Article
Biochemical Research Methods
Fouad Choueiry, Rui Xu, Jiangjiang Zhu
Summary: Staphylococcus aureus, an opportunistic pathogen, relies on host-derived environmental signals and metabolic adaptation to cause infections. In this study, we investigated the effects of environmental factors on SA metabolism and its correlation with the expression of virulence genes. Our findings provide insights into the adaptive growth and virulence of SA, and may facilitate the development of metabolic inhibitors/modulators to mitigate SA infections.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Microbiology
Saugat Poudel, Ying Hefner, Richard Szubin, Anand Sastry, Ye Gao, Victor Nizet, Bernhard O. Palsson
Summary: The study investigates the regulation of carbon and amino acid metabolism in Staphylococcus aureus through a modular transcriptional regulatory network and metabolic model. The results demonstrate the coordinated regulation of central carbon metabolism and amino acid biosynthesis by CcpA and CodY, as well as the link between metabolic cues and translation through the expression of ribosome hibernation-promoting factor.
Article
Microbiology
Gyu-Lee Kim, Thomas A. Hooven, Javiera Norambuena, Barry Li, Jeffrey M. Boyd, Jason H. Yang, Dane Parker
Summary: Studying the genetic elements of Staphylococcus aureus in pneumonia revealed that metabolic genes are crucial for lung infection, with growth and resistance to host defenses being key metabolic dimensions. This highlights the critical role of bacterial metabolism in surviving against host defenses during infection.
Article
Microbiology
Ye Gao, Saugat Poudel, Yara Seif, Zeyang Shen, Bernhard O. O. Palsson
Summary: The transcription factor CodY plays a crucial role in coordinating metabolism and virulence expression in Staphylococcus aureus USA300. We conducted a comparative analysis of CodY regulation between two dominant USA300 strains, revealing its target genes and providing insights into strain-specific characteristics. This study highlights the importance of characterizing common pathogenic strains and developing targeted treatments for major circulating strains.
Article
Infectious Diseases
Qi Peng, Lu Guo, Yu Dong, Tingrui Bao, Huiyuan Wang, Tao Xu, Ying Zhang, Jian Han
Summary: This study demonstrates the role of PurN in the formation of late exponential phase Staphylococcus aureus persisters and its regulation of virulence through GltB and the SaeRS two-component system.
Article
Microbiology
William R. Schwan
Summary: Staphylococcus aureus requires the importation of proline to survive. Single mutations in proline transporter genes affect the ability of the bacterium to survive, while triple gene mutations significantly hinder the proline transport and growth of S. aureus.
Article
Microbiology
Laura J. Dunphy, Kassandra L. Grimes, Nishikant Wase, Glynis L. Kolling, Jason A. Papin
Summary: In a synthetic cystic fibrosis media, distinct species-specific metabolic signatures were revealed for Pseudomonas aeruginosa and Staphylococcus aureus, indicating potential nutrient competition's impact on community dynamics. Both bacteria consumed similar nutrients, suggesting metabolic competition's role in coinfection dynamics. Furthermore, unique metabolites produced by each species may serve as species-specific biomarkers for cystic fibrosis lung infections.
Article
Microbiology
Q. C. Truong-Bolduc, Y. Wang, D. C. Hooper
Summary: This study demonstrates that S. aureus Tet38 is a tetracycline efflux pump regulated by the KdpD/E regulator. This is the first direct evidence of Tet38-mediated tetracycline efflux, which was previously inferred from its ability to confer tetracycline resistance. Under salt stress, S. aureus adjusts its survival by reducing the expression of tet38 through KdpD/E. The study also shows the differences in function between Tet38 and other tetracycline efflux transporters regarding their transport of tetracycline and Na+/K+. Importantly, sodium selectively reduced tetracycline efflux by Tet38, and potassium selectively reduced tetracycline efflux by TetK. These findings highlight the importance of Tet38 in bacterial adaptation and survival in diverse environments.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Microbiology
Mousa Alreshidi, Hugh Dunstan, Tim Roberts, Fevzi Bardakci, Riadh Badraoui, Mohd Adnan, Mohd Saeed, Fayez Alreshidi, Yazeed Albulaihed, Mejdi Snoussi
Summary: This study investigated the differences in cytoplasmic amino acid profiles of Staphylococcus aureus under various stress-induced conditions. The results showed that the bacterium adapts its metabolic homeostasis and amino acid content to different wound site conditions. This adaptability may contribute to its virulence as an opportunistic pathogenic bacterium invading wounds.
Article
Cell & Tissue Engineering
F. Alagboso, G. K. Mannala, N. Walter, D. Docheva, C. Brochhausen, V Alt, M. Rupp
Summary: Treatment with rifampicin can eradicate intracellular Staphylococcus aureus infection in osteoblasts and restore their metabolic activity and extracellular organic matrix formation and mineralization ability.
BONE & JOINT RESEARCH
(2022)
Review
Microbiology
Laura Barrientos, Noemie Mercier, David Lalaouna, Isabelle Caldelari
Summary: The success of opportunistic human Staphylococcus aureus relies on the production of virulence factors through transcriptional factors, two-component systems, and small regulatory RNAs. High-throughput sequencing technologies have revealed hundreds of potential regulatory RNAs, with sRNAs playing a key role in bacterial physiology and virulence adaptability. These findings highlight the importance of sRNAs in understanding infection processes and raise questions about the interaction between key regulators and the pathways they control.
FRONTIERS IN MICROBIOLOGY
(2021)
Letter
Microbiology
Lisiane da Luz Rocha Balzan, Adriana Medianeira Rossato, Cezar Vinicius Wurdig Riche, Vlademir Vicente Cantarelli, Pedro Alves D'Azevedo, Aline Valerio de Lima, Beatriz Rodrigues, Ivan Leonardo Avelino Franca e Silva, Cicero Armidio Gomes Dias, Jorge Luiz Mello Sampaio
Summary: In 2015, two new species related to Staphylococcus aureus were proposed. We report the isolation of five strains of the newly identified species, Staphylococcus argenteus, from human cases of bacteremia and skin and soft tissue infections. This is the first documentation of S. argenteus causing community-acquired and hospital-acquired infections in South America.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Mafalda Bispo, Sabrina Suhani, Jan Maarten van Dijl
Summary: This study revealed that human serum albumin could reduce the efficacy of targeted antimicrobial photodynamic therapy against MRSA, while the addition of non-toxic potassium iodide could enhance the eradication of MRSA.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Microbiology
Qiyuan Jin, Xiaolu Xie, Yaxuan Zhai, Haifang Zhang
Summary: This review focuses on the mechanisms of folate metabolism and its relation to S. aureus infection. It has been discovered that S. aureus intracellular infection relies on folate metabolism. Lack of folate inhibits nucleotide synthesis in S. aureus, and certain antibiotics disrupt folate metabolism, leading to the production of thymidine-dependent small-colony variants. Additionally, S. aureus becomes resistant to lysostaphin in the presence of serine hydroxymethyltransferase.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Immunology
Mincheng Lu, Ruiyi He, Chao Li, Zixian Liu, Yuhui Chen, Bingsheng Yang, Xianrong Zhang, Bin Yu
Summary: In this study, we investigated the role of ApoE in regulating cholesterol metabolism during S. aureus osteomyelitis. We found that ApoE plays a key role in S. aureus osteomyelitis by enhancing macrophage resistance and reducing bone destruction.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Casey M. Gries, Trevor Biddle, Jeffrey L. Bose, Tammy Kielian, David D. Lo
INFECTION AND IMMUNITY
(2020)
Article
Biochemistry & Molecular Biology
Merve S. Zeden, Igor Kviatkovski, Christopher F. Schuster, Vinai C. Thomas, Paul D. Fey, Angelika Grundling
MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Cortney E. Heim, Megan E. Bosch, Kelsey J. Yamada, Amy L. Aldrich, Sujata S. Chaudhari, David Klinkebiel, Casey M. Gries, Abdulelah A. Alqarzaee, Yixuan Li, Vinai C. Thomas, Edward Seto, Adam R. Karpf, Tammy Kielian
NATURE MICROBIOLOGY
(2020)
Article
Microbiology
Megan E. Bosch, Blake P. Bertrand, Cortney E. Heim, Abdulelah A. Alqarzaee, Sujata S. Chaudhari, Amy L. Aldrich, Paul D. Fey, Vinai C. Thomas, Tammy Kielian
Article
Microbiology
Harim Won, Sean M. Watson, Jong-Sam Ahn, Jennifer L. Endres, Kenneth W. Bayles, Marat R. Sadykov
Summary: The Pta-AckA pathway plays a crucial role in the fitness of the spore-forming bacterium Bacillus anthracis, with disruption leading to growth reduction and metabolic alterations. Inactivation of Pta-AckA pathway increases glucose consumption, affects intracellular ATP and NADH levels, and causes metabolic blockage at key nodes. The study highlights the importance of Pta-AckA pathway in maintaining metabolic homeostasis and could potentially serve as a therapeutic target for drug discovery.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Immunology
Elyza A. Do, Casey M. Gries
Summary: Potassium is essential for the survival and function of both host and pathogenic bacteria during infection, playing a critical role in nutrient uptake, virulence gene expression, antimicrobial resistance, and biofilm formation.
INFECTION AND IMMUNITY
(2021)
Article
Gastroenterology & Hepatology
Koelina Ganguly, Rakesh Bhatia, Sanchita Rauth, Andrew Kisling, Pranita Atri, Christopher Thompson, Raghupathy Vengoji, Shiv Ram Krishn, Dhananjay Shinde, Vinai Thomas, Sukhwinder Kaur, Kavita Mallya, Jesse L. Cox, Sushil Kumar, Surinder K. Batra
Summary: The study reveals that MUC5AC promotes gemcitabine resistance in pancreatic cancer cells by affecting glutamine metabolism and nucleotide biosynthesis. Depletion of MUC5AC and deprivation of glutamine can enhance sensitivity of pancreatic cancer cells to gemcitabine. Dual-targeting therapy against MUC5AC, beta-catenin, and glutamine metabolism can overcome gemcitabine resistance in pancreatic cancer.
Article
Microbiology
Jennifer L. Endres, Sujata S. Chaudhari, Xinyan Zhang, Janani Prahlad, Shu-Qi Wang, Lily A. Foley, Sorin Luca, Jeffrey L. Bose, Vinai C. Thomas, Kenneth W. Bayles
Summary: The Staphylococcus aureuscidABC and lrgAB operons encode proteins with holin-like properties and play an important role in the transport of small metabolic by-products of carbohydrate metabolism.
Article
Microbiology
Itidal Reslane, Cortney R. Halsey, Amanda Stastny, Barbara J. Cabrera, Jongsam Ahn, Dhananjay Shinde, Madeline R. Galac, Margaret F. Sladek, Fareha Razvi, McKenzie K. Lehman, Kenneth W. Bayles, Vinai C. Thomas, Luke D. Handke, Paul D. Fey
Summary: This study reveals that the transcriptional repression of ornithine carbamoyltransferase encoded by arcB1 within the native arginine deiminase operon and limited intracellular pools of ornithine are the main factors repressing arginine biosynthesis in Staphylococcus aureus in media lacking glucose. Surprisingly, approximately 50% of S. aureus clinical isolates can grow in media lacking arginine, suggesting that mutations are selected in S. aureus that allow growth in specific niches of the human host.
Article
Microbiology
Logan L. Bulock, Jongsam Ahn, Dhananjay Shinde, Sanjit Pandey, Cleofes Sarmiento, Vinai C. Thomas, Chittibabu Guda, Kenneth W. Bayles, Marat R. Sadykov
Summary: This study investigates the interplay and regulation of central metabolism and biofilm development in Staphylococcus aureus by two global transcriptional regulators, CodY and CcpA. The results show that the lack of CcpA and/or CodY has different impacts on intracellular metabolic status leading to the formation of morphologically altered biofilms.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Immunology
Blake P. Bertrand, Cortney E. Heim, Sean C. West, Sujata S. Chaudhari, Hesham Ali, Vinai C. Thomas, Tammy Kielian
Summary: Biofilms formed by Staphylococcus aureus on medical devices are a significant healthcare burden, and a type of white blood cell, G-MDSCs, promote biofilm persistence by inhibiting the activity of other immune cells. This study found that S. aureus promotes biofilm formation and survival through formate metabolism.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Emilio Bueno, Victor Pinedo, Dhananjay D. Shinde, Andre Mateus, Athanasios Typas, Mikhail M. Savitski, Vinai C. Thomas, Felipe Cava
Summary: Even though resistance to arsenate has been extensively investigated in environmental bacteria, how enteric pathogens tolerate this toxic compound remains unknown. Here, we found that the cholera pathogen V. cholerae exhibits increased resistance to arsenate compared to closely related enteric pathogens.
Article
Mathematical & Computational Biology
Mohammad Mazharul Islam, Vinai C. Thomas, Matthew Van Beek, Jong-Sam Ahn, Abdulelah A. Alqarzaee, Chunyi Zhou, Paul D. Fey, Kenneth W. Bayles, Rajib Saha
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2020)
Article
Microbiology
Jennifer L. Endres, Sujata S. Chaudhari, Xinyan Zhang, Janani Prahlad, Shu-Qi Wang, Lily A. Foley, Sorin Luca, Jeffrey L. Bose, Vinai C. Thomas, Kenneth W. Bayles
Summary: The proteins encoded by the Staphylococcus aureus cidA and lrgA genes have been shown to possess bacteriophage holin-like functions and are also involved in the transport of small metabolic by-products of carbohydrate metabolism. These findings suggest an intriguing link between these two seemingly disparate processes.