期刊
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
卷 2017, 期 3, 页码 476-483出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.201601280
关键词
Radicals; Nitroxides; Fluorescent probes; Redox chemistry; BODIPYs
资金
- Australian Research Council Centre of Excellence for Free Radical Chemistry and Biotechnology [CE 0561607]
- Australian Research Council Future Fellowships [FT140100746, FT140100986]
- Queensland University of Technology
- Science and Engineering Faculty
- Australian Research Council [FT140100986] Funding Source: Australian Research Council
BODIPY is a highly versatile fluorophore for biological imaging with a tunable fluorescence emission (500-800 nm) that overlaps the optically transparent window for tissue ( 6001300 nm). Herein, we describe the synthesis of optically distinct BODIPY-based profluorescent probes bearing meso- and beta-substituted isoindoline nitroxides and their corresponding methoxyamine derivatives. These profluorescent nitroxide probes possess strongly suppressed fluorescence, which can be revealed upon reduction or reaction with other radicals. Examination of the pentafluorophenylhydrazine reduction of the pre-pared probes by using tandem electron paramagnetic resonance (EPR) and fluorescence spectroscopy demonstrated that the asymmetric bis-beta-substituted probe 9 (lambda(em) = 603 nm) was reduced the fastest; however, the greatest difference in fluorescence emission between the nitroxide and its reduced hydroxylamine analogue was observed for probe 7 (lambda(em) = 570 nm). The significant difference in fluorescence output between the nitroxides and their corresponding diamagnetic derivatives makes these probes ideal tools for imaging reactive oxygen species in biological systems.
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