Article
Environmental Sciences
Nazim Husain, Samra Hasan, Aijaz Ahmed Khan, Riaz Mahmood
Summary: Oral administration of copper chloride in rats led to significant oxidative damage in the intestine, affecting cellular metabolism, cell membrane, and DNA damage, which in turn can impair digestive and absorptive functions.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Harvey Boughey, Mateusz Jurga, Sherif F. El-Khamisy
Summary: As we age, DNA mutations can lead to cellular dysfunction and aging, while naked mole rats show minimal biological decline and neurodegeneration despite their long lifespan.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nikhil Maheshwari, Aijaz Ahmed Khan, Riaz Mahmood
Summary: Pentachlorophenol (PCP), widely used in biocide and pesticide industries, causes oxidative damage to rat kidneys by increasing reactive oxygen species (ROS) and inhibiting antioxidant enzymes. PCP treatment also results in DNA damage, dysfunction of brush border membrane (BBM) enzymes, and increased plasma levels of creatinine and urea. These findings highlight the significance of PCP-induced oxidative stress in nephrotoxicity.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Cell Biology
Wei Zhao, Zhongjie Liu, Jiaming Luo, Changqing Ma, Luying Lai, Zhengyuan Xia, Shiyuan Xu
Summary: The study indicates that PARP-1 plays a key role in repairing oxidative DNA damage induced by bupivacaine, with interactions with XPD protein contributing to DNA integrity maintenance.
Article
Environmental Sciences
Sonalin Rath, Surajit Das
Summary: Bacteria thriving in the mangrove ecosystem play a significant role in elemental cycles, but their performance is influenced by climate change and environmental stressors. This study investigates the genotoxic effect of such stressors on mangrove bacteria and their adaptation strategies. The results show cellular injuries and DNA damage in response to stressors, with different bacterial strains exhibiting varying levels of tolerance and adaptive mechanisms.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Merle M. Nicolai, Ann-Kathrin Weishaupt, Jessica Baesler, Vanessa Brinkmann, Anna Wellenberg, Nicola Winkelbeiner, Anna Gremme, Michael Aschner, Gerhard Fritz, Tanja Schwerdtle, Julia Bornhorst
Summary: Although manganese is an essential trace element, overexposure can lead to toxicity and neurological dysfunction. This study investigated the mode of action of manganese toxicity on genomic integrity, focusing on DNA damage and repair mechanisms using Caenorhabditis elegans as a model organism. The results suggest that excessive manganese exposure can decrease genomic integrity and activate base excision repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nazim Husain, Shaikh Nisar Ali, Hussain Arif, Aijaz Ahmed Khan, Riaz Mahmood
Summary: The effect of CuCl2 on oxidative stress, cellular metabolism, and DNA integrity in rat kidney was investigated. CuCl2 was found to impair the antioxidant defense system, leading to oxidative damage in the kidney.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dandan Sun, Shimei Chen, Shenping Li, Ning Wang, Shuchang Zhang, Li Xu, Shaopin Zhu, Huiming Li, Qing Gu, Xun Xu, Fang Wei
Summary: The accumulation of DNA damage induced by oxidative stress is a crucial pathogenic factor of endothelial loss in diabetic vascular complications. It is still unclear whether aberrant glucose metabolism leads to defective DNA repair and accounts for hyperglycemia-induced endothelial oxidative stress injury. Our study showed that Foxo1 knockdown alleviated diabetes-associated retinal DNA damage and vascular dysfunction by promoting DNA repair mediated by the MRN-ATM pathway and restoring glycolysis capacity. Our findings suggest that defective glycolysis-dependent DNA repair could be a novel therapeutic target for diabetic vascular complications.
Review
Geriatrics & Gerontology
S. G. Chowdhury, S. Misra, Parimal Karmakar
Summary: Ageing is a multi-factorial phenomenon, and accumulation of DNA damage or mutations is a dominant theory which contributes largely to the development of ageing and age-related diseases. Obesity is a complex chronic disease that affects multiple organs and tissues, and it has been found to be associated with higher loads of DNA damage. The present review focuses on the involvement of DNA metabolism in the development of obesity and how it accelerates ageing in mammals.
JOURNAL OF NUTRITION HEALTH & AGING
(2023)
Article
Biochemistry & Molecular Biology
Galina N. Chelomina, Sergey P. Kukla, Viktor P. Chelomin, Pham N. Doanh
Summary: Parasite infection can lead to DNA damage, potentially involving oxidative stress and cancer development; these research findings are important for understanding the pathogenesis mechanisms of parasitic diseases.
Review
Biochemistry & Molecular Biology
Elle E. M. Scheijen, Sven Hendrix, David M. Wilson
Summary: Oxidative stress plays a crucial role in spinal cord injury (SCI), causing damage to proteins, nucleic acids, lipids, cytoskeleton, and organelles. Oxidative DNA damage is likely a major contributor to the pathogenesis of SCI, although the evidence supporting this hypothesis is limited. Improved analysis methods are needed to uncover the role of DNA damage and repair in SCI.
Article
Gastroenterology & Hepatology
Verena von Buelow, Sarah Gindner, Anne Baier, Laura Hehr, Nicola Buss, Lena Russ, Sarah Wrobel, Victoria Wirth, Kuscha Tabatabai, Thomas Quack, Simone Haeberlein, Patrik Kadesch, Stefanie Gerbig, Katja R. Wiedemann, Bernhard Spengler, Annabel Mehl, Gertrud Morlock, Gabriele Schramm, Joern Pons-Kuehnemann, Franco H. Falcone, R. Alan Wilson, Katrin Bankov, Peter Wild, Christoph G. Grevelding, Elke Roeb, Martin Roderfeld
Summary: In this study, it was demonstrated that soluble egg products of the parasite S. mansoni completely reprogram lipid and carbohydrate metabolism, causing oxidative stress-induced cell damage in the host parenchyma. The eggs take advantage of the host environment through metabolic reprogramming of hepatocytes and enterocytes, and induce DNA damage, promoting hepatocellular damage. These findings have important implications for international health efforts.
Article
Endocrinology & Metabolism
Jay Kumar, Ghulam Mohammad, Kumari Alka, Renu A. Kowluru
Summary: Mitochondrial dysfunction plays a critical role in the development of diabetic retinopathy. Hyperglycemia downregulates the mtDNA-encoded LncRNA cytochrome B (LncCytB) in retinal endothelial cells, leading to impaired mtDNA packaging and increased vulnerability to damage.
Article
Plant Sciences
Neha Gupta, Arun Kumar Mishra
Summary: NOS-derived NO plays a critical role in protecting cyanobacteria from oxidative burst and maintaining their physiological balance. Addition of L-NAME inhibitor reduces NO accumulation, while addition of L-arginine increases NO synthesis. Inhibition of NOS leads to decreased growth and metabolic activity of cyanobacteria, as well as decline in photopigments, PSII efficiency, loss in membrane integrity and DNA damage, ultimately resulting in cell death.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Oncology
Sepideh Nikfarjam, Krishna K. Singh
Summary: DNA damage response (DDR) signaling is crucial for maintaining genomic and proteomic homeostasis and dysregulation in DDR pathways is associated with various pathophysiological states, including cancer and cardiovascular diseases (CVDs). This review comprehensively discusses different DDR pathways, the cross talk among DDR mechanisms, the role of DDR in cancer and CVDs, the similarities and differences between DDR in cancer and CVDs, the role of DDR in the pathophysiology of CVDs, interventional strategies for targeting genomic instability in CVDs, and future perspectives.
Article
Chemistry, Analytical
Lei Guo, Jiyang Dong, Xiangnan Xu, Zhichao Wu, Yinbin Zhang, Yongwei Wang, Pengfei Li, Zhi Tang, Chao Zhao, Zongwei Cai
Summary: Research on metabolic heterogeneity is crucial for understanding the molecular mechanisms of diseases and developing personalized treatments. The use of mass spectrometry imaging (MSI) enables the identification of spatially contiguous or sporadic regions of interest, revealing the heterogeneity of an organism at a molecular level.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Li Xiang, Yi Ru, Jingchun Shi, Li Wang, Hongzhi Zhao, Yu Huang, Zongwei Cai
Summary: In this study, a simple and sensitive method for the detection of N-acyl glycines (NAGlys) in plasma and urine samples using liquid chromatography-mass spectrometry (LC-MS) is described. The use of a derivatization reagent, 3-nitrophenylhydrazine, allows for quick reaction in aqueous solution without the need for a quenching step. NAGlys were first identified based on high-resolution LC-MS and subsequently quantified on triple quadrupole LC-MS. This approach enables a broader measurement of NAGlys and potential identification of diagnostic biomarkers for type II diabetes and diabetic complications.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Xiaochong He, Qiting Zhao, Xuyang Chai, Yuanyuan Song, Xuelan Li, Xingwen Lu, Shoupeng Li, Xin Chen, Yong Yuan, Zongwei Cai, Zenghua Qi
Summary: A study was conducted to analyze the concentration and chemical speciation of PM2.5-bound lead (PM2.5-Pb) at a non-ferrous metal smelting site. It was found that PM2.5-Pb exposure has a significant impact on human absorption of lead and can cause cardiovascular damage. Animal simulation experiments were used to evaluate the relationship between PM2.5-Pb exposure and cardiovascular damage, and a risk assessment model was established. This research provides accurate estimates of the risk of PM2.5-bound heavy metals on the cardiovascular system and offers a scientific basis for future prevention and therapy of PM2.5-Pb-related diseases.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Hongli Tan, Liu Yang, Xiaolin Liang, Diedie Huang, Xinhang Qiao, Qingyuan Dai, Da Chen, Zongwei Cai
Summary: With the tightening restrictions on phthalate esters (PAEs), nonphthalate plasticizers (NPPs) are increasingly used. However, there is limited knowledge on the environmental occurrences, fate, and human exposure risks of many emerging NPPs. This study investigated the presence of NPPs and major PAEs in house dust, finding widespread occurrences of NPPs, such as ATBC, TCTM, TOTM, GMO, MO, and DiBA. Although current exposure may not pose significant health risks, potential hazards cannot be overlooked due to the lack of toxic threshold data and possible cocktail effects.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Editorial Material
Engineering, Environmental
Guodong Cao, Zongwei Cai
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Jing Chen, Peisi Xie, Qingyuan Dai, Pengfei Wu, Yu He, Zian Lin, Zongwei Cai
Summary: In this study, MALDI-2 and TIMS imaging were used to analyze lipids and metabolites in H295R adrenocortical MCTS. MALDI-2 detected more lipids and metabolites in MCTS compared to traditional MALDI, while TIMS successfully separated isomeric and isobaric ions in different regions of MCTS. MS/MS imaging experiments further confirmed the presence of these isomeric and isobaric ions. These findings demonstrate the potential of MALDI-2 and TIMS imaging as valuable tools for lipid and metabolite analysis in MCTS for various biological and medical research fields.
Article
Engineering, Environmental
Guodong Cao, Wei Wang, Jing Zhang, Pengfei Wu, Han Qiao, Huankai Li, Gefei Huang, Zhu Yang, Zongwei Cai
Summary: para-Phenylenediamine quinones (PPD-Qs) are newly discovered transformation products derived from para-phenylenediamine (PPD) antioxidants. Their occurrence and fate in wastewater treatment plants (WWTPs) need further investigation to improve pollutant removal efficiency.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Jie-Ru Jiang, Zhi-Feng Chen, Xiao-Liang Liao, Qian-Yi Liu, Jia-Ming Zhou, Shi-Ping Ou, Zongwei Cai
Summary: Tire wear particles (TWPs) are increasingly present in water, but little is known about their environmental consequences. In this study, TWP leachates were analyzed to identify potentially toxic organic substances using high-resolution mass spectrometry and toxicity identification. Thirteen substances were structurally identified, with cyclic amines found to be toxic to the tested algal species. Metabolomic analysis revealed differential metabolites associated with linoleic acid and purine metabolism, and oxidative stress was identified as a key factor in algal growth inhibition. This study highlights the risk posed by TWP leachable substances to aquatic organisms.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Environmental
Yanshan Liang, Leiguang Li, Hongna Zhang, Qingyuan Dai, Guangshan Xie, Bo Lei, Zhu Yang, Zongwei Cai
Summary: Triclosan can cause thyroid damage and disrupt the synthesis and release of thyroid hormones, leading to hypothyroidism.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Analytical
Gefei Huang, Feng Zhang, Dongying Xie, Yiming Ma, Pengxi Wang, Guodong Cao, Leijian Chen, Siyi Lin, Zhongying Zhao, Zongwei Cai
Summary: In this study, a high-throughput analytical platform combining ultra-performance liquid chromatography (UPLC) and complementary mass spectrometry (MS) was developed to identify and quantify RNA modifications. Sixty-four types of RNA modifications were successfully monitored within a 16-minute single run of UPLC-MS. The method can also aid in the discovery of RNA-modifying enzymes in genetic studies.
Article
Engineering, Environmental
Lin Zhu, Lei Du, Guodong Cao, Zongwei Cai
Summary: Existing and emerging persistent organic pollutants (POPs) pose significant threats to human health and economies. The development of effective mitigation strategies to remove or degrade these chemicals in the environment is crucial due to their persistence. Although various methods for electrocatalytic degradation of POPs have been developed in lab conditions, challenges such as harmful byproducts and limited working time hinder their practical application. This perspective work highlights high-entropy alloys as ideal catalyst materials for electrocatalytic degradation and discusses the use of AI to facilitate the design and testing of electrocatalysts. The challenges, negative effects, and solutions of applying AI in this process are also addressed.
ENVIRONMENTAL SCIENCE-ADVANCES
(2023)
Article
Biochemical Research Methods
Mei Xie, Tianlan Chen, Zongwei Cai, Bo Lei, Cheng Dong
Summary: Traditional diagnosis of infectious diseases relies on advanced analyzers in central hospitals, which is not sufficient for rapid control of epidemics, especially in resource-limited areas, raising the importance of the development of diagnostic systems for point-of-care testing (POCT). Here, researchers developed a simple and cost-effective digital microfluidic (DMF) platform in combination with colorimetric loop-mediated isothermal amplification (LAMP) for simple on-site diagnosis of diseases by the naked eye. The DMF-LAMP assay showed comparable sensitivity to conventional LAMP assay but was more efficient, and possessed a simple chip structure and high flexibility to integrate multiplex analysis.
Article
Chemistry, Analytical
Li Xiang, Yi Ru, Jingchun Shi, Hongzhi Zhao, Yu Huang, Zongwei Cai, Li Wang
Summary: N-Acyl glycines (NAGlys) are important metabolites in the human body's detoxification system, and can be used in the diagnosis of metabolic diseases. A simple and sensitive method of NAGlys detection by LC-MS in plasma and urine samples is described, using 3-nitrophenylhydrazine as a derivatization reagent. This approach allows for a broader measurement of NAGlys, especially in the absence of authentic standards. The identified NAGlys have the potential to be diagnostic biomarkers for type II diabetes and diabetic complications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Peige Qin, Shiping Zhu, Mengyao Mu, Yanmei Gao, Zongwei Cai, Minghua Lu
Summary: A new hierarchical mixed-dimensional metal-organic framework (MOF@MOF) hybrid material was prepared for the removal and enrichment of environmental pollutants. The material exhibited excellent adsorption performance for bisphenols (BPs) due to its extensive mesoporous structures and large surface area.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Jiajing Chen, Huan Huang, Dan Ouyang, Jiali Lin, Zhuling Chen, Zongwei Cai, Zian Lin
Summary: Analysis of cis-diol compounds is crucial due to their important roles in various fields. Researchers have developed a matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS) method to effectively analyse cis-diol compounds. This method utilizes a unique reactive matrix (BMQI) for in situ derivatization of cis-diol compounds, resulting in improved speed, reproducibility, and signal intensity, as well as reduced background interference. Additionally, the method has been successfully applied to the rapid screening and identification of sugar alcohols in different sugar-free foods.
