期刊
GENE
卷 605, 期 -, 页码 70-80出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2016.12.020
关键词
Anura; Atomic force microscopy; cDNA; Circular dichroism; Surface Plasmon Resonance
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [1122012010005000]
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [1199]
- Programa Operacional Regional do Norte under QREN, through FEDER [NORTE-07-0124-FEDER-000002-Host-Pathogen Interactions]
- FCT [SFRH/BD/97995/2013, SFRH/BD/93766/2013, UID/MULTI/04378/2013]
- FCT - POPH-QREN-Tipologia 4.1-Formacao Avancada - Fundo Social Europeu and Ministerio da Ciencia, Tecnologia e Ensino Superior [SFRH/BD/97995/2013, SFRH/BD/93766/2013]
- CNPq [400398/2014-1]
- Fundação para a Ciência e a Tecnologia [UID/Multi/04378/2013, SFRH/BD/93766/2013, SFRH/BD/97995/2013] Funding Source: FCT
Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 mu M). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery. (C) 2016 Elsevier B.V. All rights reserved.
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