Immunomodulatory effect of characterized extract of Zataria multiflora on Th1, Th2 and Th17 in normal and Th2 polarization state

The effect of the extract of Zataria multiflora (Z. multiflora) on IFN-gamma, FOXP3, IL-4, TGF-beta, and IL-17 gene expression was evaluated in cultured splenocytes obtained from control, nontreated asthma or sensitized mice (group S), Sensetized animals treated with dexamethasone or three concentrations of Z. multiflora extract (200, 400 and 800 mu g/ml) (n = 6, for each group). IFN-gamma and FOXP3 gene expressions were significantly decreased (P < 0.001 for both cases) but IL-4 (P < 0.001) and IL-17 (P < 0.05) were increased in group S compared to control group. 1 Multiflora extract 800 mu g/ml, significantly upregulated IFN-gamma gene expression (P < 0.01) and its 400 and 800 mu g/ml concentrations increased FOXP3 gene expression (P < 0.05 and P < 0.001, respectively) compared to group S. Z. multiflora extract at all concentrations (200, 400 and 800 mu g/ml) decreased TGF-beta gene expression and its lowest concentration significantly reduced IL-17 gene expression compared to group S (P < 0.001 for all cases). Only IL-4 and TGF-beta gene expression was significantly decreased following treatment with dexamethasone (P < 0.001 for both cases). The results indicated an increase in IFN-gamma and FOXP3 but decrease in TGF-beta and IL-17 gene expression profile in sensitized splenocytes treated with the extract, which might be partially due to the presence of one of its constituent, carvacrol. (C) 2016 Published by Elsevier Ltd.