期刊
FISH & SHELLFISH IMMUNOLOGY
卷 71, 期 -, 页码 76-82出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.09.064
关键词
Hepatic steatosis; Inflammation; Sirt1; NF-kappa B; Large yellow croaker
资金
- National Science Fund for Distinguished Young Scholars of China [31525024]
- National Natural Science Foundation of China [31372541, 31172425]
- AoShan Talents Program [2015ASTP]
Hepatic steatosis induced inflammation is becoming increasingly prevalent in farmed fish. This study was conducted to investigate the protective effects of omega-3 polyunsaturated fatty acids omega-3 PUFAs) against hepatic steatosis-induced inflammation and its potential molecular mechanisms in hepatocyte of large yellow croaker (Larmichthys crocea). We found that the hepatic steatosis-induced inflammation was relieved by omega-3 PUFAs, meanwhile, the Sirt1 activity and transcript expression was increased by omega-3 PUFAs. The increased Sirtl activity can decrease the hepatic steatosis-induced inflammation. The protective effects of omega-3 PUFAs against hepatic steatosis-induced inflammation was reversed by the treatment with Sirtl inhibitor EX-527. The nuclear translocation of nuclear transcription factor kappa-B (NF-kappa B) p65 was significantly decreased after omega-3 PUFAs treatments compared to the palmitic acid stimulation group. The omega-3 PUFAs induced cytoplasm translocation of NF-kappa B p65 was reversed by EX-527. Together, omega-3 PUFAs alleviate hepatic steatosis-induced inflammation through Sirtl-mediated nuclear translocation of NF-kappa B p65 subunit in hepatocytes of large yellow croaker. The present study provides important insight into the mechanisms of the protective effects of omega-3 PUFAs, providing theory bases for alleviating the hepatic steatosis induced inflammation of farmed fish, thereby offering great benefits to the aquaculture industry and fish consumers. (C) 2017 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据