Article
Biochemistry & Molecular Biology
Min Li, Yun Zhang, Jun Zhou, Hongmei Liu
Summary: SELENOF deficiency in young mice leads to glucose metabolism disorders, which improve with age. The knockout of SELENOF also causes oxidative stress and disrupts redox homeostasis in young mice.
Article
Ophthalmology
Renita Maria Martis, Bo Li, Paul James Donaldson, Julie Ching-Hsia Lim
Summary: The study found that loss of xCT resulted in depletion of glutathione in the epithelium and an oxidative shift in the cysteine/cystine ratio of the aqueous humor, accelerating the development of an anterior cataract in knockout mice.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Takayuki Kobayashi, Hiroyuki Uchino, Eskil Elmer, Yukihiko Ogihara, Hidetoshi Fujita, Shusuke Sekine, Yusuke Ishida, Iwao Saiki, Shoichiro Shibata, Aya Kawachi
Summary: In this study, a SAE model was established using CypD KO mice, and metabolomic analysis showed that the GSH levels were maintained in the KO animals following CLP. This suggests that CypD may play a role in the pathogenesis of SAE by inhibiting MPTP induction and protecting mitochondrial and cellular function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chang Ho Yoon, Jin Suk Ryu, Jung Hwa Ko, Yeon Kyung Kim, Jang-Hee Oh, Jin Ho Chung, Joo Youn Oh
Summary: The study revealed the impact of Fut1 gene knockout on meibomian glands, showing that Fut1 KO mice had smaller total and acinar areas in comparison to WT mice, decreased meibocyte differentiation, lipid synthesis, and increased inflammation and oxidative stress levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Paul A. Hyslop, Leonard N. Boggs, Michael O. Chaney
Summary: H2O2 oxidation leads to rapid S-glutathionylation of catalytic cysteine residues (C-c(SH)) of GAPDH. Restoration of enzyme activity is achieved through thiol/disulfide exchange, forming glutathione disulfide (G(SS)G) and active enzyme. Molecular dynamic simulations reveal that S-glutathionylation and G(SS)G perturb the subunit secondary structure, particularly within the S-loop region. These findings provide insights into the elevated S-glutathionylated GAPDH in neurodegenerative diseases and suggest potential therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Lingte Huang, Zeen Yang, Jiankun Yuan, Siying Zuo, Zengqi Li, Kelei Yang, Shang Wang, Jia Li, Liyun Zhu, Yongjun Zhang
Summary: Pumpkin polysaccharides can improve abnormal glucose metabolism caused by oxidative stress, significantly reduce blood glucose and lipid levels. In addition, pumpkin polysaccharides can increase the activity of antioxidant enzymes and protect against oxidative stress damage in the liver.
Article
Biochemistry & Molecular Biology
Kang Han, Xiaofang Jin, Xin Guo, Guoli Cao, Siyu Tian, Yiming Song, Yuanyuan Zuo, Peng Yu, Guofen Gao, Yan-Zhong Chang
Summary: Nrf2 plays a crucial role in regulating brain iron metabolism, with Nrf2 KO improving motor function in aged mice while reducing oxidative stress and neuronal apoptosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Margaret E. Maes, Gloria Colombo, Florianne E. Schoot Uiterkamp, Felix Sternberg, Alessandro Venturino, Elena E. Pohl, Sandra Siegert
Summary: Mitochondrial networks in microglia show different adaptations in vitro and in vivo, with increased fragmentation, content, and perinuclear localization observed in vivo. Knockout of UCP2 in microglia after injury induces cellular stress and results in mitochondrial hyperfusion in male microglia, which is absent in females due to circulating estrogens.
Article
Biochemistry & Molecular Biology
Yang Huang, Zi Ye, Yujing Yin, Tianju Ma, Qi Zhang, Kun Shang, Wenqian Chen, Zhaohui Li
Summary: The loss of HO-1 enzymatic activity induces early-onset nuclear cataracts by activating oxidative stress and ER stress.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Food Science & Technology
Yueting Ge, Bowen Li, Yuhui Yang, Chuanxing Feng, Xue Tang, Yonghui Shi, Guowei Le, Jin Sun
Summary: Consumption of pork with high levels of oxidative injury can lead to disorders of glucose metabolism in mice, characterized by hyperglycemia, hypoinsulinemia, impaired glucose tolerance, and impaired pancreatic insulin secretion. Protein oxidation plays a key role in this abnormal glucose metabolism induced by high oxidative injury pork intake.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Chemistry, Analytical
Paula Menero-Valdes, Michail I. Chronakis, Beatriz Fernandez, Jr C. Derrick Quarles, Hector Gonzalez-Iglesias, Bjoern Meermann, Rosario Pereiro
Summary: An automated and straightforward detection and data treatment strategy using sc-ICP-ToF-MS was proposed to determine the protein relative concentration in individual human cells. Metal nanocluster-labeled specific antibodies and ruthenium red staining were employed to evaluate the number of cell events and cell volume. The proposed approach allows for the determination of both the mass and relative concentration of target proteins in each individual cell.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Yifang Cao, Li Li, Yan Zhang, Fengsong Liu, Xinglong Xiao, Xiaofeng Li, Yigang Yu
Summary: The study shows that sdiA affects biofilm formation in C. sakazakii by regulating gene expression related to bacterial motility and biofilm characteristics. Biofilm formation increased under low osmotic stress conditions and decreased under high osmotic stress conditions.
FOOD RESEARCH INTERNATIONAL
(2022)
Review
Cell Biology
Manuel Ruiss, Oliver Findl, Martin Kronschlaeger
Summary: Cataract is a leading cause of blindness globally, and surgery is currently the only treatment option. However, complications and limited access to ophthalmic care may arise. With a growing and aging population, there will be an increase in cataract prevalence, presenting a challenge to society. As oxidative stress is a major pathological process in cataract formation, antioxidant supplementation may be a strategy to delay or prevent its progression. Caffeine, a widely consumed antioxidant, shows potential for preventing cataract.
AGEING RESEARCH REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Stephanie N. Alu, Evan A. Los, George A. Ford, William L. Stone
Summary: Considerable evidence supports the role of oxidative stress in adult type 2 diabetes (T2D), and this will also affect more and more children. Understanding the factors contributing to oxidative stress and T2D risk may help develop optimal early intervention strategies. The combination of systems medicine and redoxomics holds promise in optimizing future healthcare strategies for T2D patients.
Article
Chemistry, Medicinal
Qianxia Lin, Yueping Guo, Jie Li, Shuqi He, Yan Chen, Huoxi Jin
Summary: In this study, peptides with a molecular weight of less than 1 kD (HNCP) derived from Harpadon nehereus bone collagen were found to attenuate oxidative stress, reduce blood glucose, and improve glycolipid metabolism in streptozocin-induced type 1 diabetes mice.
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)