期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 102, 期 -, 页码 9-15出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2017.03.007
关键词
Schizochytrium; Butanol; Squalene; Carotenoids; Astaxanthin
资金
- Key Program for International S&T Cooperation Projects of China [2014DFA61040]
- Hi-Tech Research and Development Program (863) of China [2014AA021701]
- Youth Innovation Promotion Association CAS
Schizochytrium is a microalgae-like fungus and is widely used for producing docosahexaenoic acid (DHA). It is also a promising source of squalene and carotenoids. However, few fermentation strategies are available in enhancing squalene and carotenoid content in Schizochytrium. This study showed that butanol addition had multiple effects on Schizochytrium limacinum B4D1. First, butanol addition altered the lipid content of cells. Second, 6 g/L of butanol decreased the proportion of DHA by nearly 40%. Third, the squalene content increased 31-fold in the presence of 6 g/L butanol. Finally, cells accumulated more carotenoids upon butanol addition. Specifically, when cells were treated with 8 g/L butanol, the astaxanthin content increased to 245 times than that of the untreated control. These results are helpful for the commercial exploitation of Schizochytrium in producing squalene and carotenoids. (C) 2017 Elsevier Inc. All rights reserved.
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