Article
Ecology
Mei Li, Thomas Pommier, Yue Yin, Jianing Wang, Shaohua Gu, Alexandre Jousset, Joost Keuskamp, Honggui Wang, Zhong Wei, Yangchun Xu, Qirong Shen, George A. Kowalchuk
Summary: The rhizosphere microbiome plays a crucial role in defending against soilborne pathogens, and inhibiting the growth of pathogen helpers may be effective in reducing pathogen densities. This approach could potentially become a sustainable strategy for pathogen control.
Article
Horticulture
Rachidatou Sikirou, Marie Epiphane Dossoumou, Judith Honfoga, Victor Afari-Sefa, Ramasamy Srinivasan, Mathews Paret, Wubetu Bihon
Summary: Bacterial wilt caused by Ralstonia solanacearum is a growing concern in amaranth production in Benin. Host resistance is identified as the most sustainable disease control measure. The study suggests that the UG-AMES13-2 variety from the World Vegetable Center shows high resistance to R. solanacearum and should be promoted for seed production to support farmers.
Article
Plant Sciences
Pau Sebastia, Roger de Pedro-Jove, Benoit Daubech, Anurag Kashyap, Nuria S. Coll, Marc Valls
Summary: This study systematically analysed the infection of R. solanacearum in S. dulcamara, showing that bittersweet plants exhibit partial resistance to bacterial wilt compared with susceptible hosts like tomatoes, especially at lower temperatures. The findings suggest that inherent resistance and perennial growth habits enable bittersweet plants to act as reservoirs for the pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Wenlong Liang, Jingli Cheng, Jiadong Zhang, Qiuyu Xiong, Maojun Jin, Jinhao Zhao
Summary: By utilizing a core-shell nanocarrier that releases the bactericide berberine in an acidic environment, this study demonstrates an effective control of tomato bacterial wilt disease without negative impact on plant growth.
Article
Agriculture, Multidisciplinary
Keke Dang, Jinfeng Hou, Hong Liu, Junwei Peng, Yang Sun, Jiangang Li, Yuanhua Dong
Summary: In this study, the changes in rhizobacteria, endobacteria, and root exudates during the pathogenesis of bacterial wilt in ginger caused by Ralstonia solanacearum (Rs) were analyzed using high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). The results showed that Rs infection led to a 50.00% incidence of bacterial wilt in ginger and altered the composition of bacterial communities in both the endosphere and rhizosphere. Furthermore, the changes in rhizobacterial composition induced corresponding changes in endobacterial and root exudate compositions. The upregulated exudates inhibited ginger bacterial wilt, indicating that ginger produces antibacterial compounds as a defense mechanism against bacterial pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Xuhui Deng, Na Zhang, Zongzhuan Shen, Chengzhi Zhu, Hongjun Liu, Zhihui Xu, Rong Li, Qirong Shen, Joana Falcao Salles
Summary: Manipulation of the soil microbiome composition can enhance soil resistance to pathogens, leading to a natural suppressive effect. This provides a promising strategy for disease control by reshaping the soil microbiome.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Biochemistry & Molecular Biology
Corri D. Hamilton, Olivia R. Steidl, April M. MacIntyre, Connor G. Hendrich, Caitilyn Allen
Summary: The soilborne pathogen Ralstonia solanacearum infects host roots and colonizes xylem vessels, utilizing different carbon sources at low cell density (LCD) and high cell density (HCD) stages to adapt to the nutritional environments of plant roots and xylem sap, thereby influencing its growth and infection in plants.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Lanping Shi, Xia Li, Yahong Weng, Hanyang Cai, Kaisheng Liu, Baixue Xie, Hussain Ansar, Deyi Guan, Shuilin He, Zhiqin Liu
Summary: The study reveals that plant immunity and dehydration tolerance are coupled and regulated by the CaPti1-CaERF3 module in pepper plants, enhancing resistance against bacterial wilt caused by Ralstonia solanacearum.
Article
Multidisciplinary Sciences
Dingxin Wen, Qingqing Guo, Wan Zhao, Yong Yang, Chunlei Yang, Jun Yu, Yun Hu
Summary: The study found that hydrogen sulfide can prevent tobacco bacterial wilt. The results showed that hydrogen sulfide can inhibit the growth of the bacteria, alter its cell morphology, inhibit biofilm formation and swarming motility, and reduce the population of bacteria invading tobacco roots.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Sampurna Kashyap, Indrani Sharma, Bhaskar Dowarah, Ramen Barman, Sarvajeet Singh Gill, Niraj Agarwala
Summary: Plant and soil-associated microbiome play a crucial role in conferring tolerance to bacterial wilt disease in plants. Ralstonia solanacearum (RS), the causative agent of bacterial wilt, colonizes the xylem vessels and restricts water supply, leading to wilting. This review highlights the dynamics of microbiome associated with bacterial wilt disease and provides an update on microbial and non-microbial approaches utilized for disease control in crop plants.
Article
Plant Sciences
Shili Li, Jing Pi, Hongjiang Zhu, Liang Yang, Xingguo Zhang, Wei Ding
Summary: Caffeic acid is induced and exhibits antibacterial activity in tobacco plants infected by Ralstonia solanacearum, playing a crucial role in defending against pathogen infection and controlling bacterial wilt. It damages the membrane structure of R. solanacearum cells, inhibits biofilm formation, and promotes the accumulation of lignin and hydroxyproline, ultimately reducing and delaying the incidence of tobacco bacterial wilt.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Liang Yang, Zhouling Wei, Shili Li, Rui Xiao, Qinqin Xu, Yuao Ran, Wei Ding
Summary: The plant secondary metabolite daphnetin inhibits Ralstonia solanacearum extracellular polysaccharides production and biofilm formation, reducing its virulence on tobacco plants. Daphnetin alters gene expression of EPS synthesis cluster and virulence factors, impacting the pathogen's ability to infect plants effectively. Molecular docking studies suggest daphnetin interacts with critical residues of EpsB protein through hydrogen bonding and hydrophobic interactions, highlighting its potential as a novel virulence inhibitor.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Microbiology
Leo Gerlin, Antoine Escourrou, Cedric Cassan, Felicia Maviane Macia, Nemo Peeters, Stephane Genin, Caroline Baroukh
Summary: The plant pathogen Ralstonia solanacearum proliferates rapidly in xylem vessels, leading to plant death at a tipping point in bacterial wilt dynamics. Metabolic and physiological changes during infection are similar to drought stress. Glutamine is identified as the preferred substrate for R. solanacearum during colonization.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Keming Yang, Xiaofang Wang, Rujiao Hou, Chunxia Lu, Zhe Fan, Jingxuan Li, Shuo Wang, Yangchun Xu, Qirong Shen, Ville-Petri Friman, Zhong Wei
Summary: This study investigates the influence of rhizosphere phage communities on soil suppressiveness and bacterial wilt disease. It shows that healthy plants are associated with phage communities that have stronger control over the pathogen R. solanacearum. Additionally, phages that target pathogen-inhibiting bacteria are more abundant in diseased plant microbiomes. These findings suggest that rhizosphere phage communities play a crucial role in determining bacterial wilt disease outcomes and soil suppressiveness.
Article
Horticulture
Yang Gao, Lei Wang, Rui Liu, Jihui Tian, Kunzheng Cai
Summary: This study evaluated the effects of wheat straw biochar on disease development, plant physiology, and proteomic expression in tomato plants infected with Ralstonia solanacearum. The results showed that biochar application improved plant growth, reduced disease severity, and decreased the colonization of R. solanacearum in tomato stems. Physiological analysis revealed that biochar treatment increased peroxidase and lipoxygenase activities, as well as the content of total soluble phenolics and lignin-like phenolic polymers in tomato leaves. Proteomic analysis showed that biochar treatment upregulated genes involved in lignin synthesis, anion channel and sulfur metabolism, and downregulated genes involved in microtubule and cytoskeleton organization, as well as UDP-glucose metabolism.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Xiaoli Liu, Jun Cai, Xiaoxu Li, Feng Yu, Dousheng Wu
Summary: The article hypothesizes that type III effectors manipulate the microbiota composition by mediating ternary pathogen-plant-microbiota interactions, providing new insights for studying the biological functions of type III effectors and developing new strategies for plant disease control.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Jing Tang, Dousheng Wu, Xiaoxu Li, Lifeng Wang, Ling Xu, Yi Zhang, Fan Xu, Hongbin Liu, Qijun Xie, Shaojun Dai, Devin Coleman-Derr, Sirui Zhu, Feng Yu
Summary: Phosphate starvation triggers a signaling pathway involving PHR1 and RALF genes in Arabidopsis thaliana, leading to modulation of the root microbiome composition. This interaction suppresses plant immunity through the FERONIA protein, allowing colonization by beneficial root microbiota and upregulation of phosphate starvation response genes.
Review
Microbiology
Nick C. Snelders, Hanna Rovenich, Bart P. H. J. Thomma
Summary: Fungi interact not only with their hosts but also with the host-associated microbiota, manipulating the microbiota through effector proteins to stimulate disease development. Effector-mediated microbiota manipulation is fundamental to fungal biology and occurs beyond plant pathogens, being widespread in the fungal kingdom and occurring in many contexts.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Limei Song, Guoyun Xu, Tingting Li, Huina Zhou, Qinlu Lin, Jia Chen, Long Wang, Dousheng Wu, Xiaoxu Li, Lifeng Wang, Sirui Zhu, Feng Yu
Summary: The TOR kinase is activated in plants by interacting with the receptor kinase FERONIA (FER), which helps regulate nitrogen and amino acid signaling under low-nutrient conditions and promotes leaf growth.
Article
Biotechnology & Applied Microbiology
Hong-Bin Liu, Xiaoxu Li, Jun Cai, Ling-Li Jiang, Xin Zhang, Dousheng Wu, Lifeng Wang, Aiguo Yang, Cun Guo, Jia Chen, Wenxuan Pu, Feng Yu
Summary: This study identified 33 highly effective FER kinase inhibitors that can alleviate soil-borne diseases in plants without growth penalties, providing a new strategy for the prevention and control of plant diseases.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Weijun Chen, Huina Zhou, Fan Xu, Meng Yu, Alberto Coego, Lesia Rodriguez, Yuqing Lu, Qijun Xie, Qiong Fu, Jia Chen, Guoyun Xu, Dousheng Wu, Xiushan Li, Xiaojuan Li, Yvon Jaillais, Pedro L. L. Rodriguez, Sirui Zhu, Feng Yu
Summary: In Arabidopsis, the receptor-like kinase FERONIA (FER) senses peptide ligands in the plasma membrane and is involved in plant growth, stress signaling, and adaptive responses. Our study reveals that FER interacts with C2 domain ABA-related (CAR) proteins to control the nano-organization of the plasma membrane. This interaction leads to the upregulation of CAR protein translation and stabilizes the plasma membrane's liquid-ordered phase.
Article
Plant Sciences
Paul Weiland, Felix Dempwolff, Wieland Steinchen, Sven-Andreas Freibert, Hui Tian, Timo Glatter, Roman Martin, Bart P. H. J. Thomma, Gert Bange, Florian Altegoer
Summary: Plant-pathogenic fungi play a major role in causing plant diseases and crop loss. In this study, the atomic structures of Cpl1 and Uvi2 proteins from Ustilago maydis and Ustilago hordei were determined. These proteins adopt a unique double-ψβ-barrel architecture and bind to chitin fragments in a novel mode. Cpl1 localizes to the cell wall of U. maydis and might coordinate with other cell wall-degrading and decorating proteins during maize infection. Deletion of uvi2 significantly impairs U. hordei virulence, indicating a diverging function from Cpl1.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Jun Huang, Sanzhen Liu, David E. Cook
Summary: The evolution of plant and microbial interactions is influenced by genetic variation, leading to the selection of beneficial traits. Technological advancements have expanded our understanding of the diversity of genetic variation influencing plant-microbial interactions. The dynamic genome, encompassing a wide array of adaptive traits, plays a significant role in plant and microbial evolution.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Yuanyuan Li, Junping Gao, Ying Wang, Jun Cai, Dousheng Wu, Long Wang, Wenxuan Pu, Feng Yu, Sirui Zhu
Summary: Transfer RNA (tRNA) can produce tRNA-derived fragments (tRFs) that play important roles in cellular programs. However, the functional mechanisms of tRFs in plants are largely unknown. In this study, we investigated the effects of tRF-Ala, derived from tRNA-Ala, on mRNA levels and splicing in Arabidopsis. We found that tRF-Ala interacts directly with the splicing factor SR34 and competes for its binding sites, thereby regulating mRNA expression and splicing.
Review
Biochemistry & Molecular Biology
Fantin Mesny, Stephane Hacquard, Bart P. H. J. Thomma
Summary: Plants interact with a diverse array of microorganisms that affect their growth and resilience, leading them to be considered as plant holobionts rather than singular organisms. The assembly of above- and belowground microbiota in a plant holobiont is influenced by host, microbial, and environmental factors. Plants activate immune signaling upon perceiving microorganisms, which then modulate the composition of the plant microbiota through the secretion of factors. Metabolic interdependencies and antagonism between microbes are also important for shaping community assemblies. Overall, these complex interactions have evolved and play a crucial role in promoting the survival and fitness of plants and their associated microorganisms as holobionts. The co-evolution of these interactions within holobionts ultimately influences plant performance.
Editorial Material
Plant Sciences
Hanna Rovenich, Bart P. H. J. Thomma
Review
Microbiology
H. Martin Kramer, David E. Cook, Michael F. Seidl, Bart P. H. J. Thomma
Summary: Through the association of protein complexes to DNA, the eukaryotic nuclear genome is organized into open euchromatin that is accessible and condensed heterochromatin that is inaccessible. Chromatin alterations impact its organization and functionality and are important regulators of nuclear processes. Studies in fungal plant pathogens uncover the association between chromatin organization and the expression of in planta-induced genes that are important for pathogenicity.
Review
Microbiology
Jun Cai, Yuxin Jiang, Erin S. Ritchie, Alberto P. Macho, Feng Yu, Dousheng Wu
Summary: Pathogens utilize effector proteins to manipulate plant metabolism, providing nutrients, suppressing defense responses, and reshaping the microbiota to promote infection. Effector proteins are secreted to the plant apoplast or inside plant cells, where they interfere with plant immunity and cellular processes to facilitate infection. Manipulation of host metabolism by pathogen effectors is crucial for successful infection and can also impact the composition of the microbiota.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Plant Sciences
Sanzhen Liu, Guifang Lin, Sowmya R. Ramachandran, Lidia Calderon Daza, Giovana Cruppe, Batiseba Tembo, Pawan Kumar Singh, David Cook, Kerry F. Pedley, Barbara Valent
Summary: The fungal pathogen causing wheat blast, Magnaporthe oryzae Triticum pathotype, was first discovered in South America and has since spread to South Asia and Africa. Genetic studies reveal that closely related strains to a South American field isolate B71 are responsible for the outbreaks in South Asia and Africa. Analysis of the genomic variation among isolates shows that while the core genomes are highly similar, the mini-chromosomes have undergone significant diversification. The presence of these mini-chromosomes and rapid genomic changes suggests their potential importance in virulence or niche adaptation under different environmental conditions.
Review
Microbiology
Jun Huang, David E. Cook
Summary: This review summarizes and compares the molecular mechanism, hierarchy, and regulation of four DNA double-strand break repair pathways in animal and fungal models, and connects them to genome engineering outcomes and biased genome evolution in filamentous pathogens.
FEMS MICROBIOLOGY REVIEWS
(2022)
