Article
Multidisciplinary Sciences
Nikhil T. Awatade, Andrew T. Reid, Kristy S. Nichol, Kurtis F. Budden, Punnam Chander Veerati, Prabuddha S. Pathinayake, Christopher L. Grainge, Philip M. Hansbro, Peter A. B. Wark
Summary: This study compared the morphology and functional responses of primary bronchial epithelial cells differentiated using two commonly used culture media, PneumaCult and bronchial epithelial growth medium. The results showed distinct structural and functional differences between the two media.
SCIENTIFIC REPORTS
(2023)
Article
Ophthalmology
Guoliang Wang, Lina Xu, Ruize Shi, Yingyue Ye, Baihui Zeng, Xiuqin Yang, Zeyu Liu, Zhen Liu, Shurong Wang, Yuhua Xue, Cheng Li
Summary: In this study, a stable organotypic culture model of Meibomian glands (MGs) was established, and the risk factors of MG dysfunction (MGD) were explored using this model. The model serves as a valuable tool for investigating the pathophysiological mechanisms and screening drugs for MG-related diseases.
Article
Cell Biology
Laura von Schledorn, David Puertollano Martin, Nicole Cleve, Janina Zoellner, Doris Roth, Ben Ole Staar, Jan Hegermann, Felix C. Ringshausen, Janna Nawroth, Ulrich Martin, Ruth Olmer
Summary: Primary ciliary dyskinesia (PCD) is a rare genetic disorder that affects the function of motile cilia in the respiratory tract, leading to chronic inflammation and lung damage. Current treatment options for PCD are only symptomatic, highlighting the need for curative therapies. In this study, researchers developed an in vitro model using human induced pluripotent stem cells (hiPSC) to recapitulate the diseased phenotype of PCD at the molecular, structural, and functional levels.
Article
Cell Biology
Jelmer Legebeke, Katie L. Horton, Claire L. Jackson, Janice Coles, Amanda Harris, Htoo A. Wai, John W. Holloway, Gabrielle Wheway, Diana Baralle, Jane S. Lucas
Summary: In this study, the differentiation and recapitulation of pseudostratified epithelium in air-liquid interface (ALI) cell culture were evaluated, showing its usefulness in researching respiratory health and disease. The transcriptomic changes of ALI-culture were compared to ex vivo nasal brushing samples, highlighting the need for co-cultures with immune cell representation to better reflect the physiological state. The study also revealed the timing of ALI-culture harvest for research studies needs to be carefully considered, as different time-points correspond to different gene expressions and cell functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Environmental Sciences
Henri Hakkarainen, Anssi Jarvinen, Teemu Lepisto, Laura Salo, Niina Kuittinen, Elmeri Laakkonen, Mo Yang, Maria -Viola Martikainen, Sanna Saarikoski, Minna Aurela, Luis Barreira, Kimmo Teinila, Mika Ihalainen, Paivi Aakko-Saksa, Hilkka Timonen, Topi Ronkko, Pasi Jalava
Summary: The differences in traffic fuels impact exhaust emissions and their toxicity, with the aromatic content of diesel fuel playing a significant role. Ultra-fine particles are important components of engine emissions and have health effects. It is crucial to study the toxicity of these particles and how different fuel options can mitigate emissions and toxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Chemical
Rui-Wen He, Hedwig M. Braakhuis, Rob J. Vandebriel, Yvonne C. M. Staal, Eric R. Gremmer, Paul H. B. Fokkens, Claudia Kemp, Jolanda Vermeulen, Remco H. S. Westerink, Flemming R. Cassee
Summary: Inhalation exposure to aerosol contaminants can lead to respiratory health problems. To mimic long-term exposure for toxicity testing, lung epithelial cells must be maintained under ALI conditions and co-cultured with macrophages. Calu-3 cells maintain monolayer structure and tight junctions, making them ideal for co-culturing with macrophages for increased sensitivity in responses to aerosol exposure.
JOURNAL OF AEROSOL SCIENCE
(2021)
Review
Virology
Natalie Heinen, Mara Kloehn, Eike Steinmann, Stephanie Pfaender
Summary: SARS-CoV-2 has spread rapidly, leading scientists and pharmaceutical companies worldwide to urgently develop new treatment options and vaccines. Preclinical cell culture techniques are increasingly being utilized to study the pathogenesis and drug efficacy of SARS-CoV-2. These cell culture systems are helping to address the challenges posed by the current COVID-19 pandemic.
Article
Biotechnology & Applied Microbiology
Cristina Garcia-Mouton, Alberto Hidalgo, Raquel Arroyo, Mercedes Echaide, Antonio Cruz, Jesus Perez-Gil
Summary: This research focuses on delivering full-length recombinant human surfactant protein SP-D using pulmonary surfactant at the respiratory air-liquid interface. Results demonstrate that pulmonary surfactant can transport rhSP-D long distances over air-liquid interfaces, either separately or in combination with PS, opening new possibilities for enhancing clinical surfactants and surfactant replacement therapy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Christie M. Sayes, Madhuri Singal
Summary: The study compared two different aerosol exposure apparatuses: a settling chamber and a gentle impactor. The gentle impactor showed higher deposition efficiency and shorter exposure duration, indicating greater potential as a high-throughput exposure tool.
JOURNAL OF AEROSOL SCIENCE
(2021)
Review
Pharmacology & Pharmacy
Pavel Rossner, Tereza Cervena, Michal Vojtisek-Lom
Summary: Outdoor air pollution, especially from vehicle engine emissions, has a significant negative impact on human health. Therefore, the development of reliable test systems to assess the health effects of engine emissions exposure is crucial.
Article
Biochemistry & Molecular Biology
Luis Soriano, Tehreem Khalid, Fergal J. O'Brien, Cian O'Leary, Sally-Ann Cryan
Summary: Translation of novel inhalable therapies for respiratory diseases is hindered by the lack of in vitro cell models reflecting native tissue complexity. Developing physiologically-representative tracheobronchial tissue analogues can improve new treatments translation by accurately reflecting in vivo respiratory responses. The study utilized advanced tissue-engineered collagen hyaluronic acid bilayered scaffolds (CHyA-B) to evaluate bacterial and drug-induced toxicity and inflammation, finding higher inflammatory responses in 3D epithelial cultures compared to 2D.
Article
Toxicology
Rui Xiong, Leihong Wu, Yue Wu, Levan Muskhelishvili, Qiangen Wu, Ying Chen, Tao Chen, Matthew Bryant, Hans Rosenfeldt, Sheila M. Healy, Xuefei Cao
Summary: Exposure to cigarette smoke has been shown to impair mucociliary clearance and is associated with chronic obstructive pulmonary diseases. In this study, miRNAs associated with impaired mucociliary clearance in an in vitro airway tissue model exposed to cigarette smoke were identified. The downregulation of miR-449a by cigarette smoke may interfere with ciliogenesis and derepress cell-cycle proteins, potentially serving as an early biomarker for cigarette smoke exposure and harm.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Environmental Sciences
Paul D. Robinson, Farhad Salimi, Christine T. Cowiee, Samuel Clifford, Gregory G. King, Cindy Thamrin, Kate Hardaker, Mandana Mazaherie, Lidia Morawska, Brett G. Toelle, Guy B. Marks
Summary: This study observed a modest detrimental effect of ultrafine particles on peripheral airway function among atopic students, potentially leading to respiratory stiffness. Further studies are needed to explore the pathophysiological mechanisms underlying increased respiratory stiffness, and whether this effect persists into adolescence and adulthood.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Daniele Cei, Ali Doryab, Anke-Gabriele Lenz, Andreas Schroeppel, Paula Mayer, Gerald Burgstaller, Roberta Nossa, Arti Ahluwalia, Otmar Schmid
Summary: The study presents an innovative bioreactor, MALI, which mimics the mechanical motion of lung tissue during breathing to study aerosol deposition on cells. By cycling pressure, the membrane can simulate periodic mechanical strain and maintain cell viability while achieving high deposition efficiency.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Food Science & Technology
T. Ruth, J. Daniel, A. Koening, R. Trittler, M. Garcia-Kaeufer
Summary: The solvent carrier liquid in electronic cigarettes, consisting of propylene glycol and vegetable glycerine, can pose potential lung hazards when heated. Thermochemical transformation of the solvent leads to the formation of toxic substances that may disrupt lung homeostasis.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Christophe Mas, Bernadett Boda, Mireille CaulFuty, Song Huang, Ludovic Wiszniewski, Samuel Constant
JOURNAL OF BIOTECHNOLOGY
(2015)
Review
Neurosciences
Bernadett Boda, Aline Dubos, Dominique Muller
CURRENT OPINION IN NEUROBIOLOGY
(2010)
Article
Neurosciences
Bernadett Boda, Pablo Mendez, Benjamin Boury-Jamot, Fulvio Magara, Dominique Muller
EUROPEAN JOURNAL OF NEUROSCIENCE
(2014)
Article
Neurosciences
Bernadett Boda, Lorena Jourdain, Dominique Muller
Article
Cell Biology
Irina Nikonenko, Bernadett Boda, Sylvain Steen, Graham Knott, Egbert Welker, Dominique Muller
JOURNAL OF CELL BIOLOGY
(2008)
Article
Biochemistry & Molecular Biology
Joao Miguel Cordeiro, Bernadett Boda, Paula P. Goncalves, Yves Dunant
JOURNAL OF NEUROCHEMISTRY
(2013)
Article
Neurosciences
Aline Dubos, Gaelle Combeau, Yann Bernardinelli, Jean-Vianney Barnier, Oliver Hartley, Hubert Gaertner, Bernadett Boda, Dominique Muller
JOURNAL OF NEUROSCIENCE
(2012)
Article
Biochemistry & Molecular Biology
A-M Lepagnol-Bestel, G. Maussion, B. Boda, A. Cardona, Y. Iwayama, A-L Delezoide, J-M Moalic, D. Muller, B. Dean, T. Yoshikawa, P. Gorwood, J. D. Buxbaum, N. Ramoz, M. Simonneau
MOLECULAR PSYCHIATRY
(2008)
Article
Medicine, Research & Experimental
Hulda R. Jonsdottir, Denise Siegrist, Thomas Julien, Blandine Padey, Mendy Bouveret, Olivier Terrier, Andres Pizzorno, Song Huang, Kirandeep Samby, Timothy N. C. Wells, Bernadett Boda, Manuel Rosa-Calatrava, Olivier B. Engler, Samuel Constant
Summary: The SARS-CoV-2 virus has caused a global pandemic with significant economic and societal impact. While vaccines are available, there are disparities in access worldwide. Development of effective antiviral treatments is crucial due to the emergence of new variants and short immunity duration. Researchers evaluated the antiviral activity of single compounds and combinations using a standardized in vitro model. They found that selected drug combinations containing molnupiravir showed enhanced antiviral activity compared to single compound treatments, suggesting their potential as treatment strategies for COVID-19.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Medicine, Research & Experimental
Christophe Mas, Bernadett Boda, Mireille Caul Futy, Song Huang, Ludovic Wisniewski, Samuel Constant
ATLA-ALTERNATIVES TO LABORATORY ANIMALS
(2016)
Article
Biochemistry & Molecular Biology
Patricia Kreis, Emmanuel Thévenot, Véronique Rousseau, Bernadett Boda, Dominique Muller, Jean-Vianney Barnier
JOURNAL OF BIOLOGICAL CHEMISTRY
(2007)
Article
Cell Biology
Roxanne Node-Langlois, Dominique Muller, Bernadett Boda
JOURNAL OF CELL SCIENCE
(2006)
Article
Neurosciences
Bernadett Boda, Irina Nikonenko, Stefano Alberi, Dominique Muller
MOLECULAR NEUROBIOLOGY
(2006)
Article
Anatomy & Morphology
I Nikonenko, B Boda, S Alberi, D Muller
MICROSCOPY RESEARCH AND TECHNIQUE
(2005)