Article
Microbiology
Mengqing Zhou, Yuping Huang, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
Summary: This study found that under alkaline pH nutrient limitation, a subpopulation of Vibrio alginolyticus cells enters the VBNC state with pivotal virulence regulator ToxR playing a key role. ToxR directly controls the expression of multiple genes, including those involved in ROS resistance, to regulate the formation of the VBNC state, leading to decreased virulence and activity when ToxR is impaired.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Bing Liu, Jianxiang Fang, Huizhen Chen, Yuehong Sun, Shan Yang, Qian Gao, Ying Zhang, Chang Chen
Summary: This study characterized GcvB in V. alginolyticus and determined its regulon through transcriptome and quantitative proteome analysis. The results suggest that GcvB plays a critical role in amino acid metabolism and virulence regulation in V. alginolyticus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Karan Gautam Kaval, Suneeta Chimalapati, Sara D. Siegel, Nalleli Garcia, Jananee Jaishankar, Ankur B. Dalia, Kim Orth
Summary: It has been proposed that bacteria use a process called transertion to synthesize and insert membrane proteins. This study provides evidence that Vibrio parahaemolyticus uses transertion to assemble its type III secretion system and inject virulence factors into host cells.
NATURE COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Xing Xiao, Wanxin Li, Yanfang Pan, Junlin Wang, Zhiqing Wei, Shi Wang, Na Wang, Jichang Jian, Huanying Pang
Summary: Lysine acetylation plays a crucial role in the metabolism and pathogenicity of V. alginolyticus under bile salt stress. A total of 1315 acetylated peptides were identified, and bioinformatics analysis revealed their involvement in various cellular processes and virulence-related pathways. This study provides a comprehensive understanding of lysine acetylation in V. alginolyticus under bile salt stress.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Immunology
Fan Mao, Kunna Liu, Nai-Kei Wong, Xiangyu Zhang, Wenjie Yi, Zhiming Xiang, Shu Xiao, Ziniu Yu, Yang Zhang
Summary: The study demonstrates that hosts exhibit different immune responses when encountering Vibrio pathogens of varying virulence, including activation of different immune pathways. Highly virulent Vibrio strains induce apoptosis in oyster cells, while less virulent strains enhance phagocytosis and cell adhesion capabilities.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yanni Zhao, Ningning Zhou, Jiamin Ren, Wang Liu, Chuang Zhou, Xuefeng Chen, Jieyu Zhao, Juanjuan Cao, Jinfang Yang, Jie Han, Huan Liu
Summary: This study comprehensively investigates the regulatory roles of the new cell density-dependent sRNA Qrr4 in V. alginolyticus, revealing its significant effects on the physiology, virulence, and metabolism of the bacteria. The disturbance of multiple metabolic pathways, including phospholipid, nucleotide, carbohydrate, and amino acid metabolic pathways, provides novel insights into the potential mechanism by which mutation of qrr4 interferes with cellular energy homeostasis and modulates the characteristics of V. alginolyticus.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yu Ding, Xiuxian Song, Zhiming Yu
Summary: In this study, the response mechanism of Vibrio alginolyticus in bottom flocs treated with modified clay (MC) was investigated at the transcriptome level. It was found that MC altered the morphology of the bacteria, damaged the membrane function, activated the antioxidant system, and caused changes in material and energy metabolism. Additionally, MC suppressed the expression of virulence factors in V. alginolyticus. These findings provide theoretical support for the development of a new model of healthy aquaculture.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Xin Yi, Xiaojin Xu, Xin Qi, Yunong Chen, Zhiqin Zhu, Genhuang Xu, Huiyao Li, Emma-Katharine Kraco, Haoyang Shen, Mao Lin, Jiang Zheng, Yingxue Qin, Xinglong Jiang
Summary: V. alginolyticus, a common pathogen in aquaculture, can cause severe economic losses. This study investigates the virulence regulation of V. alginolyticus and identifies the potential role of pstS and pstB genes in bacterial adhesion, biofilm formation, and motility. Understanding the relationship between gene expression and bacterial virulence could provide new insights for disease prevention.
Article
Fisheries
Hongyan Cai, Ying Ma, Yingxue Qin, Lingmin Zhao, Qingpi Yan, Lixing Huang
Summary: In this study, it was found that Vvrr2 is involved in the environmental adaptation and biofilm formation of Vibrio alginolyticus, and functions through the regulation of genes such as fliG, lps, and cheV. Overexpression of Vvrr2 negatively affects bacterial adhesion, growth, biofilm formation, motility, drug resistance, and virulence.
Article
Genetics & Heredity
Xin Yi, XiaoJin Xu, YuNong Chen, Genhuang Xu, ZhiQin Zhu, Huiyao Li, HaoYang Shen, Mao Lin, Wenyu Zhao, Jiang Zheng, XingLong Jiang
Summary: Due to antibiotic abuse, bacterial resistance has become a global problem, posing severe threats to aquaculture. The inhibiting effect of Fructus schisandrae on the growth of Vibrio alginolyticus was studied using RNA sequencing technology (RNA-seq), revealing differentially expressed genes and providing new insights for studying the virulence mechanism and potential treatment options for drug-resistant diseases.
Article
Immunology
Fuyuan Zeng, Huanying Pang, Ying Chen, Hongwei Zheng, Wanxin Li, Srinivasan Ramanathan, Rowena Hoare, Sean J. Monaghan, Xiangmin Lin, Jichang Jian
Summary: Recent studies have shown that lysine succinylation plays a crucial role in the pathophysiological processes of Vibrio alginolyticus, particularly in central metabolic pathways and virulence factors. The extensive crosstalk between acetylation and succinylation indicates a complex regulatory network in this pathogen, providing potential targets for the development of attenuated vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Jingxiao Cai, Yuan Hao, Rongjing Xu, Yuanxing Zhang, Yue Ma, Yibei Zhang, Qiyao Wang
Summary: LuxR has distinct binding characteristics in response to temperature changes, and it can regulate and control the expression of virulence genes in V. alginolyticus.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Environmental Sciences
Wenxiao Su, Yiqin Deng, Shujun Zang, Si'ao Gao, Juan Feng
Summary: Leucine-responsive regulatory protein (Lrp) plays a negative regulatory role in Vibrio alginolyticus, reducing its virulence by decreasing swarming motility, biofilm formation, extracellular protease secretion activity, and tolerance to copper ions. The expression of Lrp is influenced by various physicochemical factors.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Ben Yang, Shangyu Zhai, Xin Li, Jing Tian, Qi Li, Hongwei Shan, Shikai Liu
Summary: This study identified Vibrio alginolyticus as a causative pathogen associated with mass summer mortality of Pacific oysters cultured in Shandong, China, highlighting the potential impact of high temperature on its growth and pathogenicity. Detection of important virulence genes further supported the strong pathogenicity of V. alginolyticus. These findings suggest the importance of disease surveillance and genetic breeding program to develop oyster strains with high disease resistance.
Article
Fisheries
Yilin Zhang, Huimin Tan, Shiping Yang, Yucong Huang, Shuanghu Cai, Jichang Jian, Jia Cai, Qiwei Qin
Summary: This study found that attenuated function of DctP in V. alginolyticus leads to significant decreases in swarming motility, biofilm formation, ECPase formation, cell adhesion, and colonization ability, and a 40-fold increase in LD50 for the Delta dctP strain compared to the WT strain. Transcriptome analysis demonstrated that the deletion mutation of dctP could regulate the expression levels of 22 genes related to colonization, adhesion, and pathogenicity.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Shuanghu Cai, Yilin Zhang, Fan Wu, Rimin Wu, Shiping Yang, Ya Li, Youhou Xu
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Fisheries
Yanyan Chen, Fenglei Wu, Huanying Pang, Jufen Tang, Shuanghu Cai, Jichang Jian
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Fisheries
Guixiang Lin, Fan Da, Xiaoju Wan, Yucong Huang, Shiping Yang, Jichang Jian, Shuanghu Cai
Summary: This study found that using APS and GLP as adjuvants for DNA vaccine could significantly enhance the immune response in pearl gentian grouper, including antibody titres and enzyme activities. These adjuvants also up-regulated the expression levels of multiple immune-related genes. After vaccination, the groups with APS and GLP had significantly higher survival rates when challenged with Vibrio harveyi.
JOURNAL OF FISH DISEASES
(2023)
