Article
Immunology
Rosanna Leuzzi, Margherita Bodini, Isaac P. Thomsen, Elisabetta Soldaini, Erika Bartolini, Alessandro Muzzi, Bruna Clemente, Bruno Galletti, Andrea Guido Oreste Manetti, Cinzia Giovani, Stefano Censini, Sonia Budroni, Fabiana Spensieri, Erica Borgogni, Silvia Rossi Paccani, Immaculada Margarit, Fabio Bagnoli, Giuseppe Del Giudice, Clarence B. Creech
Summary: The study identified specific cytokine and functional antibody signatures in patients with different primary invasive diseases caused by Staphylococcus aureus. These data provide insights into human responses to invasive staphylococcal infections and are important for guiding the identification of novel preventive and therapeutic interventions against S. aureus. Our findings showed distinct differences in inflammatory responses and antibody levels between patients with invasive S. aureus disease and healthy donors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Xiao-Yan Wang, Jian-Yu Yang, Yi-Ting Wang, Hui-Chao Zhang, Ming-Li Chen, Ting Yang, Jian-Hua Wang
Summary: A surface-enhanced Raman scattering (SERS) nanoprobe based on M13 phages was developed for selective detection and inactivation of Staphylococcus aureus. This method allowed for quantification of S. aureus in aqueous medium and showed promising potential for multifunctional platform for simultaneous detection and inactivation of S. aureus, including bactericidal capabilities.
Article
Chemistry, Analytical
Afang Zhu, Shujat Ali, Zhen Wang, Tianhui Jiao, Qin Ouyang, Quansheng Chen
Summary: A highly structured SERS biosensor was developed for the detection of Staphylococcus aureus. The biosensor utilized a chemically modified PDMS film as the SERS substrate, with self-assembled Au@Ag NPs for enhancing detection sensitivity. The bacterial surface was covered with Au@Ag NPs, resulting in more hot spots for improved detection. The biosensor achieved an ultra-low detection response of 16 cfu/mL and showed satisfactory results in the detection of S. aureus in tap water, milk, and fish samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Wenya Wei, Suleiman A. Haruna, Yumeng Zhao, Huanhuan Li, Quansheng Chen
Summary: This study proposed a SERS biosensor for detecting and quantifying foodborne infections caused by Staphylococcus aureus. The biosensor utilized SiO2 coated Au@Ag nanoparticles and van grafted PDMS to achieve efficient capture of S. aureus and improved repeatability through simplified separation procedures. The biosensor demonstrated good linear correlation and detection sensitivity, and showed potential application for monitoring S. aureus.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xiaoyuan Ma, Xichi Lin, Xumin Xu, Zhouping Wang
Summary: The simultaneous detection of Salmonella typhimurium and Staphylococcus aureus was achieved using aptamer-facilitated gold/silver nanodimer SERS probes, showing high selectivity and accuracy.
Article
Spectroscopy
Xiaoyuan Ma, Shan Xu, Liangyu Li, Zhouping Wang
Summary: In this study, an aptamer-based surface-enhanced Raman spectroscopy (SERS) method was developed for the detection of Staphylococcus aureus without immobilization. The method demonstrated high selectivity and sensitivity, with a low detection limit. It eliminated the need for bacterial immobilization, making it more convenient and efficient for detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Virology
Michele M. Molendijk, My V. T. Phan, Lonneke G. M. Bode, Nikolas Strepis, Divyae K. Prasad, Nathalie Worp, David F. Nieuwenhuijse, Claudia M. E. Schapendonk, Bouke K. H. L. Boekema, Annelies Verbon, Marion P. G. Koopmans, Miranda de Graaf, Willem J. B. van Wamel
Summary: Infections caused by antibiotic resistant Staphylococcus aureus pose a significant challenge to treatment, and phages could potentially serve as an alternative to antibiotics. However, the lack of correlation between conventional in vitro and in vivo phage susceptibility assays hinders the use of phage therapy. This study assessed the susceptibility of twelve S. aureus strains to phages using four assays, and found that microcalorimetry in human serum better mimicked in vivo conditions and could be used to predict the in vivo susceptibility of S. aureus to phages.
Article
Immunology
Elena Boero, Iris Brinkman, Thessely Juliet, Eline van Yperen, Jos A. G. van Strijp, Suzan H. M. Rooijakkers, Kok P. M. van Kessel
Summary: Neutrophils play a crucial role in the immune response to Staphylococcus aureus infections by rapidly migrating to the infection site to engulf and destroy bacteria. A high-throughput flow cytometry assay was described to quantify phagocytosis of S. aureus by human neutrophils, showing that opsonization greatly enhances bacterial uptake. This rapid assay can be used to study neutrophil deficiencies and bacterial evasion, as well as to assess the opsonic capacity of antibodies in natural immune responses or therapies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
I. Ivanovic, R. Boss, A. Romano, E. Guedon, Y. Le-Loir, M. Luini, H. U. Graber
Summary: Staphylococcus aureus is a major pathogen causing mastitis in cattle, and early diagnosis and adequate therapy are crucial. However, there are discrepancies in penicillin resistance testing methods for bovine S. aureus strains, and the best method is still unknown.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Microbiology
Sebastian C. Herren, Markus Huemer, Claudio T. Acevedo, Alejandro Gomez-Mejia, Federica Andreoni, Srikanth Mairpady Shambat, Barbara Hasse, Reinhard Zbinden, Silvio D. Brugger, Annelies S. Zinkernagel
Summary: Antibiotic-tolerant Staphylococcus aureus poses a great challenge to clinicians and microbiological laboratories, with fast and reliable methods such as the replica plating tolerance isolation system (REPTIS) being urgently required for detection. This system was found to be useful in detecting antibiotic tolerance in clinical microbiological routine diagnostics, with high regrowth observed in antibiotics targeting protein synthesis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Review
Pharmacology & Pharmacy
Kannappan Arunachalam, Poonguzhali Pandurangan, Chunlei Shi, Ricardo Lagoa
Summary: Staphylococcus aureus is a versatile pathogen that causes various infections. It is important to understand its pathogenesis and develop new treatment strategies, especially against drug-resistant strains. Nanomaterials offer a potential solution to control S. aureus infections, including intracellular parasitism and multidrug resistance. This review focuses on the impact of S. aureus on human health, the role of virulence factors and biofilms, the applications of nanoparticles in controlling infections, and the prospects and limitations of nanomaterial-based therapies.
Article
Biophysics
Neeraja Ravi, Sarah E. Chang, Luis M. Franco, Sandesh C. S. Nagamani, Purvesh Khatri, Paul J. Utz, Shan X. Wang
Summary: Detecting and quantifying the host transcriptional response to influenza virus infection can serve as a real-time diagnostic tool for clinical management. We have developed a novel assay based on the influenza metasignature (IMS) using GMR sensors, which can classify influenza infection based on transcript levels. We also validated the accuracy of a single biomarker in stratifying patients with influenza.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Agriculture, Dairy & Animal Science
Jiai Yan, Ruijin Yang, Suhuai Yu, Wei Zhao
Summary: Staphylococcus aureus is a prevalent foodborne pathogen that poses a threat to human health, especially with the emergence of multidrug-resistant strains like MRSA. The study found that the endolysin LysGH15 and its catalytic domain CHAP(LysGH15) exhibited strong antimicrobial efficacy against S. aureus, with differing optimal activity conditions. The sensitivity to NaCl concentration also varied between LysGH15 and CHAP(LysGH15), affecting their lytic activity levels.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Orthopedics
Wei Fu, Wenbin He, Youliang Ren, Zhengdao Li, Jinyue Liu, Yi Liu, Zhao Xie, Jianzhong Xu, Qing Bi, Mingxiang Kong, Charles C. Lee, John L. Daiss, Gowrishankar Muthukrishnan, John R. Owen, Stephen L. Kates, Jiachen Peng, Chao Xie
Summary: The study highlights that dynamic changes in specific antigens of Staphylococcus aureus osteomyelitis patients can serve as predictive markers for clinical outcomes.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Yinjuan Xu, Lixia Shi, Xiaohui Jing, Hongyan Miao, Yuan Zhao
Summary: A new SERS immunoassay method was developed for the detection of Staphylococcus aureus enterotoxins using plasmonic Au-Ag Janus nanoparticles and CsPb2Br5@MSNs composites, which showed amplified SERS activity and a positive relationship with the toxin concentration. This simple, sensitive, and reproducible method has the potential to be applied in other food safety monitoring.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Critical Care Medicine
C. Gabara, B. Solarat, P. Castro, S. Fernandez, J. R. Badia, D. Toapanta, S. Schulman, J. C. Reverter, A. Soriano, J. Moises, J. Aibar
Summary: This study evaluated the rates of thrombosis, bleeding, and mortality in critically ill COVID-19 patients receiving different anticoagulant doses. The study found that bleeding events were more frequent in the intermediate and therapeutic dose groups, but there were no significant differences in thrombosis and mortality rates.
MEDICINA INTENSIVA
(2023)
Article
Immunology
Rodrigo Alonso-Navarro, Genoveva Cuesta, Marta Santos, Celia Cardozo, Veronica Rico, Nicole Garcia-Pouton, Montse Tuset, Marta Bodro, Laura Morata, Pedro Puerta-Alcalde, Sabina Herrera, Dafne Soria, Marta Aldea, Josep Mensa, Jose Antonio Martinez, Ana Del Rio, Jordi Vila, Felipe Garcia, Carolina Garcia-Vidal, M. Angeles Marcos, Alex Soriano
Summary: A prospective study found that negative subgenomic RNA (sgRNA) at admission or after 5 days of remdesivir therapy was associated with better outcomes in hospitalized patients with moderate/severe COVID-19. Monitoring sgRNA levels could be useful in assessing the response to antiviral treatment.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Orthopedics
Caribay Vargas-Reveron, Lluis Font-Vizcarra, Laura Morata, Jenaro A. Fernandez-Valencia, Alex Soriano, Ernesto Munoz-Mahamud
Summary: The association between unexpectedly positive culture results during elective hip arthroplasty and the risk of subsequent premature revision within 5 years is not known.
CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
(2023)
Article
Immunology
Blanca Molins, Marc Figueras-Roca, Oliver Valero, Victor Llorenc, Sara Romero-Vazquez, Oriol Sibila, Alfredo Adan, Carolina Garcia-Vidal, Alex Soriano
Summary: In COVID-19 patients, monomeric CRP levels are associated with disease severity, and levels higher than 4000 ng/mL independently predict disease severity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Infectious Diseases
Nada S. Shabana, Gesine Seeber, Alex Soriano, Paul C. Jutte, Silvia Westermann, Glenn Mithoe, Loredana Pirii, Theke Siebers, Bas ten Have, Wierd Zijlstra, Djordje Lazovic, Marjan Wouthuyzen-Bakker
Summary: This study aimed to assess the antibiotic resistance patterns of Staphylococcus epidermidis in early periprosthetic joint infections (PJI) and correlate with clinical outcomes. Results showed that methicillin and ciprofloxacin resistance were associated with higher clinical failure rates. Reduced susceptibility to vancomycin was also related to a higher failure rate. Resistance to fluoroquinolones was identified as an independent risk factor for clinical failure. Alternative prophylactic antibiotics and optimized treatment strategies are needed to improve outcomes.
Article
Chemistry, Multidisciplinary
Tolga Zorlu, I. Brian Becerril-Castro, Begona Puertolas, Vincenzo Giannini, Miguel A. Correa-Duarte, Ramon A. Alvarez-Puebla
Summary: Hybrid composites of metal organic frameworks (MOFs) and nanoparticles are considered optimal materials for various applications. A newly developed method involves preparing a yolk-shell structure consisting of a plasmonic gold nanostar coated with MOF. This configuration exhibits improved colloidal stability, the ability to separate molecules based on size or charge, possible synergy for photocatalysis when combined with gold, and strong optical activity for use as SERS sensors.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Rosalia Sanchez-Fernandez, Agustin Sanchez-Temprano, David Esteban-Gomez, Elena Pazos
Summary: Tyrosine nitration is a post-translational modification that occurs under nitrosative stress conditions through a non-enzymatic peroxynitrite-mediated reaction. It is considered both an irreversible modification involved in nitrosative stress-associated diseases and a regulatory mechanism of signal transduction. In this study, a Tb-III-chelating metallopeptide was explored as a tool to monitor tyrosine nitration. The luminescence intensity of this probe significantly decreases after nitration, and this change is directly related to the degree of nitration. Importantly, the changes in luminescence intensity are not affected by complex biological media, making it a promising tool for understanding this protein modification.
Review
Chemistry, Physical
Tolga Zorlu, Miguel A. A. Correa-Duarte, Ramon A. A. Alvarez-Puebla
Summary: In recent years, metal-organic frameworks, especially zeolitic imidazolate frameworks, have gained popularity due to their large surface area, homogeneous pore structure, and easy integration with plasmonic nanoparticles for the production of optical sensors. This article summarizes the latest advances in using these hybrid composites in surface-enhanced Raman scattering and discusses their future perspectives.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Organic
Pablo Corton, Natalia Fernandez-Labandeira, Mauro Diaz-Abellas, Carlos Peinador, Elena Pazos, Arturo Blanco-Gomez, Marcos D. Garcia
Summary: A new polycationic pseudo[1]rotaxane was successfully synthesized through hydrazone bonds, self-assembled in excellent yield in aqueous media. The thermodynamically controlled process involves the [1 + 1] reaction of a bisaldehyde and a trishydrazine, formation of the macrocyclic part, conversion into pseudo[1]rotaxane, and the one-pot [1 + 1 + 1] condensation process. The obtained molecule exhibited the ability to form self-inclusion complexes and supramolecular aggregates in different concentration ranges.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Seyed Farzad Baniahmad, Romane Oliverio, Ines Obregon-Gomez, Alma Robert, Anne E. G. Lenferink, Elena Pazos, Nick Virgilio, Xavier Banquy, Gregory De Crescenzo, Yves Durocher
Summary: Long-term delivery is a successful strategy to reduce adverse effects of mAb-based treatments. Macroporous hydrogels and affinity-based strategies are effective in sustained and localized delivery of mAbs. The Ecoil and Kcoil peptides have high-affinity binding and can be used for controlled release of Ecoil-tagged trastuzumab from macroporous hydrogels.
Article
Chemistry, Organic
Carlota Giron-Elola, Ibon Sasiain, Rosaliia Sanchez-Fernandez, Elena Pazos, Arkaitz Correa
Summary: A cross-dehydrogenative coupling reaction of Tyr-containing peptides and estrogens with heteroarenes was developed, allowing for the formation of C-N bonds. This scalable and air-tolerant oxidative coupling reaction has operational simplicity and enables the attachment of phenothiazines and phenoxazines in phenol-like compounds. Incorporation of the Tyr-phenothiazine moiety into a Tb(III) metallopeptide serves as a sensitizer for the Tb(III) ion, providing a new tool for the design of luminescent probes.
Article
Infectious Diseases
Angel Marcos Fendian, Marta Albanell-Fernandez, Montse Tuset, Cristina Pitart, Pedro Castro, Dolors Soy, Marta Bodro, Alex Soriano, Ana del Rio, Jose Antonio Martinez
Summary: We aim to report our early experience in using cefiderocol as a rescue therapy for patients infected with difficult-to-treat pathogens. Our case study suggests that cefiderocol is an effective and safe treatment option for patients with extensively drug-resistant bacteria, although the risk of resistance emergence exists.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Orthopedics
Marta Sabater-Martos, Miguel A. Verdejo, Laura Morata, Ernesto Munoz-Mahamud, Ernesto Guerra-Farfan, Juan C. Martinez-Pastor, Alex Soriano
Summary: In this article, the principles and limitations of local antibiotic delivery are reviewed, with a focus on the potential association between antibiotic-loaded bone cement (ALBC) and antimicrobial resistance. The efficacy of ALBC for prosthetic joint infection (PJI) prophylaxis and treatment is discussed, and concerns regarding the emergence of resistance are addressed. The current evidence does not support an association between the use of ALBC and increased antimicrobial resistance.
Article
Nanoscience & Nanotechnology
Yoel Negrin-Montecelo, Adbelrhaman Hamdeldein Ahmed Geneidy, Alexander O. Govorov, Ramon A. Alvarez-Puebla, Lucas V. Besteiro, Miguel A. Correa-Duarte
Summary: Photocatalysis is a promising alternative energy technology that converts solar energy into chemical products. This study explores the use of plasmonic materials to sensitize traditional semiconductor photocatalysts. Using CdS quantum dots as a bridging system, energy from Au nanostructures is transferred to TiO2 nanoparticles, resulting in significantly accelerated reactions. The study highlights the importance of the morphology of the Au nanoparticles in their photosensitizing capabilities.
Article
Infectious Diseases
Christen Ravn, Jeroen Neyt, Natividad Benito, Miguel Araujo Abreu, Yvonne Achermann, Svetlana Bozhkova, Liselotte Coorevits, Matteo Carlo Ferrari, Karianne Wiger Gammelsrud, Ulf-Joachim Gerlach, Efthymia Giannitsioti, Martin Gottliebsen, Nis Pedersen Jorgensen, Tomislav Madjarevic, Leonard Marais, Aditya Menon, Dirk Jan Moojen, Markus Paakkonen, Marko Pokorn, Daniel Perez-Prieto, Nora Renz, Jesus Saavedra-Lozano, Marta Sabater-Martos, Parham Sendi, Staffan Tevell, Charles Vogely, Alex Soriano
Summary: This guideline provides evidence and opinion-based recommendations for the diagnosis and management of patients with septic arthritis of a native joint. It is intended for use by orthopedic surgeons and physicians who care for these patients.
JOURNAL OF BONE AND JOINT INFECTION
(2023)
