Article
Environmental Sciences
Xiaoran Wei, Nan Liu, Jian Song, Chao Ren, Xiaowen Tang, Wei Jiang
Summary: SiO2 nanoparticles can affect the fluidity of cell membranes, with a more significant impact at higher temperatures. Components such as phosphatidyl ethanolamine and phosphoinositide can mitigate the membrane gelation caused by the nanoparticles.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Yanyun Lin, Xingjuan Wu, Kaicheng Wang, Sen Shang, Yubin Gong, Hongwei Zhao, Dai Wu, Peng Zhang, Xiaoyun Lu
Summary: This study examined the THz spectrum of several phospholipid components in biological membranes and found different resonant absorption patterns. The absorption peak at approximately 3.0 THz of DSPE and DPPC was confirmed to originate from a collective vibration of their hydrophobic tails. Additionally, irradiation at 3.1 THz significantly enhanced cell membrane fluidity and improved phagocytosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Sourav Mondal, Harshal V. Barkale, Nilanjan Dey
Summary: A pyrenylated fluorogenic probe has been designed to exclusively detect heavy metal pollutants at the bilayer interface. The probe shows no interaction with metal ions in solution but can efficiently detect multiple metal ions in vesicular aggregates. The response of the probe to metal ions depends on the microenvironment of the bilayer membrane, and different responses are observed in different types of membranes. The probe exhibits improved sensitivity when embedded in phospholipid vesicles with a fluid-like liquid crystalline phase.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Biochemistry & Molecular Biology
Naoyuki Tsuchida, Toshiyuki Takagi, Hiroshi Takahashi, Toshitada Yoshihara, Seiji Tobita, Masashi Sonoyama
Summary: A novel amacrocyclic bisphosphatidylcholine lipid was synthesized and compared with a diether phosphatidylcholine, revealing that the novel lipid exhibited higher thermotropic transition temperature and suppressed dye leakage from vesicles in fluid phase, likely due to the single long transmembrane hydrophobic linkage and lipid diffusion.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Biophysics
Teruhiko Baba, Toshiyuki Takagi, Kimio Sumaru, Toshiyuki Kanamori, Takehisa Dewa, Mamoru Nango
Summary: The study found that partially fluorinated phospholipid DF8CCH8PC formed more expanded and stable membranes compared to its non-fluorinated counterpart DH8CCH8PC, with restricted molecular motion. Although the reconstitution efficiencies of LH2 and LH1-RC into DF8CCH8PC membranes were lower, the membrane proteins showed increased thermostability. This suggests that partially fluorinated PCs may be useful materials for constructing lipid-functional membrane protein assemblies for biotechnological applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Multidisciplinary Sciences
Tadashi Nomura, Kohjiro Nagao, Ryo Shirai, Hitoshi Gotoh, Masato Umeda, Katsuhiko Ono
Summary: The ambient temperature significantly affects the developmental timing in animals, and the adaptive molecular mechanisms that respond to variations in temperature remain unclear. This study found that there is a species-specific thermal sensitivity of Notch signaling in the developing amniote brain, and this sensitivity is associated with developmental robustness.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Elizabeth R. Evans, Amir M. Farnoud, Kristin M. O'Brien, Elizabeth L. Crockett
Summary: The study revealed significant differences in membrane properties between icefishes and red-blooded notothenioids, with icefishes exhibiting higher fluidity and earlier breach in membrane integrity at lower temperatures. Leakage sensitivity in icefishes was notably higher between 0 and 5 degrees Celsius compared to red-blooded notothenioids.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ying Wang, Juan Shen, Fanqiang Meng, Zhaoxin Lu, Fengxia Lv, Libang Zhou, Haizhen Zhao
Summary: The study aimed to investigate the antibacterial mechanism of MLGG by studying its effects on cell membranes. The changes in cell membrane properties of B. cereus exposed to different concentrations of MLGG were evaluated. It was found that low concentrations of MLGG prolonged the lag phase of B. cereus cells, while high concentrations resulted in a reduction in bacterial populations. MLGG treatment caused membrane depolarization and an increase in membrane fluidity, with changes observed in fatty acid compositions. Resistance tests indicated the effectiveness of MLGG as a bacteriostatic agent.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alessio Ausili, Illya Yakymenko, Jose A. Teruel, Juan C. Gomez-Fernandez
Summary: Clotrimazole, an azole antifungal drug, was found to affect the transition temperature of phospholipid vesicles and preferentially locate near the C2 and C3 carbons of the fatty acyl chains in the lipid membrane through differential scanning calorimetry and NMR techniques.
Article
Biochemistry & Molecular Biology
Adam Wronski, Izabela Dobrzynska, Szymon Sekowski, Wojciech Luczaj, Ewa Olchowik-Grabarek, Elzbieta Skrzydlewska
Summary: This study aimed to evaluate the effects of phytocannabinoids (CBD and CBG), used singly and in combination, on the composition and function of keratinocyte membranes exposed to UVA radiation. The results showed that the combined use of CBD and CBG partially prevented the increase in phosphatidylinositols, sialic acid, and sphingomyelinase activity induced by UVA radiation in keratinocytes. Simultaneous use of these phytocannabinoids also reduced the formation of lipid rafts and malondialdehyde, preserving the integrity and functionality of keratinocyte membranes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Yue Sun, Songfan Tian, Muhammad Hussain, Shuang Lin, Yue Pan, Xiaodong Li, Lu Liu, Xinyu Lu, Chunmei Li, Youbin Leng, Shilong Jiang
Summary: This study used a lipidomics approach to investigate the differences in lipidome and fatty acid composition among human, bovine, and caprine milk fat globule membrane (MFGM). The results revealed variations in lipid classes and fatty acids between different types of MFGM, providing valuable insights for the design of new infant formula.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Bingen G. Monasterio, Noemi Jimenez-Rojo, Aritz B. Garcia-Arribas, Howard Riezman, Felix M. Goni, Alicia Alonso
Summary: The study demonstrated that cells grown under sphingolipid-restricted conditions have a certain level of survival ability. Enriching with sphingomyelin can restore cell growth rates, and the restriction has significant effects on membrane properties and composition in both plasma membrane and whole cell lipids. Force measurements and mass-spectroscopic analysis revealed that restricting sphingolipid supply can significantly impact the membrane characteristics and composition.
Article
Biochemistry & Molecular Biology
Uxue Ballesteros, Emilio J. Gonzalez-Ramirez, Igor de la Arada, Jesus Sot, Asier Etxaniz, Felix M. Goni, Alicia Alonso, Lidia Ruth Montes
Summary: N-maleimide-derivatized phospholipids, particularly PE-mal, have been investigated for their potential usage in protein anchoring to membranes. This study compared the properties of native PE and PE-mal by using spectroscopic and calorimetric techniques on vesicles containing either PE or PE-mal. The results showed that the presence of PE-mal did not perturb the bilayer's fatty acyl chains and permeability, but did increase the order and decrease the fluidity of the bilayers. Additionally, PE-mal induced conformational changes in the polar heads and increased hydration. Overall, the study suggests that PE-mal could serve as a reasonable substitute for PE in model membranes with reconstituted proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Esther Benayas, Ana Espinosa, M. Teresa Portoles, Virginia Vila-del Sol, M. Puerto Morales, Maria C. Serrano
Summary: In this study, two different superparamagnetic iron oxide multicore nanoparticles (SPIONs) with slightly different physicochemical properties were used to investigate their interaction with primary neural cells. The results showed that the minor differences in surface charge, magnetic properties, and structure of the SPIONs had a significant impact on cell uptake, viability, lipid composition, and membrane fluidity. These findings highlight the importance of considering even minor physicochemical differences in nanomaterials for specific cellular and molecular targeting.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Kristelle Hughes, Thanh Binh Le, Patrick Van Der Smissen, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq, Joelle Quetin-Leclercq
Summary: Leishmaniasis, a neglected tropical disease, still infects thousands of people per year worldwide. Resistance to major treatments has become a healthcare burden, especially in low-income countries. Eugenol, a phenylpropanoid, has shown antileishmanial activity against Leishmania mexicana mexicana (Lmm), but its mechanism of action is still unclear.
Article
Biochemistry & Molecular Biology
Grzegorz Pawlik, Mike F. Renne, Matthijs A. Kol, Anton I. P. M. de Kroon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Editorial Material
Cell Biology
Mike F. Renne, Brooke M. Emerling
JOURNAL OF CELL BIOLOGY
(2020)
Review
Cell Biology
Mike F. Renne, Yoel A. Klug, Pedro Carvalho
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2020)
Letter
Biotechnology & Applied Microbiology
Mike F. Renne, Xue Bao, Anton I. P. M. de Kroon
FEMS YEAST RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Mingjuan Li, M. Ingmar van Raath, Shervin Khakpour, Ahmet Secilir, Bart C. Sliggers, Xuan Huang, Baoyue Ding, Gert Storm, Rene R. van der Hulst, Anton I. P. M. de Kroon, Michal Heger
Article
Biochemistry & Molecular Biology
Xue Bao, Martijn C. Koorengevel, Marian J. A. Groot Koerkamp, Amir Homavar, Amrah Weijn, Stefan Crielaard, Mike F. Renne, Joseph H. Lorent, Willie Jc Geerts, Michal A. Surma, Muriel Mari, Frank C. P. Holstege, Christian Klose, Anton I. P. M. de Kroon
Summary: Phosphatidylcholine (PC) is an essential membrane lipid component in most eukaryotes, but its necessity can be suppressed in yeast through cellular evolvability mechanisms involving chromosomal monosomy or a point mutation in the ACC1 gene. These adaptations lead to rewiring of lipid biosynthesis pathways and decreased activity of Acc1, resulting in altered acyl chain length and regulation of membrane fluidity.
Review
Cell Biology
Mike F. Renne, Hanaa Hariri
Summary: Cells prepare for changes in nutrient availability by storing energy in Lipid Droplets (LDs). Fatty acids released from LDs are trafficked to different cellular compartments for membrane biogenesis or energy production. Inter-organelle contact sites between LDs and organelles like the Endoplasmic Reticulum and mitochondria facilitate FA trafficking and metabolism.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mike F. Renne, Xue Bao, Margriet W. J. Hokken, Adolf S. Bierhuizen, Martin Hermansson, Richard R. Sprenger, Tom A. Ewing, Xiao Ma, Ruud C. Cox, Jos F. Brouwers, Cedric H. De Smet, Christer S. Ejsing, Anton I. P. M. de Kroon
Summary: Our study reveals the importance of ERMES and Vps39p in mediating the transfer of di-unsaturated phospholipids to mitochondria. This supports the concept that lipid flow between membranes is limited by molecular species-dependent lipid efflux and concentration gradients between membranes.
Article
Multidisciplinary Sciences
Yoel A. Klug, Justin C. Deme, Robin A. Corey, Mike F. Renne, Phillip J. Stansfeld, Susan M. Lea, Pedro Carvalho
Summary: The study revealed the mechanism of lipid droplet formation by the coordination of yeast Seipin Sei1 and its partner Ldb16. Sei1 assembles a homooligomeric ring in the ER to concentrate triacylglycerol, promoting Ldb16 stability and lipid droplet assembly.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Cell Biology
Mike F. Renne, Robert Ernst
Summary: The paper discusses two papers, one that discovered and another that structurally defined ERMES, which facilitates the exchange of lipids between the ER and mitochondria.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mike F. Renne, Robert Ernst
Summary: This article discusses the limitations and common misconceptions surrounding membrane responsiveness. By emphasizing the importance of membrane thickness and regulated compressibility in the life cycle of transmembrane proteins, the unfolded protein response (UPR) is proposed as a mechanism for maintaining endoplasmic reticulum (ER) membrane homeostasis.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Cell Biology
Mike F. Renne, Robin A. Corey, Joana Verissimo Ferreira, Phillip J. Stansfeld, Pedro Carvalho
Summary: Renne et al. demonstrate that Seipin plays a role in the formation of lipid droplets containing different types of neutral lipids. The interaction between hydroxyl-residues and neutral lipid carboxyl esters is proposed as a general mechanism for Seipin-mediated lipid droplet formation. This finding provides insights into the cellular processes of lipid homeostasis and the function of Seipin.
JOURNAL OF CELL BIOLOGY
(2022)
