Article
Oncology
Ikko Mito, Hideyuki Takahashi, Reika Kawabata-Iwakawa, Momoka Horikawa, Shota Ida, Hiroe Tada, Toshiyuki Matsuyama, Kiyoshi Misawa, Shigeki Takeda, Kazuaki Chikamatsu
Summary: This study investigates the role of exosome-mediated crosstalk between tumor and stromal cells in HNSCC. It demonstrates that exosomes released from HNSCC tumor cells can convert normal fibroblasts into CAF-like cells and influence their gene expression and function. These findings highlight the importance of exosome-based cell-to-cell communication in creating an inflammatory tumor microenvironment.
Review
Cell Biology
Julia Hohn, Wenbin Tan, Amanda Carver, Hayden Barrett, Wayne Carver
Summary: Changes in the extracellular matrix play a key role in tissue repair processes, especially during fibrosis where excessive matrix alters tissue function. Fibrosis was once thought to be progressive and irreversible, but recent studies have shown it can be stopped and even reversed. Therapeutic approaches targeting fibrosis and matrix-producing cells are being pursued more actively now.
Article
Pharmacology & Pharmacy
Zhiyuan Niu, Lei Wang, Xiaofeng Qin, Zhimin Ye, Bin Xie, Yongbin Hu
Summary: Silicosis is a serious occupational disease characterized by inflammation, fibroblast activation, and extracellular matrix deposition in the lung. Macrophage-derived exosomes have been found to play a role in the development of silicosis by transferring microRNAs (miRNAs). In this study, the researchers identified the up-regulation of miR-7219-3p in both macrophages and their exosomes after silica exposure, as well as in a silicotic mice model. They found that miR-7219-3p promoted fibroblast to myofibroblast trans-differentiation, cell proliferation, and migration. The target gene of miR-7219-3p, Spouty1 (SPRY1), was confirmed to modulate the Ras/ERK/MAPK signaling pathway, and manipulating SPRY1 expression affected fibroblast to myofibroblast trans-differentiation via this pathway. Inhibition of exosomal miR-7219-3p partially suppressed fibroblast to myofibroblast trans-differentiation and silica-induced pulmonary fibrosis. These findings suggest that exosomal miR-7219-3p could be a potential therapeutic target for silicosis.
Article
Nanoscience & Nanotechnology
Kai Chen, Tao Yu, Xin Wang
Summary: Research found that exosome-derived miRNA from diabetic patients slows down wound healing process by suppressing vascular endothelial growth factor A expression.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Shidi Wu, Marion Rietveld, Marieke Hogervorst, Frank de Gruijl, Sjoerd van der Burg, Maarten Vermeer, Remco van Doorn, Marij Welters, Abdoelwaheb El Ghalbzouri
Summary: The study demonstrated that papillary and reticular fibroblasts in the dermis have distinct effects on epithelial/non-epithelial tumors, particularly in promoting EMT and invasion processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Francesca Citron, Ilenia Segatto, Lorena Musco, Ilenia Pellarin, Gian Luca Rampioni Vinciguerra, Giovanni Franchin, Giuseppe Fanetti, Francesco Micciche, Vittorio Giacomarra, Valentina Lupato, Andrea Favero, Isabella Concina, Sanjana Srinivasan, Michele Avanzo, Isabella Castiglioni, Luigi Barzan, Sandro Sulfaro, Gianluigi Petrone, Andrea Viale, Giulio F. Draetta, Andrea Vecchione, Barbara Belletti, Gustavo Baldassarre
Summary: EGFR activation upregulates miR-9 expression, sustaining aggressiveness and resistance to RT+CTX in HNSCC. miR-9 may serve as a valuable biomarker for selecting HNSCC patients who may benefit from RT+CTX therapy.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon Seo
Summary: It is unclear how scar fibroblasts (SFs) affect keratinocytes in hypertrophic scars (HTS) through cell-cell interaction. This study investigated the effects of HTS-derived exosomes on the proliferation and differentiation of normal human keratinocytes (NHKs) and compared them with normal fibroblasts (NFs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Virginia Napolitano, Daniela Russo, Francesco Morra, Francesco Merolla, Silvia Varricchio, Gennaro Ilardi, Rosa Maria Di Crescenzo, Francesco Martino, Massimo Mascolo, Angela Celetti, Luca Tamagnone, Stefania Staibano
Summary: NRP-1, as a co-receptor of EGFR, correlates with poor prognosis and modulates response to cisplatin treatment in HNSCC, suggesting its potential as a therapeutic target for novel approaches in treating this aggressive malignancy characterized by overexpression of EGFR.
Article
Cell Biology
Enxian Shi, Zhengquan Wu, Birnur Sinem Karaoglan, Sabina Schwenk-Zieger, Gisela Kranz, Nilofer Abdul Razak, Christoph A. Reichel, Martin Canis, Philipp Baumeister, Reinhard Zeidler, Olivier Gires
Summary: CD73 is identified as an effector of EGFR-mediated local invasion and a potential therapeutic target and predictive marker for advanced HPV-negative HNSCC. It is correlated with EGFR pathway activity, EMT, and partial EMT in malignant HNSCC cells. CD73 may have clinical implications in the treatment and prediction of HPV-negative advanced HNSCC.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Review
Oncology
Amina Jouida, Cormac McCarthy, Aurelie Fabre, Michael P. Keane
Summary: Exosomes play a crucial role in cell to cell communication by transferring biological material, and have implications in tumor initiation, promotion, and progression. They are emerging as potential biomarkers for early detection, diagnosis, prognosis assessment, and therapeutic response prediction in EGFR-mutated lung cancer.
CANCER AND METASTASIS REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Henrik Schinke, Enxian Shi, Zhongyang Lin, Tanja Quadt, Gisela Kranz, Jiefu Zhou, Hongxia Wang, Julia Hess, Steffen Heuer, Claus Belka, Horst Zitzelsberger, Udo Schumacher, Sandra Genduso, Kristoffer Riecken, Yujing Gao, Zhengquan Wu, Christoph A. Reichel, Christoph Walz, Martin Canis, Kristian Unger, Philipp Baumeister, Min Pan, Olivier Gires
Summary: This study investigated the role of EGFR-induced epithelial-to-mesenchymal transition (EMT) in head and neck squamous cell carcinoma (HNSCC) progression and identified potential prognostic and therapeutic targets. The results suggest that ITGB4 may serve as a potential therapeutic and predictive target in HNSCC patients with strong EGFR-induced EMT.
Review
Oncology
Daoan Cheng, Banglu Wang, Lige Wu, Rui Chen, Weiqing Zhao, Cheng Fang, Mei Ji
Summary: Lung cancer is the primary cause of cancer-related deaths worldwide. The use of EGFR tyrosine kinase inhibitors has improved survival rates for patients with EGFR-mutated non-small cell lung cancer. However, resistance to these inhibitors, like other anticancer drugs, inevitably develops over time. Exosomes, extracellular vesicles carrying non-coding RNAs, have been found to play a role in the development of EGFR-TKIs resistance. This review provides an overview of the current research on exosomal non-coding RNAs mediating EGFR-TKIs resistance in EGFR-mutated NSCLC, and suggests potential applications in monitoring therapy and developing new treatment strategies.
Article
Pharmacology & Pharmacy
Geonhee Han, Hyosuk Kim, Da Eun Kim, Yeonjoo Ahn, Joongsoo Kim, Ye Ji Jang, Kwangmeyung Kim, Yoosoo Yang, Sun Hwa Kim
Summary: This study found that bovine colostrum-derived exosomes have potential beneficial effects on UV-induced aging and damage. They can prevent the generation of reactive oxygen species in keratinocytes, reduce melanin production in melanocytes, and enhance collagen production in fibroblasts.
Review
Oncology
Morena Fasano, Carminia Maria Della Corte, Giuseppe Viscardi, Raimondo Di Liello, Fernando Paragliola, Francesca Sparano, Maria Lucia Iacovino, Anna Castrichino, Francesca Doria, Antonello Sica, Floriana Morgillo, Giuseppe Colella, Giampaolo Tartaro, Salvatore Cappabianca, Domenico Testa, Gaetano Motta, Fortunato Ciardiello
Summary: Head and neck cancers are the seventh most common cancer worldwide, with squamous cell carcinomas being the most frequent histologic subtype. The current standard treatment includes surgery or radiotherapy for early stage diseases, while locally advanced cases may require a more aggressive multi-modal approach. Anti-EGFR targeted therapy cetuximab is currently the only approved targeted therapy option, with the recent development of immune-checkpoint inhibitors providing new treatment options.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2021)
Article
Oncology
Amina Jouida, Marissa O'Callaghan, Cormac Mc Carthy, Aurelie Fabre, Parthiban Nadarajan, Michael P. Keane
Summary: Exosomes, as extracellular vesicles, have gained attention as potential sources of information on tumor detection and regulation of tumor progression and metastasis. This study found that exosomes from NSCLC patients with EGFR mutations could induce invasion by promoting hybrid EMT, highlighting their role in metastasis formation and establishment of the pre-metastatic niche.
Review
Cell Biology
David K. Dansu, Sami Sauma, Patrizia Casaccia
Summary: In the past decade, there has been a significant revision of the traditional concept of the role and function of glial cells, now recognizing them as metabolic exchangers with neurons and responders to changes in the environment. Oligodendrocyte lineage cells play key roles in brain development and normal brain function in both neonatal and adult stages. They act as environmental biosensors, adapting to chemical and physical stimuli over time by modifying their epigenome and transcriptome to respond to new challenges.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
David W. Andrews, Kevin D. Judy, Charles B. Scott, Samantha Garcia, Larry A. Harshyne, Lawrence Kenyon, Kiran Talekar, Adam Flanders, Kofi-Buaku Atsina, Lyndon Kim, Nina Martinez, Wenyin Shi, Maria Werner-Wasik, Haisong Liu, Mikhail Prosniak, Mark Curtis, Rhonda Kean, Donald Y. Ye, Emily Bongiorno, Sami Sauma, Mark A. Exley, Kara Pigott, D. Craig Hooper
Summary: IGV-001 demonstrated good tolerability in newly diagnosed glioblastoma patients, with a significantly improved median progression-free survival (PFS) compared to standard of care (SOC). In patients eligible for Stupp criteria, IGV-001 showed positive outcomes in terms of PFS and overall survival, suggesting a potential immune-mediated mechanism.
CLINICAL CANCER RESEARCH
(2021)
Article
Immunology
Michael Prosniak, Larry A. Harshyne, Jonathan Gorky, Mark T. Curtis, Lawrence C. Kenyon, James S. Schwaber, Aurore Lebrun, Rhonda B. Kean, David W. Andrews, D. Craig Hooper
Summary: Patients with grade III anaplastic astrocytomas (AA) can be categorized into distinct immune bias profiles based on the presence or absence of mutations in isocitrate dehydrogenase (IDH). Two different immune biases were identified in patients with different IDH mutational status, with type 2 bias more common in IDH wild-type AA and type 1 bias more common in patients with IDH1R132H mutation. Additionally, the patient's immune profile was found to be more closely associated with tumor vascular enhancement on imaging rather than IDH mutational status.
JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Oncology
J. M. Curry, A. Alnemri, S. Sussman, L. Harshyne, A. Linnenbach, R. Stapp, A. South, U. Nwagu, B. Swendseid, M. Tuluc, S. Gargano, D. Cognetti, V. Bar-Ad, A. Luginbuhl, R. Axelrod, D. Whitaker-Menezes, M. G. Mahoney, A. Argiris, U. Martinez-Outschoorn, J. M. Johnson
ANNALS OF ONCOLOGY
(2021)
Article
Oncology
Adam J. Luginbuhl, Jennifer M. Johnson, Larry A. Harshyne, Alban J. Linnenbach, Sanket K. Shukla, Angela Alnemri, Gaurav Kumar, David M. Cognetti, Joseph M. Curry, Nikita Kotlov, Zoya Antysheva, Sandrine Degryse, Kyle Mannion, Michael K. Gibson, James Netterville, Brandee Brown, Rita Axelrod, Ralph Zinner, Madalina Tuluc, Stacey Gargano, Benjamin E. Leiby, Ayako Shimada, My G. Mahoney, Ubaldo Martinez-Outschoorn, Ulrich Rodeck, Young J. Kim, Andrew P. South, Athanassios Argiris
Summary: The addition of phosphodiesterase-5 inhibitor tadalafil to the PD-1 inhibitor nivolumab is safe and enhances immune-mediated antitumor responses in previously untreated squamous cell carcinoma of the head and neck. The combination therapy results in a pathologic treatment response of at least 20% in over 50% of the patients after 4 weeks of treatment. Pretreatment specimens show HPV status-dependent signatures that predict response to immunotherapy, while posttreatment specimens demonstrate an enhanced immune microenvironment with the addition of tadalafil.
CLINICAL CANCER RESEARCH
(2022)
Meeting Abstract
Oncology
I. Vathiotis, J. M. Johnson, L. Harshyne, A. Luginbuhl, J. M. Curry, D. Cognetti, R. Axelrod, V. Bar-Ad, A. Argiris
ANNALS OF ONCOLOGY
(2022)
Meeting Abstract
Oncology
J. Riordan, A. Linnenbach, A. Alnemri, R. Philips, S. Awosanya, S. Sussman, A. Luginbuhl, D. Cognetti, L. Harshyne, M. Mahoney, A. South, D. Whitaker-Menezes, M. Tuluc, A. Argiris, U. Martinez-Outschoorn, J. M. Johnson, J. M. Curry
ANNALS OF ONCOLOGY
(2022)
Meeting Abstract
Oncology
L. Harshyne, A. Linnenbach, M. Mahoney, A. South, U. Martinez-Outschoorn, J. M. Curry, D. Cognetti, J. M. Johnson, M. Tuluc, A. Argiris, A. Luginbuhl
ANNALS OF ONCOLOGY
(2022)
Meeting Abstract
Oncology
A. Luginbuhl, E. Scott, L. Harshyne, E. Flerova, M. Tuluc, S. Gargano, J. M. Curry, D. Cognetti, A. Argiris, J. M. Johnson
ANNALS OF ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Joseph Curry, Angela Alnemri, Ramez Philips, Michele Fiorella, Sarah Sussman, Robert Stapp, Charalambos Solomides, Larry Harshyne, Andrew South, Adam Luginbuhl, Madalina Tuluc, Ubaldo Martinez-Outschoorn, Athanassios Argiris, Alban Linnenbach, Jennifer Johnson
Summary: This study analyzed the cellular density and intercellular distances of CD8+ and FoxP3+ T-cells in head and neck squamous cell carcinoma samples. The results showed that the distribution of CD8+ and FoxP3+ cells was associated with treatment outcomes and pathological treatment response. Increased CD8+-FoxP3+ intercellular distances were closely related to pathological treatment response.
Article
Immunology
Christopher Herbst, Larry A. Harshyne, Botond Z. Igyarto
Summary: Dendritic cells can actively acquire antigens from neighboring cells and assess the health of the donor cells through cell-cell interactions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Brianna L. L. Hill, Alyssa N. N. Calder, Joseph P. P. Flemming, Yiyang Guo, Sydney L. L. Gilmore, Melissa A. A. Trofa, Sean K. K. Daniels, Torbjoern N. N. Nielsen, Laura K. K. Gleason, Zoya Antysheva, Ksenia Demina, Nikita Kotlov, Christopher J. H. Davitt, David M. M. Cognetti, George C. C. Prendergast, Adam E. E. Snook, Jennifer M. M. Johnson, Gaurav Kumar, Alban J. J. Linnenbach, Ubaldo Martinez-Outschoorn, Andrew P. P. South, Joseph M. M. Curry, Larry A. A. Harshyne, Adam J. J. Luginbuhl, My G. Mahoney
Summary: Therapy using anti-PD-1 immune checkpoint inhibitors has revolutionized cancer treatment, but only a fraction of patients respond. The study identified Dsg2, miR-146a, and IL-8 as potential biomarkers for ICI response in HPVpos HNSCC patients, suggesting that targeting the Dsg2/miR-146a/IL-8 signaling axis could improve ICI responsiveness.
MOLECULAR CARCINOGENESIS
(2023)
Meeting Abstract
Oncology
Adam Luginbuhl, Jennifer Maria Johnson, Elizabeth R. Scott, Larry Harshyne, Elizaveta Flerova Flerova, Madalina Tuluc, Stacey Gargano, Benjamin Leiby, Matthew O. Old, Nolan Seim, Joseph M. Curry, David M. Cognetti, Athanassios Argiris
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Meeting Abstract
Oncology
Nikita Kotlov, Zoya Antysheva, Dina Antonova, Naira Samarina, Sandrine Degryse, Alban Linnenbach, Sanket Shukla, Larry Harshyne, Andrew P. South, Jennifer M. Johnson, Young Kim, Alexander Bagaev, Nathan H. Fowler, Adam Luginbuhl
