期刊
GENOME BIOLOGY AND EVOLUTION
卷 9, 期 3, 页码 761-776出版社
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evx042
关键词
filamentation induced by cAMP; AMPylation; parallel evolution; bacterial effector
资金
- Swiss National Science Foundation [310030B-149886, 31003A_160345]
- European Research Council Advanced Investigator Grant [FICModFun 340330]
- Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis
- American Kennel Club Canine Health Foundation
- Swiss National Science Foundation (SNF) [31003A_160345, 310030B_149886] Funding Source: Swiss National Science Foundation (SNF)
The alpha-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex effector repertoires. However, the limited availability of genome sequences from one of those lineages as well as other, remote branches of Bartonella has so far hampered comprehensive understanding of how the VirB/D4 T4SS and its effectors called Beps have shaped Bartonella evolution. Here, we report the discovery of a third repertoire of Beps associated with the VirB/D4 T4SS of B. ancashensis, a novel human pathogen that lacks any signs of host adaptability and is only distantly related to the two species-rich lineages encoding a VirB/D4 T4SS. Furthermore, sequencing of ten new Bartonella isolates from under-sampled lineages enabled combined in silico analyses and wet lab experiments that suggest several parallel layers of functional diversification during evolution of the three Bep repertoires from a single ancestral effector. Our analyses show that the Beps of B. ancashensis share many features with the two other repertoires, but may represent a more ancestral state that has not yet unleashed the adaptive potential of such an effector set. We anticipate that the effectors of B. ancashensis will enable future studies to dissect the evolutionary history of Bartonella effectors and help unraveling the evolutionary forces underlying bacterial host adaptation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据