Article
Polymer Science
Fernando Elias Guckert, Claudia Sayer, Debora de Oliveira, Pedro H. Hermes de Araujo, Bruno Francisco Oechsler
Summary: This work investigates the lipase-catalyzed bulk polycondensation of diethyl succinate and 1,4-butanediol to produce polybutylene succinate (PBS). The study evaluates the molar mass distribution of PBS and the recovery of the biocatalyst for reuse cycles. The results show promising enzymatic polycondensation reactions with good catalytic activity.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Chemistry, Applied
Sara Arana-Pena, Nathalia S. Rios, Diego Carballares, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
Summary: CALB and CRL showed similar properties under different immobilization conditions, while RML displayed significant changes. The effects of immobilization conditions on substrate activity and stability vary greatly, requiring careful selection based on specific circumstances.
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sara Arana-Pena, Diego Carballares, Roberto Morellon-Sterling, Javier Rocha-Martin, Roberto Fernandez-Lafuente
Summary: This study achieved the coimmobilization of lipases from Rhizomucor miehei and Candida antarctica, successfully immobilizing both enzymes even when one of them cannot be immobilized initially. A special strategy was developed to desorb inactivated enzyme and re-immobilize a fresh sample, allowing for enzyme reuse.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nanoscience & Nanotechnology
Jihang Zhang, Zhaoxin Wang, Wei Zhuang, Hesamoddin Rabiee, Chenjie Zhu, Jiawei Deng, Lei Ge, Hanjie Ying
Summary: This study investigates the use of graphene-based materials as immobilized enzymes in the field of biocatalysis. The researchers designed a nanobiological interface between graphene oxide (GO) and lipase TL, which could be tuned through molecular dynamics simulations and chemical modulation. The reduced graphene oxide (rGO) showed improved catalytic activity compared to GO, and 4 hours of reduction with ascorbic acid yielded the highest enzyme activity. The study also explored the drawbacks of hydrophobic nanomaterials on enzyme production and suggested the potential of hydrophilic nanomaterials in enhancing enzyme activity.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Shang Wang, Yan Xu, Xiao-Wei Yu
Summary: The study revealed the crucial role of Phe113 in the interfacial activation of Rhizopus chinensis lipase, showing that it plays a dynamic flipping role in activation. Mutation experiments confirmed the function of Phe113, with the fast flipping rate, high open state proportion, and exposure of the catalytic triad identified as the main reasons for lipase activation. The mutual corroboration of simulations and site-directed mutagenesis results highlighted the critical role of Phe113 in lipase activation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Deyaa Abol-Fotouh, Ola E. A. AlHagar, Mohamed A. Hassan
Summary: This study aimed to produce a highly compatible thermoalkaliphilic lipase using new thermophilic bacterial strains from fish wastes. The optimized fermentation parameters significantly increased the productivity of the TA lipase, which demonstrated outstanding activity and stability in extreme conditions. The purified enzyme showed high specific activity and fold purification, with potential for detergent formulations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Polymer Science
Fernando Elias Guckert, Karina Cesca, Claudia Sayer, Debora de Oliveira, Pedro H. Hermes de Araujo, Bruno Francisco Oechsler
Summary: This study investigated the enzymatic polycondensation of polybutylene succinate (PBS) in solution for biomedical applications. The results showed that reactions containing 5% diphenyl ether as solvent at 80°C and 90°C had the most advantageous synthesis results, with average molar masses between 2,500 and 3,350 g·mol-1. However, it was observed that diphenyl ether affected the stability of the immobilized biocatalyst, indicating the need for further research on using green solvents that maintain the stability of the catalyst.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Energy & Fuels
Fareeha Nadeem, Tahir Mehmood, Zahid Anwar, Shagufta Saeed, Muhammad Bilal, Bisma Meer
Summary: This study demonstrated the maximum production of lipase via solid-state fermentation using lignocellulosic biomass. The optimized conditions led to a high yield of lipase, which was successfully immobilized on chitosan and showed potential for applications in the detergent industry.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Chemistry, Physical
Sanghamitra Sengupta, Rahul Gera, Colin Egan, Uriel N. Morzan, Jan Versluis, Ali Hassanali, Huib J. Bakker
Summary: The interfacial vibrational footprints of SDS and C12E6 binary mixture were investigated using HDVSFG. The presence of C12E6 enhances the effect of SDS on the orientation of interfacial water molecules, contrary to the predictions of the traditional Langmuir adsorption model. Molecular dynamics simulations also support this observation.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Dane Sands, Andrew Davis, Scott Banfield, Ian R. Pottie, Sultan Darvesh
Summary: Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes with various physiological functions. Inhibitors for these enzymes, which have low aqueous solubility, have been studied for the treatment of different conditions. This study evaluated the effects of cosolvents and detergents on cholinesterase activity, and found that propylene glycol, acetonitrile, methanol, Tween 20, Polysorbate 80 (Tween 80), polyoxyethylene 23 lauryl ether (Brij 35), and polyoxyethylene 10 oleoyl ether (Brij 96v) had the least inhibitory effects. These solubilizing agents can be considered for evaluating potential cholinesterase inhibitors with low aqueous solubility.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Alvaro A. Recoulat Angelini, J. Jeremias Incicco, Noelia A. Melian, F. Luis Gonzalez-Flecha
Summary: This study investigates the effect of sodium dodecyl sulfate (SDS) on ATPase activity, revealing that SDS can activate or inactivate enzymes at sub-denaturing concentrations. The distribution of SDS in mixed micelles of phospholipids and a non-denaturing detergent was evaluated using an iterative procedure. Higher concentrations of SDS are required to achieve the same degree of inactivation when the initial concentration of phospholipids is increased. A simple model is proposed to explain this result and estimate the Gibbs free energy change for the inactivation process and the susceptibility of the enzyme to SDS.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Engineering, Chemical
Chunyu Zhang, Yan Sun, Xiaoyan Dong
Summary: This study successfully developed a novel enzyme-polymer conjugate by covalently coupling a zwitterionic polymer with side dimethyl chains (pID) to Candida rugosa lipase (CRL). The resulting conjugates showed higher catalytic activity, thermostability, and pH tolerance compared to the native enzyme. The findings suggest that enzyme conjugation to zwitterionic polymers has promising potential for improving enzyme performance.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2022)
Article
Chemistry, Applied
Rodolpho R. C. Monteiro, Jose J. Virgen-Ortiz, Angel Berenguer-Murcia, Thays N. da Rocha, Jose C. S. dos Santos, Andres R. Alcantara, Roberto Fernandez-Lafuente
Summary: This review presents some of the latest studies on lipase A from Candida antarctica (CALA), focusing on its stability and ability to attack the sn-2 position of triglycerides, as well as its commercial availability. It discusses the cloning, production, structural facts, and applications of the enzyme, with a special emphasis on enzyme immobilization.
Article
Chemistry, Applied
Murilo Amaral-Fonseca, Roberto Morellon-Sterling, Roberto Fernandez-Lafuente, Paulo Waldir Tardioli
Summary: A new single-step saccharification and isomerization process using immobilized commercial enzymes has been proposed in this study, achieving high efficiency in high fructose syrup production by optimizing pH, temperature, and enzyme ratio. Experimental results show that this method not only improves yield, but also reduces catalyst loss, with good recyclability.
Article
Chemistry, Applied
Rodolpho R. C. Monteiro, Jose J. Virgen-Ortiz, Angel Berenguer-Murcia, Thays N. da Rocha, Jose C. S. dos Santos, Andres R. Alcantara, Roberto Fernandez-Lafuente
Summary: This review presents some of the latest studies on lipase A from Candida antarctica (CALA), focusing on its stability and ability to attack the sn-2 position of triglycerides, as well as its commercial availability. It discusses the cloning, production, structural facts, and applications of the enzyme, with a special emphasis on enzyme immobilization.
Article
Chemistry, Applied
Keysson V. Fernandes, Elisa D. C. Cavalcanti, Eliane P. Cipolatti, Erika C. G. Aguieiras, Martina C. C. Pinto, Fernanda A. Tavares, Priscila R. da Silva, Roberto Fernandez-Lafuente, Sara Arana-Pena, Jose Carlos Pinto, Charles L. B. Assuncao, Jose Andre C. da Silva, Denise M. G. Freire
Summary: This study successfully synthesized biolubricants from by-products of soybean oil processing using the lipase from Candida rugosa, showing high conversion rates and improved efficiency through immobilization. The products demonstrated good lubricant properties, indicating a potential environmentally friendly alternative to petroleum-based lubricants.
Article
Chemistry, Applied
Sara Arana-Pena, Nathalia S. Rios, Diego Carballares, Luciana R. B. Goncalves, Roberto Fernandez-Lafuente
Summary: CALB and CRL showed similar properties under different immobilization conditions, while RML displayed significant changes. The effects of immobilization conditions on substrate activity and stability vary greatly, requiring careful selection based on specific circumstances.
Article
Chemistry, Applied
Mariane Gonsalves Santos, Diailison Teixeira de Carvalho, Lucas Belga Caminitti, Bruna Bueno Alves de Lima, Marcello Henrique da Silva Cavalcanti, Daniel Felipe Rocha dos Santos, Luciano Sindra Virtuoso, Daniela Battaglia Hirata, Eduardo Costa Figueiredo
Summary: Fe3O4@TEOS@BSA nanoparticles modified with tetraethyl orthosilicate and bovine serum albumin are efficient in separating lysozyme from egg white at different pH conditions. The extracted lysozyme maintains high enzymatic activity. These particles are easily recyclable and can be reused multiple times.
Article
Biotechnology & Applied Microbiology
Luis Fernando S. Santos, Milena R. L. Silva, Eric E. A. Ferreira, Rafaela S. Gama, Ana Karine F. Carvalho, Jayne C. S. Barboza, Jaine H. H. Luiz, Adriano A. Mendes, Daniela B. Hirata
Summary: The study successfully produced decyl oleate ester via enzymatic synthesis using a low-cost and homemade derivative prepared from chemically modified rice husks and Geotrichum candidum lipase. Optimal conditions for decyl oleate production were determined through central composite rotational design, with excellent purity and structure confirmed by H-1 NMR and IR spectroscopy.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2021)
Article
Agricultural Engineering
Wagner C. A. Carvalho, Jaine H. H. Luiz, Roberto Fernandez-Lafuente, Daniela B. Hirata, Adriano A. Mendes
Summary: Trimethylolpropane triesters (TMPTEs) were produced through enzymatic hydroesterification of refined soybean oil (RSO) or used soybean cooking oil (USCO). Immobilized Eversa (R) Transform 2.0 showed the highest activity, retaining above 90% of its original activity after 13 consecutive reaction cycles. The produced TMPTEs exhibited good cold temperature properties with pour point values between -11 and -9 degrees C.
BIOMASS & BIOENERGY
(2021)
Article
Food Science & Technology
Karen Chavez-Zaragoza, Alejandro Morales-Guerrero, Citlali Colin-Chavez, Luis Tovar-Diaz, Jose de Jesus Ornelas-Paz, Juan A. Osuna-Castro, Irasema Vargas-Arispuro, Miguel A. Martinez-Tellez, Jose J. Virgen-Ortiz
Summary: Blue LED treatment delays the senescence processes in mangoes, improves their antioxidant capacity and nutritional status, and enhances the activity of antioxidant enzymes.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Gustavo F. Oliveira, Milena R. L. Silva, Daniela B. Hirata
Summary: This study aimed to produce a new extracellular lipase from the endophytic fungus Preussia africana isolated from red lapacho tree, with optimal production achieved using sunflower oil at a C/N ratio of 8.63. The characterized lipase showed higher activity for oils with higher percentage of unsaturated fatty acids and exhibited consistent catalytic activity across a range of temperatures from 20 to 54 degrees C, making it potentially suitable for industrial applications.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2022)
Article
Microbiology
Dajara M. B. Moreira, Omar C. Gomez, Daniela B. Hirata, Jaine H. H. Luiz
Summary: Lasiodiplodia endophytes have antimicrobial and antifungal activities and provide a reliable approach for the production of high-value bioactive molecules. The yield of EtOAc extract can be improved by optimizing cultivation conditions.
CURRENT MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Camila S. Sampaio, Joelise A. F. Angelotti, Roberto Fernandez-Lafuente, Daniela B. Hirata
Summary: This review focuses on the preparation and application of cross-linked enzyme aggregates (CLEAs) from lipases. CLEAs offer advantages such as simplicity, the ability to use crude enzyme extracts, and stability under extreme conditions. However, there are challenges in the preparation process, which can be addressed through improved cross-linking methods and the design of magnetic-CLEAs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Natalia B. Machado, Guilherme J. Sabi, Daniela B. Hirata, Adriano A. Mendes
Summary: The study developed an enzymatic process for producing wax esters using immobilized lipase from TLL. The TLL-Octyl-SiO2 biocatalyst exhibited high catalytic activity and reusability, making it promising for wax ester production.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Thiago M. M. Da Cunha, Adriano A. A. Mendes, Daniela B. B. Hirata, Joelise A. F. Angelotti
Summary: This study aimed to find the optimal conditions for immobilizing a non-commercial beta-glucosidase from Aspergillus niger via cross-linked enzyme aggregates (CLEAs). The catalytic performance of the immobilized enzyme was compared with that of the soluble enzyme in terms of pH and temperature. The results showed that the immobilized enzyme had improved stability in a wider pH and temperature range, as well as enhanced thermal stability.
Article
Food Science & Technology
Irma Ofelia Maya-Meraz, Jose de Jesus Ornelas-Paz, Jaime David Perez-Martinez, Alfonso A. Gardea-Bejar, Claudio Rios-Velasco, Saul Ruiz-Cruz, Juan Ornelas-Paz, Ramona Perez-Leal, Jose Juan Virgen-Ortiz
Summary: This study found that spraying 'Shiraz' vines with CaCO3-rich residues from the cement industry can increase fruit yield, improve color development, and enhance the content of phenolic compounds and antioxidant capacity in grapes and wine.
Article
Food Science & Technology
Citlali Colin-Chavez, Jose J. Virgen-Ortiz, Marco A. Miranda-Ackerman, Orlando Hernandez-Cristobal, Miguel Angel Martinez-Tellez, Fabiola Esquivel-Chavez, Nancy Lissete Gallegos-Santoyo
Summary: Active sachets containing oregano oil microencapsulated with starch/agave fructans were developed to control phytopathogens in avocados and reduce postharvest decay. The sachets inhibited the growth of multiple phytopathogens and successfully reduced anthracnose infection in avocados without negative effects on fruit quality. In addition, the sachets increased the activity of antioxidant enzymes and the contents of phenolic compounds in treated avocados.
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)