期刊
FOOD CHEMISTRY
卷 232, 期 -, 页码 10-18出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.03.155
关键词
Sea cucumber (Stichopus japonicus); Autolysis; Collagen fibres; Cysteine proteinases; Degradation; Interfibrillar proteoglycan bridges
资金
- National Natural Science Foundation of China [30901124, 31401562, 31401520, 31401519]
- Project of Distinguished Professor of Liaoning Province [2015-153]
- Organization Project for National Engineering Research Center of Seafood - Ministry of Science and Technology of the People's Republic of China [2012FU125X03]
Autolysis of sea cucumber, caused by endogenous enzymes, leads to postharvest quality deterioration of sea cucumber. However, the effects of endogenous proteinases on structures of collagen fibres, the major biologically relevant substrates in the body wall of sea cucumber, are less clear. Collagen fibres were prepared from the dermis of sea cucumber (Stichopus japonicus), and the structural consequences of degradation of the collagen fibres caused by endogenous cysteine proteinases (ECP) from Stichopus japonicus were examined. Scanning electron microscopic images showed that ECP caused partial disaggregation of collagen fibres into collagen fibrils by disrupting interfibrillar proteoglycan bridges. Differential scanning calorimetry and Fourier transform infrared analysis revealed increased structural disorder of fibrillar collagen caused by ECP. SDS-PAGE and chemical analysis indicated that ECP can liberate glycosaminoglycan, hydroxyproline and collagen fragments from collagen fibres. Thus ECP can cause disintegration of collagen fibres by degrading interfibrillar proteoglycan bridges. (C) 2017 Elsevier Ltd. All rights reserved.
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