, James R. Sellers, Velia M. Fowler
Article
Biochemistry & Molecular Biology
Pei-Wen Chen, Neil Billington, Ben Y. Maron, Jeffrey A. Sload, Krishna Chinthalapudi, Sarah M. Heissler
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Medicine, Research & Experimental
Andrew M. Breglio, Lindsey A. May, Melanie Barzik, Nora C. Welsh, Shimon P. Francis, Tucker Q. Costain, Lizhen Wang, D. Eric Anderson, Ronald S. Petralia, Ya-Xian Wang, Thomas B. Friedman, Matthew J. A. Wood, Lisa L. Cunningham
JOURNAL OF CLINICAL INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Jamis McGrath, Chun-Yu Tung, Xiayi Liao, Inna A. Belyantseva, Pallabi Roy, Oisorjo Chakraborty, Jinan Li, Nicolas F. Berbari, Christian C. Faaborg-Andersen, Melanie Barzik, Jonathan E. Bird, Bo Zhao, Lata Balakrishnan, Thomas B. Friedman, Benjamin J. Perrin
Summary: Research indicates that actin turnover is increased at the tips of mechanotransducing stereocilia during bundle maturation. These data show that actin is remodeled, likely by a severing mechanism, in response to mechanotransduction.
Article
Biochemistry & Molecular Biology
Christopher J. Alexander, Melanie Barzik, Ikuko Fujiwara, Kirsten Remmert, Ya-Xian Wang, Ronald S. Petralia, Thomas B. Friedman, John A. Hammer
Summary: Myosin 18A alpha is a myosin 2-like protein that co-assembles with myosin 2 in the spines of cerebellar Purkinje neurons, playing a role in dendritic spine maturation. It interacts with beta-Pix, promoting assembly of actin and myosin filaments in spines. Knockdown of Myosin 18A results in spine maturation defects, which can be rescued by an RNAi-immune version of Myosin 18A.
Article
Multidisciplinary Sciences
Rong Liu, Neil Billington, Yi Yang, Charles Bond, Amy Hong, Verl Siththanandan, Yasuharu Takagi, James R. Sellers
Summary: The study identified a binding protein for Drosophila myosin-7a called M7BP, which assembles myosin-7a into a motile complex enabling cargo transport and actin cytoskeletal remodeling. This complex also assembles actin bundles and filopodia-like protrusions in living cells.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Derek C. Sung, Mohsin Ahmad, Connie B. Lerma Cervantes, Yingfan Zhang, Robert S. Adelstein, Xuefei Ma
Summary: Mutations in non-muscle myosin 2A (NM2A) result in a spectrum of anomalies known as MYH9-Related Disease (MYH9-RD), causing macrothrombocytopenia, glomerulosclerosis, deafness, and cataracts in humans. Male mice with homozygous E1841K mutation are infertile due to defects in Sertoli cells, while compound double heterozygous males exhibit similar testis abnormalities. NM2A and 2B paralogs have redundant roles in Sertoli cells, essential for testes development and fertility.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ananya Tripathi, Charles Bond, James R. Sellers, Neil Billington, Yasuharu Takagi
Summary: Myosin proteins bind and interact with filamentous actin and perform specific functions in cells. The mechanobiology of myosins can be studied through in vitro motility assays, while fluorescence microscopy-based assays can be used to determine parameters such as actin translocation velocities. These techniques are valuable tools for investigating the dynamics of myosins and their role in cellular processes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Genetics & Heredity
Irit Hochberg, Leigh A. M. Demain, Julie Richer, Kyle Thompson, Jill E. Urquhart, Alessandro Rea, Waheeda Pagarkar, Agusti Rodriguez-Palmero, Agatha Schluter, Edgard Verdura, Aurora Pujol, Pilar Quijada-Fraile, Albert Amberger, Andrea J. Deutschmann, Sandra Demetz, Meredith Gillespie, Inna A. Belyantseva, Hugh J. McMillan, Melanie Barzik, Glenda M. Beaman, Reeya Motha, Kah Ying Ng, James O'Sullivan, Simon G. Williams, Sanjeev S. Bhaskar, Isabella R. Lawrence, Emma M. Jenkinson, Jessica L. Zambonin, Zeev Blumenfeld, Sergey Yalonetsky, Stephanie Oerum, Walter Rossmanith, Wyatt W. Yue, Johannes Zschocke, Kevin J. Munro, Brendan J. Battersby, Thomas B. Friedman, Robert W. Taylor, Raymond T. O'Keefe, William G. Newman
Summary: Human mitochondrial RNase P processes mitochondrial precursor tRNAs and is composed of three protein subunits. Pathogenic variants in PRORP, one of the subunits, lead to multisystem diseases with symptoms like hearing loss, ovarian insufficiency, and developmental delay. The disease-associated variants result in decreased mitochondrial tRNA processing, indicating that pathogenic variants in all three subunits can cause mitochondrial dysfunction with distinct clinical presentations.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Multidisciplinary Sciences
Sarah M. Heissler, Amandeep S. Arora, Neil Billington, James R. Sellers, Krishna Chinthalapudi
Summary: Using single-particle cryo-electron microscopy, we determined the near-atomic resolution structure of smooth muscle myosin-2 in the autoinhibited state, revealing the molecular mechanisms behind its regulation. Our study also identified a previously unknown conformational state in myosin-2 and explained the autoinhibition of its enzyme function in 10S. This research provides insights into the formation, stabilization, and relief of 10S through phosphorylation of the regulatory light chain.
Article
Audiology & Speech-Language Pathology
Cathy Yea Won Sung, Melanie Barzik, Tucker Costain, Lizhen Wang, Lisa L. Cunningham
Summary: The mouse utricle model system is the most reliable in vitro preparation for studying mature mammalian hair cells. However, quantifying hair cells efficiently and accurately has been a challenge. This study introduces a semi-automated method that combines immune reactivity for the specific transcription factor Pou4f3 with labeling of activated caspase 3/7 to detect surviving and apoptotic hair cells, enabling faster and more accurate quantification.
Article
Biochemistry & Molecular Biology
Thomas E. Sladewski, Paul C. Campbell, Neil Billington, Alexandra D'Ordine, James L. Cole, Christopher L. de Graffenried
Summary: Many single-celled eukaryotes have complex cell morphologies defined by microtubules. The shape of Trypanosoma brucei is mediated by a parallel array of microtubules. The orphan kinesin KLIF plays a crucial role in cytokinesis by crosslinking microtubules and stabilizing microtubule plus ends, forming organized bundles.
Article
Cell Biology
Kevin S. Cannon, Jose M. Vargas-Muniz, Neil Billington, Ian Seim, Joanne Ekena, James R. Sellers, Amy. S. Gladfelter
Summary: In this study, the researchers investigated the impact of different combinations of septin proteins on biophysical properties and cell morphogenesis. They discovered that a gene duplication event gave rise to two similar but distinct septin proteins, Cdc11a and Cdc11b, which showed differences in cell polarity defects and filament length.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Melissa A. Quintanilla, John A. Hammer, Jordan R. Beach
Summary: Non-muscle myosin 2 (NM2) motors play a crucial role in various cellular processes and are involved in cell migration, cytokinesis, epithelial barrier function, and tissue morphogenesis. This article provides a comprehensive review of the biochemical properties and regulation mechanisms of NM2, as well as recent studies using advanced imaging technologies to investigate NM2 assembly. It aims to appeal to both cytoskeletal enthusiasts and researchers outside the cytoskeleton field who are interested in cellular processes requiring actomyosin force production.
JOURNAL OF CELL SCIENCE
(2023)
Article
Neurosciences
Forrest Weghorst, Yeva Mirzakhanyan, Kian Samimi, Mehron Dhillon, Melanie Barzik, Lisa L. Cunningham, Paul D. Gershon, Karina S. Cramer
FRONTIERS IN CELLULAR NEUROSCIENCE
(2020)