Article
Biochemistry & Molecular Biology
Minkyoung Sung, Soo-Eun Sung, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, KilSoo Kim, Ju-Hyeon Lim, Gun Woo Lee, Hyo-Deog Rim, Byung-Soo Kim, Seunghee Won, Kyungmin Kim, Seoyoung Jang, Min-Soo Seo, Jungmin Woo
Summary: This study analyzed miRNAs in serum-derived neuronal exosomes to investigate potential biomarkers of acute stress, revealing specific miRNAs in nEVs from an acute severe-stress animal model. These miRNAs show differential expression over time, with the most prominent brain damage observed 7 days after foot shock treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Thiery De Serres-Berard, Siham Ait Benichou, Dominic Jauvin, Mohamed Boutjdir, Jack Puymirat, Mohamed Chahine
Summary: DM1 is a dominant genetic disease that can be treated by altering the chemical design of antisense oligonucleotides. Various strategies involving chemical modifications and conjugation with specific functional molecules have been shown to improve the potency in muscle and cardiac tissues. In addition, intrathecal administration can be used to treat central nervous system defects in DM1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sarah U. Morton, Christopher R. Sefton, Huanqing Zhang, Manhong Dai, David L. Turner, Michael D. Uhler, Pankaj B. Agrawal
Summary: miRNAs play a key role in regulating mRNA during processes like muscle differentiation. Understanding miRNA targets can provide insights into muscle biology and gene expression changes caused by diseases. The study found that differentially expressed miRNAs are enriched for functions related to calcium signaling and sarcomere formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Al Asmaul Husna, Md Mahfuzur Rahman, Yu-Chang Lai, Hui-Wen Chen, Md Nazmul Hasan, Takayuki Nakagawa, Naoki Miura
Summary: This study identified several differentially expressed miRNAs in exosomes of canine melanoma cells, with certain miRNAs significantly increased in plasma exosomes of metastatic melanoma patients. These miRNAs could serve as potential biomarkers for identifying metastatic melanoma and improving prognosis, with sensitivity and specificity over 85% for distinguishing between non-metastatic and metastatic patients.
PIGMENT CELL & MELANOMA RESEARCH
(2021)
Article
Biology
Lauren L. Ozimski, Maria Sabater-Arcis, Ariadna Bargiela, Ruben Artero
Summary: Myotonic dystrophy type 1 (DM1) is the most prevalent form of muscular dystrophy in adults with no current treatment options. The disease is mainly characterised by muscle pathology and affects patients worldwide. Research into various pathways affected in DM1 may provide new therapeutic targets.
BIOLOGICAL REVIEWS
(2021)
Article
Medicine, General & Internal
Yingying Xie, Wenwen Chen, Mengqian Zhao, Yuhai Xu, Hao Yu, Jianhua Qin, Hongjing Li
Summary: Arthritis affects approximately 250 million people worldwide and early diagnosis is crucial for treatment. The study found that miRNA expression in synovial fluid differs from that in serum, which can contribute to the research and discovery of early diagnostic biomarkers for joint diseases.
Review
Biochemistry & Molecular Biology
Janel A. M. Peterson, Thomas A. A. Cooper
Summary: Myotonic dystrophy (DM) is a highly variable multisystemic disorder that affects one in 8000 individuals. Although most research has focused on the symptoms and pathological mechanisms in muscle and brain, surveys have found a high prevalence of gastrointestinal (GI) symptoms in DM patients. Clinical studies have identified chronic and progressive dysfunction in various parts of the GI system. The pathological mechanisms leading to this dysfunction are not well understood, but it is suggested that misregulation of alternative splicing in smooth muscle may contribute to GI dysfunction in DM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Laura Yedigaryan, Ester Martinez-Sarra, Giorgia Giacomazzi, Nefele Giarratana, Bernard K. van der Veer, Alessio Rotini, Silvia Querceto, Hanne Grosemans, Alvaro Cortes-Calabuig, Sara Salucci, Michela Battistelli, Elisabetta Falcieri, Rik Gijsbers, Mattia Quattrocelli, Kian Peng Koh, Liesbeth De Waele, Gunnar M. Buyse, Rita Derua, Maurilio Sampaolesi
Summary: This study identifies an extracellular vesicle-derived miRNA signature that enhances the myogenic potential of myogenic stem cells, leading to improvements in muscle degeneration and muscle wasting related diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Orthopedics
Zhibang Sun, Junjie Shi, Chenyang Yang, Xukun Chen, Jiaqi Chu, Jing Chen, Yuan Wang, Chenxin Zhu, Jinze Xu, Guozhen Tang, Song Shao
Summary: This study aims to identify PMOP-associated circulating exosomal miRNAs and evaluate their diagnostic performance. The results suggest that plasma exosomal miRNAs have the potential to serve as noninvasive diagnostic biomarkers for PMOP.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2023)
Review
Genetics & Heredity
Jinyu Xu, Weitie Wang, Yong Wang, Zhicheng Zhu, Dan Li, Tiance Wang, Kexiang Liu
Summary: This review discusses the role of exosomes and the biomolecules they contain in the prevention, diagnosis, and treatment of cardiovascular diseases. By examining the results of published studies, the review reveals the mechanisms of exosomal miRNAs in various cardiovascular diseases.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Vanessa Todorow, Stefan Hintze, Alastair R. W. Kerr, Andreas Hehr, Benedikt Schoser, Peter Meinke
Summary: Myotonic dystrophy type 1 (DM1) is a disease caused by CTG-repeat expansions that primarily affects muscles and brain. In addition to known information on alternative splicing of genes involved in the pathology, there may be other factors contributing to the disease development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Xiomara Fernandez-Garibay, Maria A. Ortega, Estefania Cerro-Herreros, Jordi Comelles, Elena Martinez, Ruben Artero, Juan M. Fernandez-Costa, Javier Ramon-Azcon
Summary: The article describes the development of the first in vitro 3D model of DM1 human skeletal muscle, which shows highly aligned myotubes from both healthy and DM1 cells. The 3D micropatterning significantly improved DM1 cell differentiation into multinucleated myotubes, suitable for drug testing. AntagomiR-23b treatment in bioengineered DM1 skeletal muscle microtissues demonstrates significant therapeutic effects in rescuing molecular DM1 hallmarks and structural phenotypes.
Review
Biochemistry & Molecular Biology
Jessica Stoodley, Francisco Vallejo-Bedia, David Seone-Miraz, Manuel Debasa-Mouce, Matthew J. A. Wood, Miguel A. Varela
Summary: DM1 is a common muscular dystrophy that can potentially be treated with antisense therapy. Delivery of antisense molecules to target tissues remains a challenge, but conjugation to lipids, peptides, or antibodies has shown promise in improving therapeutic efficacy and tissue penetration. Clinical trials of antisense therapies are currently underway, raising hopes for an approved treatment for DM1 patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yang Du, Rui Qiu, Lei Chen, Yuewen Chen, Zhifeng Zhong, Peng Li, Fangcheng Fan, Yong Cheng
Summary: This study investigated the potential molecular mechanism of remote ischemic preconditioning (RIPC)-mediated neuroprotection. By analyzing the differential metabolites and proteins in the serum exosomes of RIPC participants and control subjects, it was found that these differentially expressed substances were related to pathways associated with neurodegenerative diseases. The results suggest that serum exosomal metabolites can serve as promising biomarkers for RIPC and provide a rich dataset and framework for future analyses of cerebral ischemia-reperfusion injury under ischemia/reperfusion conditions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Clinical Neurology
Matteo Garibaldi, Tommaso Nicoletti, Elisabetta Bucci, Laura Fionda, Luca Leonardi, Stefania Morino, Laura Tufano, Girolamo Alfieri, Antonio Lauletta, Gioia Merlonghi, Alessia Perna, Salvatore Rossi, Enzo Ricci, Jorge Alonso Perez, Tommaso Tartaglione, Antonio Petrucci, Elena Maria Pennisi, Marco Salvetti, Gary Cutter, Jordi Diaz-Manera, Gabriella Silvestri, Giovanni Antonini
Summary: In a large cohort of DM1 patients, muscle involvement was characterized by fat replacement, muscle atrophy and STIR positivity, which correlated with disease severity and duration. The study also identified STIR hyperintensity as an early marker of muscle involvement, providing potential therapeutic targets for future clinical trials.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Health Policy & Services
Kevin T. Stroupe, Neil Jordan, Joshua Richman, Gary R. Bond, Terri K. Pogoda, Lishan Cao, Stefan G. Kertesz, Tassos C. Kyriakides, Lori L. Davis
Summary: This study compares the cost-effectiveness and return on investment of Individual Placement and Support (IPS) with transitional work (TW) programs in treating military veterans with PTSD. IPS is more costly but more effective, while TW is less expensive but less effective. Overall, IPS improves employment outcomes for PTSD patients, while TW is cheaper but less effective.
ADMINISTRATION AND POLICY IN MENTAL HEALTH AND MENTAL HEALTH SERVICES RESEARCH
(2022)
Article
Environmental Sciences
Sergio Martinez-Campos, Panayiota Pissaridou, Katerina Drakou, Christos Shammas, Kostas Andreou, Miguel Gonzalez-Pleiter, Francisca Fernandez-Pinas, Francisco Leganes, Roberto Rosal, Michalis I. Koutinas, Konstantinos Kapnisis, Marlen I. Vasquez
Summary: Plastics can act as vectors of microorganisms and transport bacteria, including pathogens, between different environments. This study found that the bacterial community adhered to greenhouse plastics evolves during the process from their use to entering the ocean.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Clinical Neurology
Andrie Koutsoulidou, Demetris Koutalianos, Kristia Georgiou, Andrea C. Kakouri, Anastasis Oulas, Marios Tomazou, Tassos C. Kyriakides, Andreas Roos, George K. Papadimas, Constantinos Papadopoulos, Evangelia Kararizou, George M. Spyrou, Eleni Zamba Papanicolaou, Hanns Lochmueller, Leonidas A. Phylactou
Summary: In this study, we identified novel serum-based miRNA biomarkers for rare subtypes of muscular dystrophy through high-throughput next-generation RNA sequencing. Our results suggest that these miRNAs may serve as potential biomarkers for rare muscular dystrophies.
NEUROMUSCULAR DISORDERS
(2022)
Article
Health Policy & Services
Neil Jordan, Kevin T. Stroupe, Joshua Richman, Terri K. Pogoda, Lishan Cao, Stefan Kertesz, Tassos C. Kyriakides, Gary R. Bond, Lori L. Davis
Summary: Among veterans with PTSD, supported employment using the IPS model has shown better employment and functional outcomes compared to usual vocational rehabilitation services. This study compared the health services use and associated costs between IPS and usual care (TW program) among unemployed veterans. The findings suggest that IPS participants had higher costs primarily due to increased utilization and costs of vocational services during the intervention period. However, these differences declined in the postintervention period.
PSYCHIATRIC SERVICES
(2022)
Article
Immunology
Min Zhao, Rebecca Slotkin, Amar H. Sheth, Lauren Pischel, Tassos C. Kyriakides, Brinda Emu, Cynthia McNamara, Qiaosu Shi, Jaden Delgobbo, Jin Xu, Elizabeth Marhoffer, Aleagia Mercer-Falkoff, Jurgen Holleck, David Ardito, Richard E. Sutton, Shaili Gupta
Summary: Multiple clinical factors affect the strength and duration of immune responses after COVID-19 vaccination. Malignancy reduces the immune response after the third dose, indicating the need for clinically guided vaccine dosing regimens.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Megan Lee, Danielle Cosentino, Tassos C. Kyriakides, Tricia Cavallaro, Gary Stack, Shaili Gupta
Summary: This study aimed to characterize the clinical and demographic factors affecting clinical outcomes of COVID-19 and describe viral epidemiology among unvaccinated Veterans in New England. The study found that in a largely male and older cohort, poor outcomes were correlated with increasing age. Most Veterans in New England were infected with B.1 sublineages with the D614G mutation in 2020 and early 2021, while the B.1.617.2 lineage predominated after July 2021.
JOURNAL OF HOSPITAL MEDICINE
(2022)
Article
Nutrition & Dietetics
Amal Kaddoumi, Thomas S. Denney, Gopikrishna Deshpande, Jennifer L. Robinson, Ronald J. Beyers, David T. Redden, Domenico Pratico, Tassos C. Kyriakides, Bonian Lu, Anna N. Kirby, Darren T. Beck, Nancy D. Merner
Summary: Extra-virgin olive oil (EVOO) and refined olive oil (ROO) have beneficial effects on individuals with mild cognitive impairment (MCI), with EVOO improving clinical dementia rating and behavioral scores, reducing blood-brain barrier permeability, enhancing brain connectivity, and reducing blood amyloid-beta levels. ROO improves clinical dementia rating and functional brain activation.
Article
Agronomy
Konstantinos Kapnisis, Antonios Chrysargyris, Marianna Prokopi, Eleni Varda, Despoina Kokkinidou, Andreas Samourides, Panayiota Xylia, Pavlina Onisiforou, Menelaos Stavrinides, Nikolaos Tzortzakis, Andreas Anayiotos
Summary: This study aimed to evaluate the potential effects of an Eco-product (EP) based on rosemary and eucalyptus essential oils on crop yield and quality, as well as investigate its toxicity to animals and humans. The results showed that the use of the EP formula can increase yield and positively affect the secondary metabolism pathways of the plants.
Article
Computer Science, Interdisciplinary Applications
Huidong Xie, Zhao Liu, Luyao Shi, Kathleen Greco, Xiongchao Chen, Bo Zhou, Attila Feher, John C. Stendahl, Nabil Boutagy, Tassos C. Kyriakides, Ge Wang, Albert J. Sinusas, Chi Liu
Summary: In nuclear imaging, partial volume effects caused by limited resolution affect image sharpness and quantitative accuracy. Current anatomical-guided methods for partial volume correction require tedious image registration and segmentation steps. In this work, a deep-learning-based method for fast cardiac SPECT PVC without anatomical information and associated organ segmentation is developed. The proposed network with densely-connected dynamic mechanism produces superior results and statistically comparable measurements to anatomical-guided PVC methods, showing potential for clinical translation.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2023)
Article
Multidisciplinary Sciences
Tyra M. Pendergrass Boomer, Lily A. Hoerner, Claudia-Santi F. Fernandes, Amber Maslar, Sherry Aiudi, Tassos C. Kyriakides, Lynn E. Fiellin
Summary: This study aims to evaluate the effectiveness of a digital health game, PlaySmart, in preventing adolescent opioid misuse and improving their mental health outcomes. The study recruited 532 adolescents aged 16-19 who are at risk of opioid misuse, and they were randomized to play either PlaySmart or a set of control videogames for six weeks. Multiple assessments were conducted over a 12-month period to measure outcomes related to opioid misuse and mental health.
Article
Medicine, Legal
Matheos Giakoumi, Pavlos S. Stephanou, Konstantinos Kapnisis, Andreas Anayiotos
Summary: Contamination caused by metal ions leaching from medical device materials is a significant health problem. The use of modeling and simulation tools is recommended to support medical device submissions. An expanded physiologically based toxicokinetic model is used to predict the toxicological impact of medical devices.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Materials Science, Biomaterials
Konstantinos Kapnisis, Andreas Stylianou, Despoina Kokkinidou, Adam Martin, Dezhi Wang, Peter G. Anderson, Marianna Prokopi, Chara Papastefanou, Brigitta C. Brott, Jack E. Lemons, Andreas Anayiotos
Summary: A recent FDA report examined the scientific information on the biological response to metal implants. Using a mouse stent implantation model, the study found that surface condition and implantation time affected the inflammatory response and subsequent formation of neointima. It also indicated that elevated metal particle contamination in corroded-stent samples caused a stronger immune response.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Rebecca Slotkin, Tassos C. Kyriakides, Anupam Kundu, Gary Stack, Richard E. Sutton, Shaili Gupta
Summary: This study aims to describe the correlation between commercially available assays for anti-S1/RBD IgG and protective serum neutralizing antibodies (nAb) against SARS-CoV-2 in vaccinated adults, and determine the impact of clinical variables on this correlation. The results show a strong correlation between IgG anti-S1/RBD and nAb IC50 after vaccination, with clinical comorbidities such as prior COVID infection and renal function affecting this correlation.
Article
Medicine, Research & Experimental
Christine P. Kowalski, Linda M. Kawentel, Tassos C. Kyriakides, Lori Davis, Nicholas W. Bowersox, Amy M. Kilbourne, Grant D. Huang, Andrea L. Nevedal
Summary: Implementation assessment plans are crucial for clinical trials to ensure effective interventions are implemented after trial completion. This article introduces an Implementation Planning Assessment (IPA) Tool to guide post-trial implementation and provides a case study demonstrating its use.
JOURNAL OF CLINICAL AND TRANSLATIONAL SCIENCE
(2022)
Proceedings Paper
Engineering, Multidisciplinary
Kyriacos Themistocleous, Marinos Ioannides, George Tryfonos, Douglas Pritchard, Harriet Cliffen, Maria Katiri, Nenad Joncic, Giulia Osti, Thomas Rigauts, Francesco Ripanti, Andreas Anayiotos
Summary: HBIM was used to digitally document the Church of Panayia Karmiotissa in Cyprus, using laser scanning and photogrammetry techniques to create a 3D model and texture of the church. This is important for conservation and restoration activities.
EARTH RESOURCES AND ENVIRONMENTAL REMOTE SENSING/GIS APPLICATIONS XIII
(2022)