Article
Multidisciplinary Sciences
Changwei Yang, Tao Sheng, Wenhui Hou, Juan Zhang, Li Cheng, Hao Wang, Wei Liu, Shiqi Wang, Xinmin Yu, Yuqi Zhang, Jicheng Yu, Zhen Gu
Summary: This article describes a transdermal polymeric microneedle patch that achieves glucose-responsive closed-loop insulin and glucagon delivery for glycemic regulation with minimized risk of hypoglycemia. The patch utilizes glucose-responsive phenylboronic acid units to reversibly shift the net charge of the polymeric matrix, allowing dynamic tuning of insulin and glucagon analog release ratio to achieve glycemic homeostasis.
Article
Endocrinology & Metabolism
Xi Chen, Enrique Maldonado, Ralph A. DeFronzo, Devjit Tripathy
Summary: The study revealed that there is a relationship between plasma glucagon levels and insulin sensitivity and insulin secretion in obese individuals. As glucose intolerance worsens, the insulin-mediated suppression of glucagon secretion is impaired, which corresponds to the severity of insulin resistance and beta-cell dysfunction.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Chemistry, Analytical
Mika Hatada, Ellie Wilson, Mukund Khanwalker, David Probst, Junko Okuda-Shimazaki, Koji Sode
Summary: Diabetes is a rapidly growing global health emergency, and comprehensive management is necessary for patients. Therefore, the development of diabetes POCT sensors for real-time testing is important for the management of diabetic patients.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
May-Yun Wang, E. Danielle Dean, Ezekiel Quittner-Strom, Yi Zhu, Kamrul H. Chowdhury, Zhuzhen Zhang, Shangang Zhao, Na Li, Reshing Ye, Young Lee, Yiyi Zhang, Shiuhwei Chen, Xinxin Yu, Derek C. Leonard, Greg Poffenberger, Alison Von Deylen, S. Kay McCorkle, Amnon Schlegel, Kyle W. Sloop, Alexander M. Efanov, Ruth E. Gimeno, Philipp E. Scherer, Alvin C. Powers, Roger H. Unger, William L. Holland
Summary: The monoclonal antibody antagonist Ab-4 showed promising results in maintaining glucose homeostasis in type 1 diabetic rodents, promoting beta-cell survival and enhanced insulin* islet mass recovery. This treatment also significantly increased beta-cell mass and improved C-peptide levels in diabetic mice, even driving alpha-cell-to-beta-cell conversion for functional insulin-producing cell restoration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Physiology
Klinsmann Carolo Dos Santos, Camilla Olofsson, Joao Paulo M. C. M. Cunha, Fiona Roberts, Sergiu-Bogdan Catrina, Malin Fex, Neda Rajamand Ekberg, Peter Spegel
Summary: The study found that insulin and glucagon secretion depended on glycemic status and meal composition, while IGR depended on meal composition. Consuming a low carbohydrate diet may be beneficial for weight loss, especially in obese individuals.
Article
Medicine, Research & Experimental
Hari Priya Vemana, Vikas V. Dukhande
Summary: Insulin and glucagon play key roles in the pathogenesis of type 2 diabetes. Our study found that insulin and glucagon affect the process of ubiquitination, mainly enriching in signaling pathways such as MAPK, Akt, and oxidative stress.
Article
Cell Biology
A. Tate Lasher, Liou Y. Sun
Summary: Our previous research has shown that mice lacking functional GHRH exhibit unique physiological characteristics and an extended lifespan. In this study, we investigated the response to glucagon in these mice and found that they exhibit reduced glucose production and increased energy expenditure. Alterations in pancreatic islet architecture and hepatic glucagon signaling were also observed. These findings suggest that changes in glucagon signaling may contribute to the metabolic phenotype observed in these long-lived mice.
Article
Psychiatry
Despoina Aslanoglou, Suzanne Bertera, Marta Sanchez-Soto, R. Benjamin Free, Jeongkyung Lee, Wei Zong, Xiangning Xue, Shristi Shrestha, Marcela Brissova, Ryan W. Logan, Claes B. Wollheim, Massimo Trucco, Vijay K. Yechoor, David R. Sibley, Rita Bottino, Zachary Freyberg
Summary: DA and NE signaling in the pancreas regulate hormone release, potentially contributing to metabolic disturbances caused by APDs. DA acts as a biased agonist in the pancreas, modulating hormone release. Inhibiting DA receptors can increase hormone release, leading to metabolic disturbances.
TRANSLATIONAL PSYCHIATRY
(2021)
Article
Physiology
Vijaya Subramanian, Jonatan I. Bagger, Jens J. Holst, Filip K. Knop, Tina Vilsboll
Summary: This paper develops a delay differential equation-based model to describe the dynamics of glucose-insulin-glucagon in type 2 diabetes (T2D). The study finds impaired glucose-dependent glucagon suppression and reduced insulin action in T2D patients. Additionally, there is a negative linear relationship between a glucagon suppression parameter and markers for diabetes.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Kyle D. Medak, Alyssa J. Weber, Hesham Shamshoum, Greg L. McKie, Margaret K. Hahn, David C. Wright
Summary: This study aimed to determine if increasing endogenous GLP1 is sufficient to protect against acute olanzapine-induced impairments in glucose and lipid homeostasis. The researchers found that pharmacological approaches to increase endogenous GLP1 can reduce lipid and glucose metabolism disturbances caused by olanzapine. These findings suggest that increasing endogenous GLP1 through pharmacological means may be a useful adjunct approach to mitigate olanzapine-induced perturbations in lipid and glucose metabolism.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Cameron S. Lynch, Victoria C. Kennedy, Amelia R. Tanner, Asghar Ali, Quinton A. Winger, Paul J. Rozance, Russell Anthony
Summary: In the ruminant placenta, GLUT1 and GLUT3 play important roles in glucose uptake and transfer. A deficiency in GLUT3 can result in fetal hypoglycemia and growth restriction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Endocrinology & Metabolism
Jasleen Kaur, Elizabeth R. Seaquist
Summary: Glucagon, secreted by the pancreatic alpha cell, opposes insulin action and has been used for decades to treat insulin-induced hypoglycemia in people with diabetes. Newer formulations of glucagon, such as stable room temperature devices, have made it easier for caregivers to utilize. This review explores the role of glucagon in normal physiology and diabetes, as well as its translation into metabolic condition treatment.
JOURNAL OF ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Olga Kubrak, Takashi Koyama, Nadja Ahrentlov, Line Jensen, Alina Malita, Muhammad T. Naseem, Mette Lassen, Stanislav Nagy, Michael J. Texada, Kenneth Halberg, Kim Rewitz
Summary: This study investigates how the gut regulates metabolism and behavior through gut-derived hormones to maintain energy balance in fruit flies. The researchers found that the gut senses nutrient stress and secretes allatostatin C, which then activates the secretion of glucagon-like adipokinetic hormone to coordinate food intake and energy mobilization. Loss of this regulatory mechanism impairs lipid and sugar mobilization and leads to hypoglycemia.
NATURE COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Xi Chen, Aurora Merovci, Ralph A. DeFronzo, Devjit Tripathy
Summary: A chronic physiologic increase in plasma glucose concentration for 48-72 hours impairs insulin-mediated suppression of glucagon secretion and causes whole body insulin resistance, indicating the importance of glucotoxicity in the development of hyperglucagonemia in type 2 diabetes mellitus.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2023)
Article
Nutrition & Dietetics
Carme Grau-Bove, Iris Gines, Raul Beltran-Debon, Ximena Terra, M. Teresa Blay, Montserrat Pinent, Anna Ardevol
Summary: The endocrine pancreas is influenced by GSPE treatment, with continued effects on glucagon and increased GLP-1 response and receptor expression in the pancreas. When GSPE treatment is given every two weeks, the behavior of the endocrine pancreas changes, with glucagon being a more sensitive parameter to GSPE treatments, dependent on GLP-1 functionality.
Article
Cell Biology
Nicolas Damond, Stefanie Engler, Vito R. T. Zanotelli, Denis Schapiro, Clive H. Wasserfall, Irina Kusmartseva, Harry S. Nick, Fabrizio Thorel, Pedro L. Herrera, Mark A. Atkinson, Bernd Bodenmiller
Article
Multidisciplinary Sciences
Kenichiro Furuyama, Simona Chera, Leon van Gurp, Daniel Oropeza, Luiza Ghila, Nicolas Damond, Heidrun Vethe, Joao A. Paulo, Antoinette M. Joosten, Thierry Berney, Domenico Bosco, Craig Dorrell, Markus Grompe, Helge Raeder, Bart O. Roep, Fabrizio Thorel, Pedro L. Herrera
Review
Cell & Tissue Engineering
Valentina Cigliola, Luiza Ghila, Simona Chera, Pedro L. Herrera
Article
Endocrinology & Metabolism
Shinsuke Tokumoto, Daisuke Yabe, Hisato Tatsuoka, Ryota Usui, Muhammad Fauzi, Ainur Botagarova, Hisanori Goto, Pedro Luis Herrera, Masahito Ogura, Nobuya Inagaki
Article
Biochemical Research Methods
Nils Eling, Nicolas Damond, Tobias Hoch, Bernd Bodenmiller
Article
Endocrinology & Metabolism
Heeyoung Chae, Robert Augustin, Eva Gatineau, Eric Mayoux, Mohammed Bensellam, Nancy Antoine, Firas Khattab, Bao-Khanh Lai, Davide Brusa, Birgit Stierstorfer, Holger Klein, Bilal Singh, Lucie Ruiz, Michael Pieper, Michael Mark, Pedro L. Herrera, Fiona M. Gribble, Frank Reimann, Anne Wojtusciszyn, Christophe Broca, Nano Rita, Lorenzo Piemonti, Patrick Gilon
MOLECULAR METABOLISM
(2020)
Article
Multidisciplinary Sciences
Geoffrey Gaulier, Quentin Dietschi, Swarnendu Bhattacharyya, Cedric Schmidt, Matteo Montagnese, Adrien Chauvet, Sylvain Hermelin, Florence Chiodini, Luigi Bonacina, Pedro L. Herrera, Ursula Rothlisberger, Ivan Rodriguez, Jean-Pierre Wolf
Summary: Experiments have shown that the initial step of vision involves ultra-fast photoisomerization controlled by shaping the phase of femtosecond laser pulses. The electric signals fired from the retina of living mice in response to light stimulation on a femtosecond time scale were found to be sensitive to manipulation of light excitation. This sensitivity is attributed to interactions with light pulses near conical intersections, involving processes like pump-dump and pump-repump.
Article
Biotechnology & Applied Microbiology
Daniel Oropeza, Valentina Cigliola, Agustin Romero, Simona Chera, Santiago A. Rodriguez-Segui, Pedro L. Herrera
Summary: The research demonstrates that alpha-cells undergo stage-specific transcriptional changes at 5 and 15 days post-beta-cell ablation through diphtheria toxin (DT). At day 5, alpha-cells transiently upregulate genes related to interferon signaling and proliferation, while at day 15 they experience a temporary downregulation of genes involved in insulin receptor, mTOR, and MET signaling, indicating potential novel markers and modulated signaling pathways in alpha-cells following acute beta-cell loss.
Article
Multidisciplinary Sciences
Marta Perez-Frances, Leon van Gurp, Maria Valentina Abate, Valentina Cigliola, Kenichiro Furuyama, Eva Bru-Tari, Daniel Oropeza, Taina Carreaux, Yoshio Fujitani, Fabrizio Thorel, Pedro L. Herrera
Summary: Pancreatic polypeptide (Ppy)-expressing gamma-cells are a rare and heterogeneous cell type in the islets, some of which can produce multiple hormones. In response to beta-cell injury, gamma-cells show adaptive plasticity by engaging in insulin production. Loss of Ppy gene or gamma-cells in mice does not affect weight or glycemia under basal conditions.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Marta Perez-Frances, Maria Valentina Abate, Delphine Baronnier, Philipp E. Scherer, Yoshio Fujitani, Fabrizio Thorel, Pedro L. Herrera
Summary: This study investigates the developmental dynamics of pancreatic islet endocrine cell types using murine models. The researchers find that adult islet cells originate from embryonic hormone-expressing cells and there is no evidence of islet cell differentiation from precursor cells after birth. The study also reveals specific patterns of hormone gene activation and switching during islet morphogenesis.
Article
Multidisciplinary Sciences
Johanna Wagner, Markus Masek, Andrea Jacobs, Charlotte Soneson, Sujana Sivapatham, Nicolas Damond, Natalie de Souza, Mark D. Robinson, Bernd Bodenmiller
Summary: In this study, the EMT transition phenotypes in four non-cancerous human mammary epithelial cell lines were comprehensively profiled and compared to breast cancer cell lines. The results revealed a spectrum of EMT transition phenotypes in each cell line. These findings contribute to a better understanding of the complexity of EMT and provide valuable insights for the design of EMT time course experiments.
Article
Multidisciplinary Sciences
Leon van Gurp, Leon Fodoulian, Daniel Oropeza, Kenichiro Furuyama, Eva Bru-Tari, Anh Nguyet Vu, John S. Kaddis, Ivan Rodriguez, Fabrizio Thorel, Pedro L. Herrera
Summary: The authors used single-cell transcriptomics meta-analysis to construct gene sets that accurately describe the identity of various human islet cells. These gene sets have proven effective in analyzing cell identity changes and their underlying genetic mechanisms, providing reliable tools for cell therapy and diabetes treatment.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Preksha Bhagchandani, Charles A. Chang, Weichen Zhao, Luiza Ghila, Pedro L. Herrera, Simona Chera, Seung K. Kim
Summary: This study introduces a new model of experimental diabetes, the B6 RIP-DTR mouse, which can efficiently induce diabetes and be used for islet transplantation. The model has reliable diabetes induction and the rejection response of transplanted islets can be monitored through blood glucose and histology. Different immune cells can be distinguished using flow cytometry in this model.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Judith Magenheim, Miguel Angel Maestro, Nadav Sharon, Pedro L. Herrera, L. Charles Murtaugh, Janel Kopp, Maike Sander, Guoqiang Gu, Douglas A. Melton, Jorge Ferrer, Yuval Dor
Summary: Understanding the origin of pancreatic 0 cells is crucial for diabetes regenerative therapies. Previous beliefs that adult pancreatic duct cells serve as endocrine progenitors have been challenged by lineage-tracing experiments. Recent research suggests that adult pancreatic ducts do contain endocrine progenitors, but alternate interpretations argue that these experiments directly label adult islet somatostatin-producing 0 cells, hindering the assessment of duct cell origin for 0 cells. Overall, evidence indicates that the boundaries between endocrine and exocrine lineages are rarely crossed in the adult pancreas.
