期刊
ENERGY
卷 139, 期 -, 页码 962-974出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.energy.2017.08.052
关键词
Oleaginous yeast; Lipid accumulation; Fatty acid methyl esters; Biodiesel properties
资金
- DBT, Govt. of India
- Bio Care Programme, DBT [102/IFD/SAN/3539/2011-2012, DBT-608-BIO]
- SRF from UGC, India [6405-35-044]
In this study oleaginous yeast, Rhodosporidium kratochvilovae HIMPA1 grown in glucose synthetic medium containing different nitrogen (1 and 0.1 g/l) and phosphorus (0.05 and 0.1 g/l) limited conditions. Among various N and P-limited conditions, the highest lipid content (60.34 +/- 0.69%) obtained under synchronized limitation of N and P (0.1 g/l N and 0.05 g/l P). Live fluorescent cell imaging of yeast cells after BODIPY505-515 nm staining endorses the results of triacylglycerol (TAG) accumulation in lipid droplets. The cells grown in synchronized limitation of N and P exhibited boosted cell size (6.76 +/- 0.39 mu m) and lipid droplet size (5.62 +/- 0.28 mu m). Under synchronized limitation of N and P, supersized irregular shaped lipid droplets (LD) coalesced to form big lobules in the cellular compartment of oleaginous yeast having 87.14% enhanced TAG accumulation as depicted by TLC. Synchronized nutrient limitation diverts the CDP-DG pathway of phospholipids synthesis towards de novo TAG synthesis. The maximum increment of oleic acid (C18:1) was reported in synchronized limitation of N and P that improve the biodiesel properties like oxidative stability, viscosity, cetane number and cold filter plugging point. (C) 2017 Elsevier Ltd. All rights reserved.
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