期刊
VIROLOGICA SINICA
卷 31, 期 6, 页码 490-499出版社
SPRINGER
DOI: 10.1007/s12250-016-3831-4
关键词
HearNPV F protein; monoclonal antibody; 38F10; 44D11; epitope; neutralizing activity; membrane fusion
类别
资金
- National Science Foundation of China [31370191, 31621061]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB11030400]
- Open Research Fund Program of the Key Laboratory of Agricultural and Environmental Microbiology, Chinese Academy of Sciences
The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor (F-0) and then cleaved into a disulfide-linked F-1 and F-2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies (mAbs) against the F-2 subunit of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (HaF) were generated. Two kinds of mAbs were obtained according to their different recognition epitopes: one kind of mAbs, as represented by 38F10, recognizes amino acid (aa) 85 to 123 of F-2 and the other kind, represented by 44D11, recognizes aa 148 to 173 of F-2. Western blotand immunofluo rescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the HaF protein. The results imply that the monoclonal antibody 44D11 recognizes a region within HaF2 that may be involved in the F-mediated membrane fusion process.
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