期刊
GENERAL AND COMPARATIVE ENDOCRINOLOGY
卷 246, 期 -, 页码 301-308出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2017.01.006
关键词
Cyclic AMP; Cyclic GMP; Calcium ionophore; Litopenaeus vannamei; Recombinant sinus gland peptide; Vitellogenin
资金
- JIRCAS
- Grants-in-Aid for Scientific Research [15F15094, 15K07576] Funding Source: KAKEN
We incubated fragments of Litopenaeus vannamei ovary to investigate second messengers involved in the regulation of vitellogenin (vg) mRNA levels. The use of 100 nM recombinant vitellogenesis-inhibiting hormone (VIH) (corresponding to recombinant L. vannamei sinus gland peptide-G: rLiv-SGP-G) significantly reduced vg mRNA expression in sub-adults after 8 h incubation to less than 20% of the control. The concentration of intracellular cyclic guanosine monophosphate (cGMP) increased 3.2-fold relative to the control after 2 h incubation with rLiv-SGP-G. However, it reached levels 18-fold relative to the control after 0.5 h incubation with rLiv-SGP-G where 3-isobutyl-1-methylxanthine (a phosphodiesterase inhibitor) was also added. Moreover, vg mRNA expression was significantly reduced to less than 50% of the control after 24 h incubation with 1 mu M A23187 (a calcium ionophore). Thus, rLiv-SGP-G and calcium ionophore reduced vg mRNA expression in in vitro-cultured ovary, and cGMP may be involved in the signaling pathway of VIH. Overall, the above results suggest that vg mRNA expression might be inhibited in vitro by increasing intracellular cGMP and Ca2+ in L. vannamei ovary. (C) 2017 Elsevier Inc. All rights reserved.
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