Article
Immunology
Valerie Cornet, Trinh Dinh Khuyen, Syaghalirwa N. M. Mandiki, Stephane Betoulle, Peter Bossier, Felipe E. Reyes-Lopez, Lluis Tort, Patrick Kestemont
Summary: Beta-glucans, prebiotic and/or food additives used in aquaculture, have varying effects on fish immune response and disease resistance. Gas1 beta-glucan may be more efficient in enhancing immunity and preventing diseases compared to other types already in use.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
M. Camino Ordas, Lucia Gonzalez-Torres, Paula Arense, Rebecca Heavyside, Carlos Zarza, Carolina Tafalla
Summary: This study investigated the effects of beta-glucans on rainbow trout using the RTS11 and RTgutGC cell lines. The results showed that RTS11 monocytes/macrophages were more reactive to beta-glucans, while beta-glucans also had immunostimulatory effects on gut epithelial cells. Additionally, beta-glucans were found to induce tolerance in both cell lines, with only interleukin 6 transcription significantly lower in cells previously exposed to beta-glucans.
Article
Fisheries
Felix Docando, Paula Arense, Alba Martin-Martin, Tiehui Wang, Carolina Tafalla, Patricia Diaz-Rosales
Summary: Disease prevention through vaccination is the most appropriate method for pathogen control in aquaculture, but oral vaccines face technical problems in aquatic animals. This study explores different molecules as oral adjuvants and finds that LPS shows strong immunostimulatory potential and could be safely used as a mucosal adjuvant in rainbow trout.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Fabian Ricardo Gomez de Anda, Vicente Vega-Sanchez, Nydia Edith Reyes-Rodriguez, Victor Manuel Martinez-Juarez, Juan Carlos Angeles-Hernandez, Ismael Acosta-Rodriguez, Rafael German Campos-Montiel, Andrea Paloma Zepeda-Velazquez
Summary: In this study, rainbow trout fingerlings were experimentally infected with different Aeromonas species, and the behavior and histopathology of the infected groups were observed and recorded. The findings indicate that different Aeromonas species cause varying behavioral changes and histopathological manifestations in infected fish.
APPLIED SCIENCES-BASEL
(2021)
Article
Fisheries
Caren Arteaga Quico, Mauro Mariano Astocondor, Ronald Aquino Ortega
Summary: This study demonstrated that Chlorella peruviana supplementation has significant effects on the growth, physiological parameters, and innate immune system of rainbow trout fingerlings, with optimal supplementation levels between 3.6 and 5.0 g kg(-1).
Article
Fisheries
Fidel Herrera, Janet Velazquez, Juana Maria Lugo, Paola Orellana, Joceline Ruiz, Matias Vega, Alex Romero, Nestor Santos, Gerardo Ramses, Tania Rodriguez-Ramos, Brian Dixon, Mario Pablo Estrada, Patricio Dantagnan, Yamila Carpio
Summary: This study demonstrated that dietary supplementation with synthetic PACAP-38 can improve PUFA content in muscle, increase the number of caliciform cells within intestinal villi, and modulate the transcription of cytokines in head kidney of rainbow trout fingerlings. These findings suggest potential benefits of using small peptides as feed additives to enhance the quality of cultured fish fingerlings.
AQUACULTURE REPORTS
(2021)
Review
Fisheries
Yongji Dang, Yanling Sun, Yangen Zhou, Xianhui Men, Bowen Wang, Bing Li, Yichao Ren
Summary: This study evaluated the effects of probiotics on growth, immune response, and susceptibility to bacterial infection in rainbow trout. The results showed that probiotics promoted weight gain and resistance to bacterial infection in rainbow trout. Different Toll-like receptors were involved in the recognition of beneficial bacteria, and probiotics enhanced the innate immune function of rainbow trout by training immune response pathways.
Article
Fisheries
Rebecca A. Dupre, Ryan Ardoin, Jesse Trushenski, Chris Jackson, Casey Grimm, Brennan Smith
Summary: The present research demonstrates that dietary exposure is an alternative route of geosmin uptake in Rainbow Trout fillets, leading to a significant increase in geosmin concentrations. This finding is important for further investigation of sensory quality and off-flavor mitigation strategies for farm-raised fish.
Article
Fisheries
Hafsan Hafsan, Dmitry Bokov, Walid Kamal Abdelbasset, Mustafa M. Kadhim, Wanich Suksatan, Hasan Sh Majdi, Gunawan Widjaja, Abduladheem Turki Jalil, Maytham T. Qasim, Mahboubeh Balvardi
Summary: This study investigated the effects of Dracocephalum kotschyi essential oil (DKE) on the growth, immune and antioxidant defense system, and resistance to bacterial infection in rainbow trout juveniles. The results showed that the DKE supplementation improved the immune response, antioxidant enzyme activity, and survival rate of the fish.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Yehfang Hu, Ayham Alnabulsi, Abdo Alnabulsi, Callum Scott, Carolina Tafalla, Christopher J. Secombes, Tiehui Wang
Summary: IFN-gamma is a key cytokine in Th1 immune responses, playing a crucial role in protection against infections. Monoclonal antibodies developed in this study were able to monitor IFN-gamma expression, revealing that various cells in trout can produce IFN-gamma and increase following infection or vaccination. Monitoring IFN-gamma production may be important in assessing the effectiveness of Th1 responses and cell mediated immunity in fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Magdalena Malachowicz, Andrzej K. Siwicki, Stefan Dobosz, Roman Wenne
Summary: In this study, the transcriptional changes in the skin of rainbow trout in response to a bacterial infection were investigated using oligonucleotide microarray technology. The study identified many differentially expressed genes involved in immune response and revealed the connection between metabolic remodeling and immune response.
APPLIED SCIENCES-BASEL
(2023)
Article
Fisheries
Chu Zeng, Zhi-Shuai Hou, Hong-Kui Zhao, Yuan-Ru Xin, Meng-Qun Liu, Xiao-Dong Yang, Hai-Shen Wen, Ji-Fang Li
Summary: In rainbow trout, 18 caspase genes were identified, with different roles observed in response to Aeromonas salmonicida and Vibrio anguillarum infections. CASP3-regulated intrinsic apoptosis was activated after A. salmonicida infection, while CASP8 and CASP6-regulated extrinsic apoptosis had the greatest effect on trout challenged with V. anguillarum. RNA-Seq data showed the integration of casp8 with significantly enriched Gene Ontology terms and functional pathways in response to V. anguillarum infection, including apoptosis regulation, pathogen detection, and immunomodulation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Mohamed Omar Abdalla Salem, Yigit Tastan, Soner Bilen, Ertugrul Terzi, Adem Yavuz Sonmez
Summary: This study investigated the effects of flaxseed oil as a feed additive on the growth, oxidative stress, immunity, and disease resistance of rainbow trout. The results showed that the addition of flaxseed oil increased the final weight and specific growth rate of the fish, as well as their resistance to a specific bacteria. Furthermore, flaxseed oil improved the immune response and reduced oxidative damage in the fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yongji Dang, Chunyan Zhao, Kok Chee Steven Kuan, Xiyu Cao, Bowen Wang, Yichao Ren
Summary: This study evaluated the effects of oregano essential oil (OEO) on the intestinal microbiota, immunity, growth, amino acid composition, and resistance to Aeromonas salmonicida in rainbow trout. The results showed that OEO supplementation had positive effects on the growth and pathogen resistance of rainbow trout, with higher amino acid deposition in the muscle and significant enrichment in amino acid pathways. OEO supplementation led to changes in the abundance of beneficial and potential pathogenic bacteria in the intestinal microbiota. Transcriptome analysis revealed the up-regulation of immune-related genes and down-regulation of certain cytokines. Overall, OEO intervention improved amino acid deposition and resistance to A. salmonicida infection in rainbow trout by modulating the link between fish immunity and intestinal microbiota function.
AQUACULTURE INTERNATIONAL
(2023)
Article
Microbiology
Baptiste Redivo, Nicolas Derome, Patrick Kestemont, Valerie Cornet
Summary: Environmental stressors can disrupt the microbiota-host relationship and cause loss of function. In this study, the effects of Aeromonas salmonicida infection on the taxonomic composition and structure of the fish microbiota were assessed. The results indicate that the infection can lead to dysbiosis and an increase in opportunistic pathogens, and the findings may contribute to the development of non-invasive disease prevention techniques in aquaculture.
Article
Engineering, Environmental
I. Brandts, C. Barria, M. A. Martins, L. Franco-Martinez, A. Barreto, A. Tvarijonaviciute, L. Tort, M. Oliveira, M. Teles
Summary: This study evaluated the short-term exposure of 45 nm PMMANPs on gilthead seabream by analyzing biomarkers in liver and plasma. The results showed an up-regulation of genes associated with lipid metabolism, an increase in cholesterol and triglycerides in plasma, and activation of antioxidant defenses. After 96 hours, the antioxidant capacity decreased and genotoxicity in blood cells was observed.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Immunology
Felipe E. Reyes-Lopez, Antoni Ibarz, Borja Ordonez-Grande, Eva Vallejos-Vidal, Karl B. Andree, Joan Carles Balasch, Laura Fernandez-Alacid, Ignasi Sanahuja, Sergio Sanchez-Nuno, Joana P. Firmino, Leonardo Pavez, Javier Polo, Lluis Tort, Enric Gisbert
Summary: This study analyzed the response of gilthead sea bream skin mucosa to dietary inclusion of spray-dried porcine plasma using a holistic multi-omics approach. The interaction between skin tissue and exuded mucus was found to improve tissue development, innate defenses, and environment recognition. The results emphasize the importance of crosstalk between skin tissue and mucus in mucosal functionality.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Joana P. Firmino, Laura Fernandez-Alacid, Eva Vallejos-Vidal, Ricardo Salomon, Ignasi Sanahuja, Lluis Tort, Antoni Ibarz, Felipe E. Reyes-Lopez, Enric Gisbert
Summary: Dietary supplementation of garlic essential oil, carvacrol, and thymol in gilthead seabream juveniles modulated skin transcriptional immune response, bacterial growth capacity in skin mucus, and overall health condition. The supplementation promoted innate immunity and mucus protective capacity, reducing susceptibility to pathogenic bacteria colonization.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Veterinary Sciences
Juan Ramos, Joan Carles Balasch, Lluis Tort
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Ricardo Salomon, Felipe E. Reyes-Lopez, Lluis Tort, Joana P. Firmino, Carmen Sarasquete, Juan B. Ortiz-Delgado, Jose C. Quintela, Jose M. Pinilla-Rosas, Eva Vallejos-Vidal, Enric Gisbert
Summary: The study showed that the inclusion of medicinal plant leaf extract from sage and lemon verbena in the diet of gilthead seabream had beneficial effects on gut health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Ingrid Camargo dos Reis, Camino Fierro-Castro, Giovanni Sampaio Gonsalves, Basia Schlichting Moromizato, Lluis Tort, Jaqueline Dalbello Biller
Summary: The study demonstrates that the combination of vaccines with immunostimulants like beta-glucan can enhance immune response in fish. The upregulation of immune-related genes in the head kidney and spleen, along with increased expression of HSP70, suggests a more robust defense against diseases. This indicates that beta-glucan may stimulate the immune response through DAMPs recognition, promoting a stronger immune defense.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Correction
Microbiology
Carla Hernandez-Cabanyero, Eva Sanjuan, Felipe E. Reyes-Lopez, Eva Vallejos-Vidal, Lluis Tort, Carmen Amaro
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Carla Hernandez-Cabanyero, Eva Sanjuan, Felipe E. Reyes-Lopez, Eva Vallejos-Vidal, Lluis Tort, Carmen Amaro
Summary: This study investigates the mechanisms behind fish death caused by Vibrio vulnificus, a zoonotic pathogen associated with fish farms. The researchers found evidence of an atypical inflammatory response in fish, characterized by two phases, triggered by V. vulnificus infection. They also identified the role of red blood cells (RBCs) in this immune response. The findings of this study provide insights into the pathogenesis of V. vulnificus infection in both fish and humans.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Physiology
Irene Garcia-Meilan, Lluis Tort, Ali Reza Khansari
Summary: The study aimed to determine the integrated stress response in rainbow trout after acute hypoxia. The results showed that physiological responses were significantly affected by hypoxic stress and their interaction with time after the challenge. The study also revealed the importance of different elements and areas of the stress response, including systemic and mucosal responses, in maintaining homeostasis.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Fisheries
Camino Fierro-Castro, Josep Rotllant, Jose Miguel Cerda-Reverter, Lluis Tort
Summary: The melanocortin system is an ancient neuroendocrine system involved in various physiological processes. This study found that zebrafish lacking the MC1R receptor showed lower cortisol levels and reduced macrophages in response to LPS stimulation. The expression of anti-inflammatory genes was also increased in the mutant fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biodiversity Conservation
M. Blonc, I. Brandts, M. Canovas, L. Franco-Martinez, C. P. Rubio, L. Tort, A. Tvarijonaviciute, C. Gravato, M. Teles
Summary: The present study aimed to validate a series of automated assays to monitor the effects of chronic exposure to nanoplastics on fish. Adult goldfish were exposed to 100 μg/L polystyrene nanoparticles (PS-NPs) for 30 days. The results showed that NPs triggered immune and antioxidant responses in the gills, but had no significant effect on the brain and muscle. These findings suggest that gills may be the primary target for NPs and further investigation is needed to understand the cascading effects on gas exchange and osmo-ionic regulation.
ECOLOGICAL INDICATORS
(2023)
Article
Fisheries
Merari Goldstein, Eva Vallejos-Vidal, Valentina Wong-Benito, Felipe Barraza-Rojas, Lluis Tort, Felipe E. Reyes-Lopez, Monica Imarai
Summary: This study investigated the effects of artificial photoperiods on the leukocyte populations and T helper responses in rainbow trout. The results showed that photoperiod induced changes in head kidney leukocyte subsets and T cell gene expression. Different photoperiods led to unique immune signatures of antigenic response and impacted immune memory and protection against microorganisms in rainbow trout.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Agriculture, Dairy & Animal Science
Manuel Blonc, Jennifer Lima, Joan Carles Balasch, Lluis Tort, Carlos Gravato, Mariana Teles
Summary: Pharmaceuticals used to treat abnormal cholesterol levels, known as lipid regulators, are increasingly being used and are causing concerns in aquatic systems. The present review examines the effects of these medications on finfish species, finding that both short- and long-term exposure to these drugs can negatively impact fish health and reproduction. However, more research is needed to fully understand the implications for aquaculture and human health. The most studied lipid regulators include gemfibrozil, clofibrate, atorvastatin, and simvastatin.
Article
Fisheries
Simone Andrea de Assis Beneti, Ingrid Camargo dos Reis, Camino Fierro-Castro, Basia Schlichting Moromizato, Gustavo do Valle Polycarpo, Celso Tadao Miasaki, Jaqueline Dalbello Biller
Summary: This study evaluated the effect of beta-glucan on gene transcription mechanisms via the Tlr-Myd88-Nfkb1 pathway in Nile tilapia under stress. The results showed that beta-glucan, combined with stressful management, modulated the immune system and promoted early defense in fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jaqueline Dalbello Biller, Gustavo do Valle Polycarpo, Basia Schlichting Moromizato, Ana Paula Diogo Sidekerskis, Thais Daltoso da Silva, Ingrid Camargo dos Reis, Camino Fierro-Castro
Summary: This study aimed to describe a protocol for evaluating lysozyme activity in tilapia, comparing the effects of lipopolysaccharide and Streptococcus agalactiae injections. The findings indicated that a concentration of 250 μg kg(-1) LPS promoted immunomodulation similar to challenge with S. agalactiae, showcasing standardized analysis methods for future studies.
REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE
(2021)