期刊
ENVIRONMENTAL MICROBIOLOGY
卷 19, 期 2, 页码 788-802出版社
WILEY
DOI: 10.1111/1462-2920.13648
关键词
-
类别
资金
- Natural Science Foundation of Chongqing [CSTC2013jcyjA80030]
- Fundamental Research Funds for the Central Universities [XDJK2015B032, XDJK2016E107]
- National Science Foundation of China [31171902]
- US-National Science Foundation [IOS-1557704]
- National Council for Scientific and Technological Development (CNPq) of Brazil [PQ1D 308436/20148]
- Sao Paulo Research Foundation (FAPESP) [2010/06374-1, 2013/50518-6]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1557704] Funding Source: National Science Foundation
The PacC transcription factor is an important component of the fungal ambient pH-responsive regulatory system. Loss of pacC in the insect pathogenic fungus Beauveria bassiana resulted in an alkaline pH-dependent decrease in growth and pH-dependent increased susceptibility to osmotic (salt, sorbitol) stress and SDS. Extreme susceptibility to Congo Red was noted irrespective of pH, and BbpacC conidia showed subtle increases in UV susceptibility. The BbPacC mutant showed a reduced ability to acidify media during growth due to failure to produce oxalic acid. The BbPacC mutant also did not produce the insecticidal compound dipicolinic acid, however, production of a yellow-colored compound was noted. The compound, named bassianolone B, was purified and its structure determined. Despite defects in growth, stress resistance, and oxalate/insecticidal compound production, only a small decrease in virulence was seen for the BbpacC strain in topical insect bioassays using larvae from the greater waxmoth, Galleria mellonella or adults of the beetle, Tenebrio molitor. However, slightly more pronounced decreases were seen in virulence via intrahemcoel injection assays (G. mellonella) and in assays using T. molitor larvae. These data suggest important roles for BbpacC in mediating growth at alkaline pH, regulating secondary metabolite production, and in targeting specific insect stages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据