期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 51, 期 6, 页码 3391-3401出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.6b04366
关键词
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资金
- National Natural Science Foundation of China [41322008, 41673111]
- Doctoral Scientific Research Foundation of Guangdong Province [2016A030310118]
The indigenous microorganisms responsible for degrading phenanthrene (PHE) in polycyclic aromatic hydrocarbons (PAHs)-contaminated wastewater were identified by DNA-based stable isotope probing (DNA-SIP). In addition to the well-known PHE degraders Acinetobacter and Sphingobium, Kouleothrix and Sandaracinobacter were found, for the first time, to be directly responsible for indigenous PHE biodegradation. Additionally, a novel PHE degrader, Acinetobacter tandoii sp. LJ-5, was identified by DNA-SIP and direct cultivation. This is the first report and reference to A. tandoii involved in the bioremediation of PAHs-contaminated water. A PAH-RHD alpha gene involved in PHE metabolism was detected in the heavy fraction of C-13 treatment, but the amplification of PAH-RHD alpha gene failed in A. tandoii LJ-5. Instead, the strain contained eatechol 1,2-dioxygenase and alpha/beta subunits of protocatechuate 3,4-dioxygenase, indicating use of the-beta-ketoadipate pathway to degrade PHE and related aromatic compounds. These findings add to our current knowledge on microorganisms degrading PHE by combining cultivation-dependent and cultivation-independent approaches and provide deeper insight into the diversity of indigenous PHE-degrading communities.
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