Article
Ecology
Nana Y. D. Ankrah, Rebecca A. Wilkes, Freya Q. Zhang, Dantong Zhu, Tadeo Kaweesi, Ludmilla Aristilde, Angela E. Douglas
Review
Biochemical Research Methods
Caroll M. Mendonca, Rebecca A. Wilkes, Ludmilla Anstilde
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Article
Agriculture, Multidisciplinary
Mina Solhtalab, Annaleise R. Klein, Ludmilla Aristilde
Summary: Studies have found that phytase-type enzymes play a role in recycling P-i from a heterogeneous assembly of P-o compounds, with different structures of P-o compounds showing varying hydrolysis rates. Molecular docking simulations have shown the interaction mechanisms and energies of each P-o compound within the enzyme's active site, providing insights into the substrate hierarchy.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Engineering, Environmental
Bahareh Hassanpour, Ludmilla Aristilde
Summary: This study utilized exometabolomics approach coupled with C-13 profiling to probe metabolic activities in soil following a C-13-glucose pulse, revealing redox-dependent metabolic dynamics and pathways under oxic or anoxic conditions.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Annaleise R. Klein, Eirini Sarri, Sabrina E. Kelch, Jade J. Basinski, Shreya Vaidya, Ludmilla Aristilde
Summary: Beta-lactam antibiotics, commonly found in surface waters, exhibit different hydrolysis rates and products based on their chemical structures, with cephems like cephalexin and ceftriaxone showing hydrolysis at pH 7.0 and pH 5.0 respectively. The adsorption capacity of smectite clay particles for antibiotics varied with pH and the presence of dissolved organic matter, affecting the fate of the antibiotics in the environment.
ACS EARTH AND SPACE CHEMISTRY
(2021)
Article
Engineering, Environmental
Yeonsoo Park, Christos D. Malliakas, Qing Zhou, April Z. Gu, Ludmilla Aristilde
Summary: This study investigated the interaction of physiologically relevant metal cations with polyphosphate complexes in polyphosphate-accumulating organisms (PAOs), revealing that Magnesium (Mg) and Potassium (K) have a significant impact on the stability of the complexes. Molecular dynamics simulations showed that Mg-polyphosphate complexes were the most stable, while K-polyphosphate complexes were the least stable, suggesting a role in polyphosphate bioavailability. This research provides insights into the mechanistic role of metal complexation in the phosphorus cycling traits of PAOs in engineered and natural systems.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Sabrina E. Kelch, Randall E. Youngman, Eric Ferrage, Jade J. Basinski, Jiaxing Wang, Ludmilla Aristilde
Summary: In this study, Na-23 solid-state nuclear magnetic resonance (NMR) and thermogravimetric analysis-coupled mass spectrometry (TGA-MS) were used to quantitatively analyze hydrated natural Na-montmorillonite at different relative humidity (RH) conditions. The results revealed variations in interlayer structures under different humidity conditions, with different Na+ population ratios and water loss proportions observed.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Soil Science
Michael J. Glaser, Brian K. Richards, Tammo S. Steenhuis, Ludmilla Aristilde
Summary: Phosphonate-based herbicides, including glyphosate, glufosinate, and fosamine, can mobilize and be transported in quartz-enriched agricultural soils due to their interactions with calcium and sodium. The mobility of glyphosate was found to be influenced by both the mineral interfaces and the initial wetness of the soil. These findings provide new insights into the soil mobility of phosphonate-based herbicides and highlight the importance of considering soil properties for their effective and sustainable use.
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL
(2022)
Review
Environmental Sciences
Yeonsoo Park, Mina Solhtalab, Wiriya Thongsomboon, Ludmilla Aristilde
Summary: Soil bacteria employ various strategies to overcome the limitation of inorganic phosphorus in soils by secreting extracellular enzymes to degrade organic phosphorus and utilizing specific transporters and intracellular metabolic pathways for phosphorus recycling. The activities of phosphatases are influenced by soil environmental conditions. Regulatory mechanisms play a crucial role in the production of phosphatases, expression of transporters, and metabolic changes in response to phosphorus availability.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Microbiology
Rebecca A. Wilkes, Jacob Waldbauer, Ludmilla Aristilde
Summary: This study elucidates the metabolic mechanisms facilitating gluconeogenic fast-growth phenotypes in Pseudomonas putida and Comamonas testosteroni, two Proteobacteria with distinct metabolic networks. P. putida relies on a cyclic connection between the Entner-Doudoroff and Embden-Meyerhoff-Parnas pathways, while C. testosteroni bypasses the oxidative pentose phosphate pathway during gluconeogenic growth. Both species deplete protein investment into connected glycolysis pathways to minimize futile carbon cycling and favor gluconeogenic metabolism.
Article
Chemistry, Multidisciplinary
Spencer R. Moller, Yuriy Sakhno, Ludmilla Aristilde, Ruth E. Blake, Deb P. Jaisi
Summary: This study quantified the oxygen isotope exchange kinetics between Pi isotopologues and water using Raman spectroscopy and 31P nuclear magnetic resonance (NMR) during the PPase-catalyzed 18O-16O isotope exchange reaction. The results showed that both Raman spectroscopy and NMR methods are equally applicable for monitoring the oxygen exchange kinetics between Pi isotopologue and water. However, the reliability of these methods is lower below 1% abundance and requires high concentrations of phosphorus (P).
ACS EARTH AND SPACE CHEMISTRY
(2022)
Article
Engineering, Environmental
Bahareh Hassanpour, Neal Blair, Ludmilla Aristilde
Summary: The molecular composition of dissolved organic matter (DOM) in water exhibits variability that cannot be fully captured by stoichiometric ratio-based classification. This study employed metabolomics analysis to uncover patterns in the DOM composition and identified key molecular subgroups that play important roles in carbon transport and cycling dynamics.
Article
Engineering, Environmental
Mina Solhtalab, Spencer R. Moller, April Z. Gu, Deb Jaisi, Ludmilla Aristilde
Summary: This study reveals a hierarchy in enzymatic phosphorus recycling from P-polymers by phosphatases from different biological origins, thereby influencing the relatively longer residence time of RNA versus polyP in environmental matrices.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rebecca A. Wilkes, Jacob Waldbauer, Austin Caroll, Manuel Nieto-Dominguez, Darren J. Parker, Lichun Zhang, Adam M. Guss, Ludmilla Aristilde
Summary: Microbial platforms are crucial for a sustainable energy future and can process aromatic carbons from lignin and plastic feedstocks. Comamonas species are promising bacterial candidates due to their ability to use natural and xenobiotic aromatic compounds, and they often have genetic constraints that prevent competition with sugars. However, the metabolic reactions of these species and their regulatory mechanisms are not well understood.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Engineering, Environmental
Wenting Li, Rebecca A. Wilkes, Ludmilla Aristilde
Summary: Using high-resolution mass spectrometry and a metabolomics approach, this study found that exposure to phosphonate herbicides affects the production of beneficial compounds in plant growth-promoting rhizobacteria, especially under iron-deficient conditions.
ACS ENVIRONMENTAL AU
(2021)