Article
Fisheries
Ying Huang, Ruixia Wang, Tianheng Gao, Ting Wu, Qiya Zhang, Yangbai Shi, Shuyan Ding, Zhe Zhao
Summary: This study used RNA-seq to investigate the transcriptome response of Siniperca chuatsi to SCRV infection, revealing key genes involved in immune response. Results showed that SCRV infection induced significant changes in the immune system, cellular processes, cell growth and death, highlighting the importance of innate and adaptive immunity in the response to pathogens.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
YuHui He, Xu-Fang Liang, WenJie Guo, ChangXu Tian, LongFang Sun, Kang Huang, Shan He, YanPeng Zhang
Summary: This study found that the swimbladder inflation rate of mandarin fish increases with age, with 5-11 days post hatch being the main inflation stage. Fish larvae with inflated swimbladders were heavier than those without, and non-inflation significantly affected buoyancy maintenance and live bait predation in mandarin fish.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Shiyan Liu, Chong Han, Jingjun Huang, Qiaoying Zhu, Dingrui Liu, Linqiang Han, Shuisheng Li, Guifeng Li, Haoran Lin, Yong Zhang
Summary: Four X chromosome-specific markers were identified and validated by genome sequencing and PCR, promoting the development of all-male breeding of mandarin fish.
Article
Fisheries
Xiaoyu Liu, Xiaodong Zhang, Zhendong Xu, Zhiyang Huang, Junyao Zhong, Yinzhi Zhu, Yongwei Wei
Summary: In this study, a mandarin fish rhabdovirus S3 was isolated, and its genetic characteristics and infectivity were investigated. S3 showed the ability to infect fish-derived, mammalian, and human-derived cells, with higher infectivity at 28 degrees C. However, S3 was unable to infect cells at 37 degrees C. The study also demonstrated that S3 exhibited strong pathogenicity to zebrafish, making them a suitable infection model for SCRV.
Article
Genetics & Heredity
Weidong Ding, Xinhui Zhang, Xiaomeng Zhao, Wu Jing, Zheming Cao, Jia Li, Yu Huang, Xinxin You, Min Wang, Qiong Shi, Xuwen Bing
Summary: The high-quality genome assembly of the mandarin fish provides new insights into its special feeding habit of live prey, revealing significant gene mutations related to the olfactory and visual systems. Additionally, sex-related genes and quantitative trait loci were identified on chromosomes, contributing to the understanding of genetic mechanisms underlying the feeding behavior of this economically important species.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Jia-Ming Zou, Qiang-Sheng Zhu, Hui Liang, Hai-Lin Lu, Xu-Fang Liang, Shan He
Summary: Regulation of food intake is closely associated with nutrient-sensing systems and the expression of appetite neuropeptides. In this study conducted on mandarin fish, lysine and methionine were found to be feeding-stimulating amino acids, and neuropeptide Y (NPY) played a significant role in the regulation of food intake. Lysine deprivation activated the GCN2 signaling pathway, leading to the transcriptional induction of npy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Shuang Liu, Peng Xu, Xuange Liu, Dingli Guo, Xiaoli Chen, Sheng Bi, Han Lai, Gongpei Wang, Xiaopin Zhao, Yuqing Su, Huadong Yi, Yong Zhang, Guifeng Li
Summary: The research shows that the use of dietary 17 alpha-methyltestosterone (MT) treatment can successfully achieve all-male populations of mandarin fish, without affecting growth and survival rates, but initially inhibits the development of testes and serum hormone levels.
Article
Chemistry, Applied
Yingying Shen, Yanyan Wu, Yueqi Wang, Laihao Li, Chunsheng Li, Yongqiang Zhao, Shaoling Yang
Summary: This study used advanced techniques to investigate the flavor components and microbial diversity during the fermentation of Mandarin fish. It identified the main flavor components and three microbial genera closely associated with their production. These microorganisms play important roles in the formation of characteristic flavor substances.
Article
Fisheries
Haofeng Ouyang, Chong Han, Qiaoying Zhu, Liangqing Xu, Jingjun Huang, Shuisheng Li, Guifeng Li, Haoran Lin, Yong Zhang
Summary: The study revealed that ER beta 1, ER beta 2, and AR beta play critical roles in spermatogenesis and masculinization, while ER beta 2 and ER beta 1 are essential for ovary development and vitellogenesis in females.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Chong Han, Chongwei Wang, Haofeng Ouyang, Qiaoying Zhu, Jingjun Huang, Linqiang Han, Shuisheng Li, Guifeng Li, Haoran Lin, Yong Zhang
Summary: The Dmrt gene family, including dmrt1, dmrt2, and dmrt3, plays a critical role in sex determination and differentiation in animals. This study demonstrated that dmrt1 is mainly expressed in the testis and is crucial for testis differentiation/development, while dmrt2 is critical in ovary development. The expression patterns and functions of these genes provide important insights into sex differentiation in the mandarin fish.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Jia Xiang Ji, Lin Zhang, Li Li, Kai Lun Wang, Jing Hou, Lan Hao Liu, Bo Li, Bai Dong Zhang, Nan Li, Shan Nan Chen, Pin Nie
Summary: The pIgR gene was cloned in Siniperca chuatsi and found to have a similar protein structure as other fish. The expression of pIgR varied in different organs/tissues and increased significantly during infection with Flavobacterium columnare. pIgR was found to interact with IgT and IgM.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yawei Shen, Huiyang Li, Jinliang Zhao, Shoujie Tang, Yan Zhao, Yanhui Bi, Xiaowu Chen
Summary: The study analyzed the morphological changes and gene expression profiles in the liver, stomach, and intestines of Mandarin fish after artificial diet feeding. Results showed that Mandarin fish have a certain adaptability to artificial diet feeding, allowing for large-scale aquaculture, but multigeneration screening is necessary to obtain strains that are easy to domesticate.
Article
Biochemistry & Molecular Biology
Shiyan Liu, Chong Han, Jingjun Huang, Meihui Li, Jiayu Yang, Guifeng Li, Haoran Lin, Shuisheng Li, Yong Zhang
Summary: In this study, we identified and analyzed the expression pattern of TGF-beta signaling pathway members in mandarin fish during gonadal development. Our results reveal differential expression and participation of TGF-beta signaling pathway members in different stages and sexes of gonadal development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Yueqi Wang, Yingying Shen, Yanyan Wu, Chunsheng Li, Laihao Li, Yongqiang Zhao, Xiao Hu, Ya Wei, Hui Huang
Summary: This study investigated and compared bacterial profiles and flavor compounds in three typical fermented mandarin fish products, revealing clear differences in microbiota among the products. Several bacteria were found to be correlated with the production of important flavor compounds, with three genera identified as the primary microorganisms contributing to more than six characteristic flavor compounds in fermented mandarin fish products.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Fisheries
Haofeng Ouyang, Niuniu Deng, Jiachang Xu, Jingjun Huang, Chong Han, Dingrui Liu, Shiyan Liu, Baohua Yan, Linqiang Han, Shuisheng Li, Guifeng Li, Jin Zhang, Haoran Lin, Yong Zhang
Summary: This study investigated the effects of hyperosmotic stress on intestinal functions, including the intestinal microecosystem, intestinal immune response-related gene expression, and intestinal antioxidant capacity in mandarin fish. The results showed that hyperosmotic stress could alter the composition of the intestinal microbial community, affect immune-related gene expression, and increase intestinal alkaline phosphatase and acid phosphatase activity. These findings provide important insights into the effects of saline-alkali water on the health and immune functions of mandarin fish, as well as the complex relationship between intestinal flora and host disease resistance.
Article
Virology
Jie Zhang, Hong Guo, Fuxian Zhang, Qingxiu Chen, Mingxian Chang, Qin Fang
Article
Fisheries
Xiao Man Wu, Lu Cao, Yi Wei Hu, Ming Xian Chang
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Immunology
Xiao Man Wu, Jie Zhang, Peng Wei Li, Yi Wei Hu, Lu Cao, Songying Ouyang, Yong Hong Bi, Pin Nie, Ming Xian Chang
JOURNAL OF IMMUNOLOGY
(2020)
Review
Fisheries
Ming Xian Chang, Fan Xiong, Xiao Man Wu, Yi Wei Hu
Summary: NLR proteins are immune sensors that recognize pathogen patterns, with different species having varying numbers of NLRs. The zebrafish genome contains almost 400 NLR proteins. These proteins can positively or negatively regulate immune responses and inflammatory signaling cascades.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ming Xian Chang, Fan Xiong
Article
Fisheries
Fan Xiong, Lu Cao, Xiao Man Wu, Ming Xian Chang
Summary: This study demonstrates the conserved antiviral role of gpbar1 and its function in regulating glycerophospholipids metabolism in teleost.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Ming Xian Chang
Summary: This paper summarizes recent advances in understanding of negative regulators of the RLR signaling pathway in fish, with a focus on regulatory mechanisms inhibiting the function of RIG-I, MDA5, and other factors. The study also discusses important directions for future research in this field.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pengwei Li, Mingxian Chang
Summary: Oxidative stress plays a significant role in inflammatory diseases, characterized by the overproduction of reactive oxygen species (ROS). Inflammatory signaling cascades are initiated through the recognition of DAMPs and PAMPs by PRRs, with different PRRs families affecting the production of ROS and/or oxidative stress. Studying the correlation between oxidative stress and PRRs-mediated signaling pathways is crucial for future therapeutic strategies in inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Xiaoman Wu, Fan Xiong, Hong Fang, Jie Zhang, Mingxian Chang
Summary: In this study, the functional correlation between NOD1 and histone H2A variant in response to Streptococcus agalactiae infection was reported. The deficiency of NOD1 promoted S. agalactiae proliferation and decreased larval survival in zebrafish. The results highlight the importance of NOD1 deficiency in immune-related and metabolic pathways regulation, and the correlation between zebrafish NOD1 and histone H2A variant in defense against S. agalactiae infection.
Article
Immunology
Jie Zhang, Xiao Man Wu, Qin Fang, Yong Hong Bi, Pin Nie, Ming Xian Chang
Summary: The study showed that nonstructural proteins NS38 and NS80 of GCRV can evade host antiviral immune response by targeting the RLR signaling pathway, preventing the production of IFN-stimulated genes and facilitating GCRV replication.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biology
Lu Cao, Hong Fang, Dong Yan, Xiao Man Wu, Jie Zhang, Ming Xian Chang
Summary: The adhesion molecule CD44 drives host defense in zebrafish by regulating apoptosis and autophagy. CD44a variants mediate antibacterial responses through different mechanisms, with CD44a_tv1 promoting apoptosis and CD44a_tv2 inhibiting autophagy to fight against E. piscicida infection.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Jie Zhang, Ming Xian Chang
Summary: This study reveals that a grass carp TBK1 isoform named gcTBK1_tv3 plays a role in restraining the replication and infection of grass carp reovirus (GCRV) by promoting the degradation of the nonstructural proteins NS80 and NS38, impairing the formation of viral inclusion bodies.
JOURNAL OF IMMUNOLOGY
(2023)
Article
Fisheries
Si Yao Zheng, Xinbin Shao, Zhitao Qi, Maocang Yan, Min Hui Tao, Xiao Man Wu, Lining Zhang, Jianzhong Ma, An Li, Ming Xian Chang
Summary: This study firstly demonstrates the functional characterization of zebrafish nos2a during bacterial infection, revealing its regulation of ROS and NO as well as the expression of proinflammatory cytokines, antibacterial genes, and oxidant factors. Furthermore, it finds that inhibition of NO can be effective in protecting against E. piscicida infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Review
Fisheries
Peng Wei Li, Jie Zhang, Ming Xian Chang
Summary: AqRVs are a type of virus that can infect various wild or cultured bony fishes, causing serious muscle and viscera haemorrhage syndrome and significant economic losses worldwide. Research on the viral proteins of AqRVs has revealed their roles in RNA transcription, virion assembly, cell attachment, and viral replication. Additionally, the structural and functional studies of AqRVs have provided insights into their replication and immune evasion strategies, leading to the development of vaccines and antiviral agents for GCRV, the most pathogenic AqRV.
REVIEWS IN AQUACULTURE
(2023)
Article
Microbiology
Yun Jie Song, Jie Zhang, Jun Xiao, Hao Feng, Zhen Xu, Pin Nie, Ming Xian Chang
Summary: This study reveals that in the absence of vitamin D, grass carp reovirus (GCRV) utilizes host vitamin D receptors Vdra/Vdrb to increase hmgcr-dependent cholesterol synthesis for promoting the production of viral inclusion bodies (VIBs). The negative regulation of Vdrs during viral infection can be prevented by vitamin D treatment, enhancing resistance against GCRV.
MICROBIOLOGY SPECTRUM
(2023)
