期刊
FISH & SHELLFISH IMMUNOLOGY
卷 63, 期 -, 页码 255-260出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.02.019
关键词
Streptococcus agalactiae; Tilapia; GapA; Vaccine
资金
- Natural Science Foundation of China [31572657, 31372563, 31560725]
- Fundamental Research Funds for the Central Universities [2014PY035]
- Special Fund for Science and Industry Development from the Administration of Ocean and Fisheries of Guangdong Province [A201512C003]
- Special Fund for Fish Disease Prevention and Therapy from Guangdong Province [2015-115]
- Special Fund for Science and Technology from Hubei Province [2015BBA228]
- Innovative Research Team Project of the Hainan Natural Science Foundation [2016CXTD005]
Streptococcosis due to the bacterium Streptococcus agalactiae (S. agalactiae) has resulted in enormous economic losses in aquaculture worldwide, especially in the tilapia culture industry. Previously, there were limited vaccines that could be employed against streptococcosis in tilapia. This study aimed to develop a vaccine candidate using the glyceraldehyde-phosphate dehydrogenase protein (GapA) of S. agalactiae encoded by the gapA gene. Tilapia were intraperitoneally injected with PBS, PBS + Freund's adjuvant, PBS + Montanide's adjuvant, GapA + Freund's adjuvant, GapA + Montanide's adjuvant, killed S. agalactiae whole cells (WC)+Freund's adjuvant, or killed S. agalactiae whole cells (WC)+ Montanide's adjuvant. They were then challenged with S. agalactiae, and the relative percentage survival (RPS) was monitored 14 days after the challenge. The highest RPSs were observed in the WC groups, with 76.7% in WC + Freund's adjuvant and 74.4% in WC + Montanide's adjuvant groups; these were followed by the GapA groups, with 63.3% in GapA + Freund's adjuvant and 45.6% in GapA + Montanide's adjuvant groups. The RPS of the PBS group was 0%, and those of PBS + Freund's adjuvant and PBS + Montanide's adjuvant groups were 6.7% and 3.3%, respectively. Additionally, the IgM antibody responses elicited in GapA groups and WC groups were significantly higher than those in PBS groups. Furthermore, the expressions of cytokine (IL-1 beta and TNF-alpha) mRNAs in the GapA groups and WC groups were significantly higher than those in the PBS groups. Taken together, these results reveal that the GapA protein is a promising vaccine candidate that could be used to prevent streptococcosis in tilapia. (C) 2017 Elsevier Ltd. All rights reserved.
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