期刊
REGENERATIVE THERAPY
卷 5, 期 -, 页码 17-24出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.reth.2016.06.001
关键词
Rat bone marrow cells; Radial flow bioreactor; Osteogenic differentiation factor; Basic fibroblast growth factor; Proliferation; Differentiation
资金
- Japan Society for the Promotion of Science [24390446]
- Grants-in-Aid for Scientific Research [24390446, 16K11660] Funding Source: KAKEN
Dynamic cultivation using a radial flow bioreactor (RFB) has gained increasing interest as a method of achieving bone regeneration. In order to enhance bone generation in large bone defects, it is necessary to use an RFB to expand the primary cells such as bone marrow cells derived from biotissue. The present study aimed to evaluate the cell expansion and osteogenic differentiation of rat bone marrow cells (rBMC) when added to basic fibroblast growth factor containing medium (bFGFM) or osteogenic differentiation factor containing medium (ODM) under dynamic cultivation using an RFB. Cell proliferation was evaluated with a DNA-based cell count method and histological analysis. An alkaline phosphatase (ALP) activity assay and immunohistochemistry staining of osteogenic markers including BMP-2 and osteopontin were used to assess osteogenic differentiation ability. After culture for one week, rBMC cell numbers increased significantly under dynamic cultivation compared with that under static cultivation in all culture media. For different culture media in dynamic cultivation, bFGFM had the highest increase in cell numbers. ALP activity was facilitated by dynamic cultivation with ODM. Furthermore, both BMP-2 and osteopontin were detected in the dynamic cultivation with ODM. These results suggested that bFGFM promotes cell proliferation and ODM promotes osteogenic differentiation of rBMC under dynamic cultivation using an RFB. (C) 2016, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
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