Article
Oncology
Atsushi Takatori, Md Shamim Hossain, Atsushi Ogura, Jesmin Akter, Yohko Nakamura, Akira Nakagawara
Summary: NLRR1 acts as a key regulator of tumor cell proliferation by promoting cell proliferation through its FNIII domain. Monoclonal antibody N1mAb reduces tumor cell proliferation and sensitizes cells to EGFR inhibitor, indicating NLRR1 as a novel regulatory molecule of RTK function.
FRONTIERS IN ONCOLOGY
(2021)
Article
Environmental Sciences
Jin-Ping Wang, Ji-Ping Yan, Rui-Ling Xiao, Rong-Shan Li
Summary: LRRK2 plays a protective role in acute kidney injury by enhancing podocyte autophagy and cell viability.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Virology
Liyan Wang, Fan Yang, Yixin Xiao, Bin Chen, Fumin Liu, Linfang Cheng, Hangping Yao, Nanping Wu, Haibo Wu
Summary: This study obtained three monoclonal antibodies against influenza virus from mice immunized with the HA protein of A/Michigan/45/2015(H1N1), demonstrating strong preventive and therapeutic effects. Two amino acid mutations were discovered, and one of the antibodies still showed protective efficacy after 24 hours of infection.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Shunhui Wei, Julian Behn, Charlene Priscilla Poore, See Wee Low, Bernd Nilius, Hao Fan, Ping Liao
Summary: This study designed a mouse monoclonal antibody called M4M to block human TRPM4 channel. Using peptide microarray, a binding epitope EPGF within the A2 peptide was identified. Structural analysis showed that EPGF is located externally to the channel pore and A1 peptide is close to the EPGF binding epitope. Electrophysiological study revealed that M4M can block human TRPM4, but not rodent TRPM4.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Rui Chen, Yimin Wen, Enbo Yu, Junpeng Yang, Yixiao Liang, Daili Song, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Sanjie Cao, Xiaobo Huang
Summary: We identified two monoclonal antibodies that effectively neutralized PDCoV, with one targeting the S280-288 epitope and the other targeting the S506-513 epitope. The S280-288 epitope induced specific neutralizing antibodies and provided partial protection against disease in piglets. This study provides valuable insights for understanding the pathogenic mechanism of PDCoV, vaccine design, and antiviral drug development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biotechnology & Applied Microbiology
Yu-Chen Cheng, Shih-Chung Chang
Summary: The study prepared two monoclonal antibodies, 7H6C and YC8, with subtype specificity for H5N8 and H5Nx avian influenza viruses, which can be used for specific detection of HA proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bilal Ahmad, Maria Batool, Moon-Suk Kim, Sangdun Choi
Summary: The study investigated the TLR4 antibody-binding epitopes using a computational-driven approach, identifying key residues impacting antibody affinity and TLR4 structural integrity, as well as predicting a novel epitope. This technique enhances understanding of antibody-antigen interactions and facilitates the development of new monoclonal antibodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Summary: Liew et al. present an efficient monoclonal antibody (mAb) production method, successfully developing mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Veterinary Sciences
Yunlei Cao, Dongmei Han, Yujiao Zhang, Kuan Zhang, Nannan Du, Wu Tong, Guoxin Li, Hao Zheng, Changlong Liu, Fei Gao, Guangzhi Tong
Summary: African swine fever (ASF) is a deadly viral disease affecting pigs with no commercial vaccines available. A monoclonal antibody (mAb) against the p54 protein of ASFV was successfully obtained and shown to have potential for detecting ASF vaccine strains. The synthetic epitope peptide used in the ELISA showed high sensitivity and specificity, indicating promise for ASF epidemic monitoring and control.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Houkun Wang, Xueyang Chen, Lilin Zhu, Xiaowei Fang, Keli Gao, Chun Fang, Jing Liu, Yufang Gu, Xiongyan Liang, Yuying Yang
Summary: This study successfully prepared a monoclonal antibody specific for ALV-J and identified a new epitope of ALV-J Gp85. This finding may assist in the development of specific ALV-J detection methods.
Article
Virology
Huan Hu, Rongfei Liu, Qianlin Li, Jin Wang, Qiang Deng, YuYing Lu, Qin Wu, Zeliang Chen, Jiahai Lu
Summary: In this study, ZIKV envelope protein domain III was expressed in E. coli to produce monoclonal antibodies. Ten monoclonal cell lines were identified, with one (mAb 8B6) showing neutralizing activity against ZIKV. Nine other mAbs could also recognize ZIKV, suggesting their potential use for ZIKV diagnosis.
Article
Biochemistry & Molecular Biology
Huiguang Wu, Chen Li, Xian Sun, Yue Cheng, Zhenhai Chen
Summary: This study focused on the expression and production of the membrane (M) protein of porcine deltacoronavirus (PDCoV). The monoclonal antibody (mAb) 24-A6 against the M protein was successfully prepared using hybridoma technology. The mAb 24-A6 showed specificity for PDCoV and can be used in various immunological assays. The study also identified the antigenic epitope of the M protein and revealed an important overlap between a functionally important viral assembly region and a region recognized by the immune system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alessio Borio, Aurora Holgado, Christina Passegger, Herbert Strobl, Rudi Beyaert, Holger Heine, Alla Zamyatina
Summary: The TLR4/MD-2 complex is a crucial receptor in the innate immune system and plays a major role in inflammation, but dysregulation in its signaling can lead to uncontrolled inflammation and disease. Targeting this receptor complex with anti-inflammatory therapies is promising, but the complexity of ligand recognition and species-specificity presents challenges. Researchers have developed glycolipids that mimic lipid A and inhibit TLR4-mediated inflammation, and these glycolipids were evaluated for their activity in human and murine immune cells. A bis-phosphorylated glycolipid was found to have antagonist activity on human TLR4, but acted as a partial agonist on murine TLR4.
Article
Chemistry, Multidisciplinary
Markus Goethel, Martin Listek, Katrin Messerschmidt, Anja Schloer, Anja Hoenow, Katja Hanack
Summary: This study combined bioinformatic analyses and novel immunization techniques to design monoclonal antibodies for the detection of whole microorganisms, successfully targeting E. coli O157:H7.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Francesca Berni, Ermioni Kalfopoulou, Ana M. Gimeno Cardells, Filippo Carboni, Daan van der Es, Felipe Romero-Saavedra, Diana Laverde, Karmela Miklic, Suzana Malic, Tihana L. Rovis, Stipan Jonjic, Sara Ali, Herman S. Overkleeft, Cornelis H. Hokke, Angela van Diepen, Roberto Adamo, Jesus Jimenez-Barbero, Gijsbert A. van der Marel, Johannes Huebner, Jeroen D. C. Codee
Summary: This study developed and employed a monoclonal antibody against a synthetic TA for molecular-level binding analysis using TA microarrays, ELISA, SPR analyses, and STD-NMR spectroscopy. The findings showed that the number and chirality of GroP residues are crucial for interaction, and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
K. Hasegawa, M. Tagawa, K. Takagi, H. Tsukamoto, Y. Tomioka, T. Suzuki, Y. Nishioka, T. Ohrui, M. Numasaki
CANCER GENE THERAPY
(2016)
Article
Medicine, General & Internal
Muneo Numasaki, Hiroki Tsukamoto, Yoshihisa Tomioka, Yasuhiko Nishioka, Takashi Ohrui
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
(2016)
Correction
Biochemistry & Molecular Biology
Hiroki Tsukamoto, Yuki Yamagata, Ippo Ukai, Shino Takeuchi, Misaki Okubo, Yohei Kobayashi, Sao Kozakai, Kanae Kubota, Muneo Numasaki, Yoshitomi Kanemitsu, Yotaro Matsumoto, Yoshihisa Tomioka
Article
Biochemical Research Methods
Yoshitomi Kanemitsu, Kei Asaji, Yotaro Matsumoto, Hiroki Tsukamoto, Daisuke Saigusa, Chikahisa Mukawa, Tatsuki Tachikawa, Takaaki Abe, Yoshihisa Tomioka
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2017)
Article
Urology & Nephrology
Eikan Mishima, Shinji Fukuda, Chikahisa Mukawa, Akinori Yuri, Yoshitomi Kanemitsu, Yotaro Matsumoto, Yasutoshi Akiyama, Noriko N. Fukuda, Hiroki Tsukamoto, Kei Asaji, Hisato Shima, Koichi Kikuchi, Chitose Suzuki, Takehiro Suzuki, Yoshihisa Tomioka, Tomoyoshi Soga, Sadayoshi Ito, Takaaki Abe
KIDNEY INTERNATIONAL
(2017)
Article
Biochemistry & Molecular Biology
Hiroki Tsukamoto, Shino Takeuchi, Kanae Kubota, Yohei Kobayashi, Sao Kozakai, Ippo Ukai, Ayumi Shichiku, Misaki Okubo, Muneo Numasaki, Yoshitomi Kanemitsu, Yotaro Matsumoto, Tomonori Nochi, Kouichi Watanabe, Hisashi Aso, Yoshihisa Tomioka
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Pharmacology & Pharmacy
Yoshitomi Kanemitsu, Hiroki Tsukamoto, Yotaro Matsumoto, Kanako Nozawa-Kumada, Yoshinori Kondo, Takaaki Abe, Yoshihisa Tomioka
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2018)
Article
Immunology
Hiroki Tsukamoto, Sao Kozakai, Yohei Kobayashi, Risako Takanashi, Takuya Aoyagi, Muneo Numasaki, Shoichiro Ohta, Yoshihisa Tomioka
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Koichi Kikuchi, Daisuke Saigusa, Yoshitomi Kanemitsu, Yotaro Matsumoto, Paxton Thanai, Naoto Suzuki, Koki Mise, Hiroaki Yamaguchi, Tomohiro Nakamura, Kei Asaji, Chikahisa Mukawa, Hiroki Tsukamoto, Toshihiro Sato, Yoshitsugu Oikawa, Tomoyuki Iwasaki, Yuji Oe, Tomoya Tsukimi, Noriko N. Fukuda, Hsin-Jung HO, Fumika Nanto-Hara, Jiro Ogura, Ritsumi Saito, Shizuko Nagao, Yusuke Ohsaki, Satoshi Shimada, Takehiro Suzuki, Takafumi Toyohara, Eikan Mishima, Hisato Shima, Yasutoshi Akiyama, Yukako Akiyama, Mariko Ichijo, Tetsuro Matsuhashi, Akihiro Matsuo, Yoshiaki Ogata, Ching-Chin Yang, Chitose Suzuki, Matthew C. Breeggemann, Jurgen Heymann, Miho Shimizu, Susumu Ogawa, Nobuyuki Takahashi, Takashi Suzuki, Yuji Owada, Shigeo Kure, Nariyasu Mano, Tomoyoshi Soga, Takashi Wada, Jeffrey B. Kopp, Shinji Fukuda, Atsushi Hozawa, Masayuki Yamamoto, Sadayoshi Ito, Jun Wada, Yoshihisa Tomioka, Takaaki Abe
NATURE COMMUNICATIONS
(2019)
Article
Immunology
Hiroki Tsukamoto, Kanae Kubota, Ayumi Shichiku, Masamitsu Maekawa, Nariyasu Mano, Hideo Yagita, Shoichiro Ohta, Yoshihisa Tomioka
Article
Biochemical Research Methods
Koshi Nagai, Baasanjav Uranbileg, Zhen Chen, Amane Fujioka, Takahiro Yamazaki, Yotaro Matsumoto, Hiroki Tsukamoto, Hitoshi Ikeda, Yutaka Yatomi, Hitoshi Chiba, Shu-Ping Hui, Toru Nakazawa, Ritsumi Saito, Seizo Koshiba, Junken Aoki, Daisuke Saigusa, Yoshihisa Tomioka
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Multidisciplinary Sciences
Yoshitomi Kanemitsu, Eikan Mishima, Masamitsu Maekawa, Yotaro Matsumoto, Daisuke Saigusa, Hiroaki Yamaguchi, Jiro Ogura, Hiroki Tsukamoto, Yoshihisa Tomioka, Takaaki Abe, Nariyasu Mano
SCIENTIFIC REPORTS
(2019)
Article
Physiology
Eikan Mishima, Shinji Fukuda, Yoshitomi Kanemitsu, Daisuke Saigusa, Chikahisa Mukawa, Kei Asaji, Yotaro Matsumoto, Hiroki Tsukamoto, Tatsuki Tachikawa, Tomoya Tsukimi, Noriko N. Fukuda, Hsin-Jung Ho, Koichi Kikuchi, Chitose Suzuki, Fumika Nanto, Takehiro Suzuki, Sadayoshi Ito, Tomoyoshi Soga, Yoshihisa Tomioka, Takaaki Abe
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2018)
Article
Chemistry, Analytical
Daisuke Jinno, Yoshitomi Kanemitsu, Kazuki Saitoh, Shinnosuke Nankumo, Hiroki Tsukamoto, Yotaro Matsumoto, Takaaki Abe, Yoshihisa Tomioka
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2017)