Article
Fisheries
Hongyan Yu, Xuewen Jia, Yifan Pang, Huiqin Niu, Biao Du, Xiaoyan Xu, Jiale Li
Summary: In this study, it was found that the TBK1 gene in grass carp could be upregulated by bacterial infection. Overexpression of TBK1 could reduce the number of adhesive bacteria in cells and promote cellular migration, proliferation, vitality, and anti-apoptosis ability. TBK1 could activate the NF-kappa B signaling pathway and induce autophagy in cells. These findings provide important information about the defense and immune mechanisms used by grass carp against pathogens.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yanan Li, Xiaolong Qiu, Zhijie Lu, Fanbin Zhan, Minxuan Yang, V. Sarath Babu, Jun Li, Zhendong Qin, Li Lin
Summary: In this study, the MST2 gene of grass carp was cloned and its tissue distribution was analyzed. CiMST2 was highly expressed in brain tissue and significantly up-regulated under bacterial infection, indicating its involvement in the immune response. CiMST2 regulated host redox homeostasis via Nrf2-ARE signaling, providing insights for disease prevention and control in the grass carp farming industry.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Immunology
Nan Wang, Jiahao Li, Yajun Wang, Yingying Wang, Defeng Zhang, Cunbin Shi, Yingying Li, Sven M. Bergmann, Xubing Mo, Jiyuan Yin, Qing Wang
Summary: In this study, Lactococcus lactis was used as an oral vaccine delivery system to express the VP6 protein of grass carp reovirus II (GCRV II). It was found that the recombinant L. lactis strain could induce mucosal immune protection, enhance nonspecific immune responses, and elicit both systemic and mucosal immunity in fish. This study suggests that L. lactis is a promising candidate for oral vaccine delivery in the aquaculture industry.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
De-Kui Qiu, Yan Xiong, Yu-Ming Gong, Yu-Ying Zheng, Rui Ma, Chen Zhang, Gao-Xue Wang, Bin Zhu
Summary: The study found that grass carp vaccinated with pcDNA3.1-vp4 showed better immune responses compared to pcDNA3.1-vp4-3, with the latter having a higher survival rate in preventing GCRV infection.
Article
Microbiology
Fanshu Xiao, Lanjie Liao, Qiaoqing Xu, Zhili He, Tiaoyi Xiao, Jianjun Wang, Jie Huang, Yuhe Yu, Bo Wu, Qingyun Yan
Summary: This study observed a dysbiosis of gut microbiota in grass carp infected with the GCRV virus, with an overabundance of Cetobacterium somerae triggering exacerbated inflammatory responses through NLRs, TLRs, and LPS pathways.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Fisheries
Lei Zhu, Gaoliang Yuan, Xinru Wang, Tong Zhao, Libo Hou, Chen Li, Xinyu Jiang, Jie Zhang, Xianliang Zhao, Chao Pei, Li Li, Xianghui Kong
Summary: In this study, a vaccine delivery system using duckweed as a host for the expression of the S11 segment of grass carp reovirus (GCRV) was successfully constructed. The expression of the S11 segment in transgenic duckweed was verified using various methods, including GUS staining, PCR, Western blotting, and fluorescence observation. The results showed that the expression vector was successfully transferred and replicated in duckweed, and a fusion protein with a molecular mass of approximately 50 kDa was expressed. This study provides valuable information for the development of an edible vaccine against GCRV in grass carp.
Article
Fisheries
Fanbin Zhan, Yanan Li, Fei Shi, Zhijie Lu, Minxuan Yang, Qingqing Li, Li Lin, Zhendong Qin
Summary: This study identified two forms of TLR5 (TLR5a and TLR5b) in grass carp and investigated their role in innate immunity against flagellate and non-flagellate bacteria. It was found that TLR5b had higher expression levels and stronger response to pathogen stimulation compared to TLR5a. The results provide insight into the role of TLR5 in fish infection resistance and contribute to the research on innate immunity in grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Ting Gao, Caixia Gao, Siyu Wu, Yingying Wang, Jiyuan Yin, Yingying Li, Weiwei Zeng, Sven M. Bergmann, Qing Wang
Summary: The study successfully developed GCRV II-based virus-like particles (VLPs) using a bac-to-bac expression system, which demonstrated excellent immunogenicity and showed protective effects in grass carp after immunization.
Article
Fisheries
Xiaoqing Xiong, Hong Yang, Chunhua Ding, Beibei Qin, Yadong Deng, Liming Xiong, Xiaoyan Liu, Yaoguo Li, Tiaoyi Xiao, Zhao Lv
Summary: In this study, a newly discovered integrin αvβ3 in grass carp was found to interact with viral proteins and mediate antiviral immune response. Furthermore, the expression of this integrin was significantly upregulated in various tissues of grass carp after infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xinyu Liang, Yongming Li, Pengfei Chu, Qian Wang, Hanyue Wang, Lanjie Liao, Cheng Yang, Zuoyan Zhu, Yaping Wang, Libo He
Summary: This study cloned and analyzed the peroxiredoxin 3 gene from grass carp and found that it can enhance host antioxidant activity and induce autophagy to inhibit the replication of grass carp reovirus.
Article
Fisheries
Yanlin Dai, Yiqun Li, Ge Lin, Jingjing Zhang, Nan Jiang, Wenzhi Liu, Yan Meng, Yong Zhou, Yuding Fan
Summary: This study investigated the cell death mechanisms involved in grass carp reovirus - HH196 (GCRV-HH196) infection using a grass carp ovary cell line (GCO cells). Results showed that GCRV-HH196 infection caused DNA damage, cell volume reduction, and cytoplasm shrinkage. The expression levels of pro-apoptotic genes were up-regulated and caspase proteins involved in apoptosis were activated. Flow cytometry analysis revealed a higher number of apoptotic cells in GCRV-HH196-infected cells compared to control cells. Additionally, autophagy was also involved in the regulation of GCRV-HH196 infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Fufa Qu, Jialing Li, Xuan Zeng, Qing She, Yurong Li, Wenjie Xie, Shenping Cao, Yonghua Zhou, Zhimin He, Jianzhou Tang, Zhuangwen Mao, Yuping Wang, Jiamei Fang, Wenqian Xu, Zhen Liu
Summary: The study identified a fish MAP3K4 homolog in grass carp and revealed its crucial role in intestinal immune responses following bacterial infection, shedding light on the understanding of the intestinal immune system in fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
De-Kui Qiu, Yi-Jun Jia, Yu-Ming Gong, Yu-Ying Zheng, Gao-Xue Wang, Bin Zhu
Summary: The study aimed to optimize the immunization program of SWCNTs-vaccine controlling infectious GCRV in grass carp, and it found that 12 hours of immunization time, 10 mg L-1 of antigen concentration, and 15 fish per liter of fish density resulted in the highest relative percent survival (88.33%), indicating the highest vaccine effect against the disease.
Article
Fisheries
Yu Chen, Mengjing Zhao, Xiongwei Fan, Ping Zhu, Zhaobiao Jiang, Faxiang Li, Wuzhou Yuan, Shiqi You, Jimei Chen, Yunxuan Li, Yan Shi, Xiaolan Zhu, Xiangli Ye, Fang Li, Jian Zhuang, Yongqing Li, Zhigang Jiang, Yuequn Wang, Xiushan Wu
Summary: The expression of gcFKBP5 in the primary innate immune organs of grass carp was highly upregulated by SVCV within 24 hours, as well as in fish cells treated with poly(I:C). Overexpression of gcFKBP aggravated viral damage to cells and increased viral replication. Additionally, SVCV interacts with gcFKBP5 and TRAF2 to promote host cell apoptosis for supporting viral replication.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Wei-Chao Chen, Er-Long Wang, Fei Luo, Kai-Ge Song, Gao-Xue Wang
Summary: A new strain of SVCV named SVCV-CG01 was isolated from diseased zebrafish and its genome characteristics were determined. The virus belongs to the Ia genogroup, shows high pathogenicity, and can induce mass fish mortality.
Article
Fisheries
Kaige Song, Fei Luo, Weichao Chen, Xiaozhou Qi, Yufeng Shen, Jiwei Zha, Fei Ling, Gaoxue Wang
Summary: The ethyl acetate extract from Bacillus velezensis JW supernatant (EJW) showed highly efficient anti-SVCV activity both in vitro and in vivo, inhibiting early events of SVCV replication and increasing the survival rate of infected common carp. Furthermore, EJW up-regulated the expression of antiviral immune genes, indicating its potential as a promising antiviral strategy against SVCV.
Article
Fisheries
Shen-Ye Qu, Xiao-Ping Tan, Bing-Ke Li, Jie-Tao Liu, Gao-Xue Wang, Fei Ling
Summary: This study reports the first record of Ichthyophthirius multifiliis infection in Micropterus salmoides. Malformed fish suffer from more serious Ichthyophthiriasis compared to normal fish. Weak immune response and more tissue damage in malformed fish increase susceptibility to pathogens.
Article
Fisheries
Guang-Lu Liu, Ding Li, Wei-Wu Song, Li-Li Zhu, Jun-Wei Han, Yi-Wen Li, Chun-Jie Wang, Gao-Xue Wang, Ya-Hong Chen
Summary: By establishing 3D-QSAR models, this study successfully designed novel coumarin derivatives with improved antiparasitic activities, which showed promising potential in controlling Dactylogyrus infection with high stability in aquacultural water.
Article
Fisheries
Zhao Zhang, Haibo Cui, Zhiqiang Zhang, Shenye Qu, Gaoxue Wang, Fei Ling
Summary: The study investigated the pharmacokinetics of magnolol in goldfish following different administration routes and found that oral administration of magnolol effectively controlled Ichthyophthirius multifiliis infection. The results indicated that magnolol could be a potential treatment for fish infections caused by I. multifiliis.
Article
Fisheries
Wei-Chao Chen, Fei Luo, Kai-Ge Song, Gao-Xue Wang
Summary: This study further investigated the antiviral activity and mechanism of a compound called 6A against Spring viraemia of carp virus (SVCV). The results showed that 6A could increase the survival rate of zebrafish infected with SVCV and inhibit the gene expression of SVCV in the brain, spleen, and liver. Additionally, 6A exhibited a strong antiviral response in the early stage of viral infection. Therefore, 6A is expected to be a potential therapeutic agent for the treatment of SVCV in aquaculture.
Article
Fisheries
Wei-Chao Chen, Rui Ma, Tao Wang, Gao-Xue Wang
Summary: This study explored the recombinant expression of spring viraemia of carp virus glycoprotein using four different expression systems. The results showed that E. coli was the optimal expression system, but faced challenges such as the formation of insoluble aggregates. Yeast GS115 and insect Sf9 cells were also used and successfully produced the glycoprotein.
Article
Fisheries
Zhao Zhao, Yang Li, Guo Chen, Chen Zhang, Gao-Xue Wang, Bin Zhu
Summary: Infectious spleen and kidney necrosis virus (ISKNV) has caused significant economic losses on mandarin fish in China due to its wide host range and high mortality rate. Vaccines are considered one of the most effective measures to control virus infection as there are no commercial drugs available for curing ISKNV. However, the weak immunogenicity of antigen protein in subunit vaccines often requires the use of boosters, adjuvants, or carriers to ensure strong and long-lasting protective immunity. This study demonstrated that using single-walled carbon nanotubes (SWCNTs) as carriers and mannose (M) as targeting factors in a main capsid protein (MCP) subunit vaccine (SWCNTs-M-MCP) resulted in rapid and strong antibody responses, significantly higher than those of the MCP and SWCNTs-MCP groups. These findings suggest that SWCNTs and mannose can enhance the immune response of vaccines and may provide an effective strategy against ISKNV.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Song Zhu, Bo Miao, Yu-Zhou Zhang, De-Shou Wang, Gao-Xue Wang
Summary: Outbreaks of viral encephalopathy and retinopathy severely devastate aquaculture worldwide, with nervous necrosis virus (NNV) being the causative agent. Research suggests that amantadine may be a promising therapeutic agent against NNV, providing an effective strategy for controlling VER in fish at early developmental stages.
JOURNAL OF FISH DISEASES
(2022)
Article
Virology
Yingjie Jin, Fei Yang, Gengrong Zhang, Qing Yu, Gaoxue Wang, Fei Ling, Tianqiang Liu
Summary: The researchers successfully increased the anti-MSRV activity of magnolol by hydrogenating it, providing valuable references for the discovery and development of anti-MSRV drugs in aquaculture.
Article
Fisheries
Yihang Liu, Xiaopin Tan, Yiling Zhang, Fei Ling, Tianqiang Liu, Gaoxue Wang
Summary: The study found that isoimperatorin from Angelica dahurica showed significant efficacy in eradicating Gyrodactylus kobayashii in goldfish, reducing infection prevalence and intensity without causing significant toxicity to the host. Isoimperatorin damaged the tegument of G. kobayashii and disrupted its homeostasis, providing insufficient energy for the parasite, which led to promising anthelmintic effects.
Article
Fisheries
Ai-Guo Huang, Cheng Chen, Tian-Qiang Liu, Gao-Xue Wang
Summary: In this study, a novel WSSV-targeting drug delivery system SWCNTs-GPA-P75E8 was constructed, which showed stronger anti-WSSV activity by inhibiting viral replication and reducing mortality in infected crayfish.
Article
Fisheries
Linhan Li, Tao Zhang, Gengrong Zhang, Guoqing Zhou, Fei Yang, Erlong Wang, Tianqiang Liu, Gaoxue Wang
Summary: This study demonstrates that using naturally biodegradable bacterial nanocellulose (BNC) as a virus antigen carrier for bath vaccination can improve the immune response of largemouth bass. Bath immunization with BNC-loaded glycoprotein (BNCs-G) results in significantly increased serum antibody levels and expression of immune-related genes.
